scholarly journals Assessment of Wound Healing Activity of the Aqueous Extracts ofColutea cilicicaBoiss. & Bal. Fruits and Leaves

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ipek Peşin Süntar ◽  
Ufuk Koca ◽  
Esra Küpeli Akkol ◽  
Demet Yılmazer ◽  
Murat Alper
Keyword(s):  
Wound Healing ◽  
Histopathological Examination ◽  
Healing Process ◽  
Wound Healing Process ◽  
Aqueous Extracts ◽  
Reference Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Incision Wound ◽  
Wound Healing Activity

The fruiting branches ofColutea cilicicaBoiss. & Bal., along with leaves and fruits, have been used to heal inflammatory wounds at traditional medicine in various parts of Turkey. In order to evaluate the wound healing activity of the plant, aqueous extracts were prepared from the flowering parts and fruits ofColutea cilicicaBoiss. & Bal. by using 40°C distilled water. Incision wound healing models by using tensiometer on rats and excision wound healing models on mice were employed to assess the activity. Significant wound healing activity was observed when ointment formulation of the aqueous extract at 1% concentration was applied on the mentioned models. The fruit extract treated the groups of animals, showing a 78.1% contraction in wounds, which was close to the contraction value of the reference drug Madecassol (100%). On the other hand, the same extract used on the incision wound model demonstrated a significant increase (42%) in wound tensile strength, compared to the flowering aerial parts. Histopathological examination also supported the results shown in both the incision and excision wound models. The wound healing effect was evaluated and compared with the reference ointment Madecassol. Moreover, zinc and vitamin C levels in the fruit (9480 ± 0 mcg g−1; 389 mg g−1) and flowering aerial part (6609 ± 1 mcg g−1; 404 mg g−1) extracts, which might have contributed to the wound healing process, were determined. The experimental data confirmed the traditional usage ofC. cilicicaBoiss.& Bal.

scholarly journals Evaluation of the Wound Healing Potential ofAchillea biebersteiniiAfan. (Asteraceae) byIn VivoExcision and Incision Models

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Esra Küpeli Akkol ◽  
Ufuk Koca ◽  
Ipek Pesin ◽  
Demet Yilmazer
Keyword(s):  
Wound Healing ◽  
Folk Medicine ◽  
Reference Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Achillea Biebersteinii ◽  
Healing Of Wounds ◽  
Wound Healing Effect ◽  
Incision Wound ◽  
Wound Healing Activity

Achilleaspecies are widely used for diarrhea, abdominal pain, stomachache and healing of wounds in folk medicine. To evaluate the wound healing activity of the plant, extracts were prepared with different solvents; hexane, chloroform, ethyl acetate and methanol, respectively from the roots ofAchillea biebersteinii. Linear incision by using tensiometer and circular excision wound models were employed on mice and rats. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol. Then-hexane extract treated groups of animals showed 84.2% contraction, which was close to contraction value of the reference drug Madecassol (100%). On the other hand the same extract on incision wound model demonstrated a significant increase (40.1%) in wound tensile strength as compared to other groups. The results of histoptological examination supported the outcome of linear incision and circular excision wound models as well. The experimental data demonstrated thatA. biebersteiniidisplayed remarkable wound healing activity.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

WOUND HEALING ACTIVITY OF THE LEAVES OF WRIGHTIA TINCTORIA (ROXB) R. BR

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (03) ◽  
pp. 40-46
Author(s):  
M. C. Divakar ◽  
◽  
S Lakshmi Devi
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Excision Wound ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Wrightia Tinctoria ◽  
Incision Wound ◽  
Wound Healing Activity

The study was aimed to evaluate wound healing activity of 70% ethanolic and methanolic extracts of leaves of Wrightia tinctoria (Roxb) R. Br (Apocynaceae) using incision and excision wound models on Wistar rats. Wound contraction and period of epithelization were assessed in excision wound model whereas wound tensile strength was determined in case of incision wound model. 4% Wrightia tinctoria methanolic leaf extract phytosome exhibited significant wound healing potential when compared with standard 0.2% nitrofurazone ointment.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF POLYHERBAL FORMULATION

2017 ◽  
Vol 9 (6) ◽  
pp. 12
Author(s):  
Madhuri A. Theng ◽  
G. R. Sitaphale ◽  
K. R. Biyani
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Soxhlet Extraction ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Butea Monosperma ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Acacia Arabica ◽  
Wound Healing Activity

Objective: The present study describes the anti-microbial acivity of Acacia arabica and Butea monosperma bark extract.Methods: For this purpose aqueous extract of bark were prepared by “Soxhlet extraction method”. The experimentally induced burn wound model in rats by “Excision method”.Results: As a result of this study it was found that the extract of bark generally revealed antimicrobial and wound healing activity.Conclusion: The result of the study suggest that the Acacia arabica and Butea monosperma bark of polyherbal gel effective in accelerating wound healing process.

scholarly journals Evaluation of in vivo Wound Healing Activity of Ursolic Acid Rich Chloroform Extract of Hedyotis herbacea Linn Ointment

2021 ◽  
pp. 246-255
Author(s):  
K. Anand Babu ◽  
S. Sivakrishnan ◽  
S. Jasemine
Keyword(s):  
Wound Healing ◽  
Ursolic Acid ◽  
Chloroform Extract ◽  
Control Group ◽  
Untreated Control Group ◽  
Excision Wound ◽  
Wound Model ◽  
Incision Wound ◽  
Wound Healing Activity

Aim: This study evaluates the effect of in vivo wound healing potential of Ursolic acid rich chloroform extract of Hedyotis herbacea Linn ointment using incision and excision wound model. Study Design: Wound healing potentials of Hedyotis herbacea were analysed by Incision and excision wound model. Place of Study: Nandha College of Pharmacy, Erode, Tamilnadu. Methodology: Hedyotis herbacea was subjected to extraction (cold maceration), with solvents of increasing polarity. All the extracts were estimated for the presence of phytoconstituents by HPTLC. As the study has been focused on the phytoconstituent based biological activity, the Ursolic acid rich chloroform extract was chosen for the study of wound healing activity. The Chloroform extract of Hedyotis herbacea was incorporated into ointment base, to prepare the ointment. The ointments prepared with Chloroform extract (2.5% and 5%) of Hedyotis herbacea (CEHH) were subjected for evaluation of excision and incision wound model. Wistar albino rats were divided into four groups each consisting of six animals; group I (left untreated) considered as untreated control, group II treated with 5% w/w povidone-iodine ointment served as standard, group III treated with CEHH 2.5% w/w ointment, and group IV treated with CEHH 5% w/w ointment were considered as test groups. All the treatments were given once daily. Wound healing effect was assessed by the rate of wound contraction, level of total protein, Hydroxy proline, Hexosamine and Hexuronic acid and histopathology studies in an excision wound model. Tensile strength was also measured in both excision and incision wound. Results: Wound healing activity of CEHH 5% w/w ointment treated group was greater than CEHH 2.5% w/w and untreated groups in both excision and incision wound model. The high rate of wound contraction (*P< 0.001), high tensile strength (*P< 0.001), and elevated total protein, Hydroxyproline, Hexosamine, and Hexuronic acid content were observed in animals treated with CEHH ointments when compared to the untreated control group of animals. Histopathological studies of the CEHH ointments treated groups also revealed the effectiveness in wound healing. Conclusion: These results justified the claimed traditional use of the Hedyotis herbacea as wound healing plant.

scholarly journals Effects of Alchemilla mollis and Alchemilla persica on the wound healing process

2016 ◽  
Vol 11 (3) ◽  
pp. 577
Author(s):  
Burçin Ergene Öz ◽  
Mert Ilhan ◽  
Serkan Ozbilgin ◽  
Esra Küpeli Akkol ◽  
Özlem Bahadır Acıkara ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Video Clip ◽  
Wound Healing Process ◽  
Histopathological Analysis ◽  
Excision Wound ◽  
Wound Model ◽  
Aerial Parts ◽  
Anti Inflammatory ◽  
Treatment Of Wounds

<p class="Abstract"><em>Alchemilla mollis</em>, is used in traditional medicine for the treatment of wounds and excessive menstruation. Aqueous methanol extracts of<em> A. mollis</em> and <em>A. persica</em> were evaluated for wound healing acivity by using linear incision and circular excision wound models along with hydroxyproline estimation and histopathological analysis. Anti-inflammatory effect was determined according to Whittle method. The extracts prepared from the aerial parts of <em>A. mollis</em> and <em>A. persica</em> exerted significant wound healing activity with the tensile strength values of 39.3% and 33.3%, respectively, and with the contraction values of 51.4% and 43.5%, respectively. Hydroxyproline estimation and histopathological analysis also confirmed the results. The extracts of <em>A. mollis</em> and <em>A. persica</em> showed significant anti-inflammatory activity with the values of 30.6% and 26.6% respectively. These results showed that <em>A. mollis</em> and <em>A. persica</em> possess significant wound healing and anti-inflammatory activities.</p><p class="Abstract"><strong>Video Clip</strong></p><p class="Subheading"><a href="https://youtube.com/v/k2Hz7f1e4SI">Circular excision wound model</a>: 2 min</p><p> </p>

scholarly journals WOUND HEALING ACTIVITY OF JATROPHA TANJORENSIS LEAVES

2018 ◽  
Author(s):  
Madepalli Byrappa gowdu Viswanathan ◽  
Jesu Doss Jeya Ananthi ◽  
NataRajan Livingston Raja ◽  
Narayanan Venkateshan
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Methanol Extract ◽  
Chloroform Extract ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
And Function ◽  
Incision Wound ◽  
Wound Healing Activity

The term “wound” is defined as a disruption of normal anatomical structure. Therefore, “healing” is the complex and dynamic process that results in the restoration of anatomical continuity and function. Albino Wistar rats (150-180 g) of both sexes were selected. The experiment of Wound Healing Activity by Excision Wound Model and Incision Wound Model by the simple ointment B.P., reference standard drug (0.2% w/w nitrofurazone ointment), stigmasterol ointment (0.2% w/w), hexane, chloroform and methanol extract ointments of 3%, 4% and 5% w/w of leaves in Jatropha tanjorensis in simple ointment base (where 3g, 4g and 5 g of the extracts was incorporated in 100 g of simple ointment separately). The time for wound closure to methanol extract ointment (5% w/w) and stigmasterol (0.2% w/w) was similar to that of standard drug, nitrofurazone ointment (0.2% w/w) 16 ± 2 days in Excision Wound Model. The significant tensile strength at 3%: 4%: 5% w/w methanol extract ointments (p<0.001), followed by chloroform extract and hexane extract. Stigmasterol ointment at 0.2% w/w produced tensile strength comparable with Standard drug, nitrofurazone ointment (0.2% w/w) (p<0.001) in Incision Wound Model.  Kirithikar and basu stated that the juice of the Jatropha plant and the pounded leaves are applied to wounds and refractory ulcers. The juice is very successfully used to treat scabies, eczema and ringworm. The present study proved that the leaves have wound healing activity.

scholarly journals Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness

2021 ◽  
Vol 12 ◽  
Author(s):  
Laryssa C. Manigat ◽  
Mitchell E. Granade ◽  
Suchet Taori ◽  
Charlotte Anne Miller ◽  
Luke R. Vass ◽  
...  
Keyword(s):  
Wound Healing ◽  
T Cell ◽  
Healing Process ◽  
Cell Activity ◽  
Regulatory Function ◽  
Burned Area ◽  
Wound Healing Process ◽  
Wound Model ◽  
Complex Wound

The diacylglycerol kinases (DGKs) are a family of enzymes responsible for the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). In addition to their primary function in lipid metabolism, DGKs have recently been identified as potential therapeutic targets in multiple cancers, including glioblastoma (GBM) and melanoma. Aside from its tumorigenic properties, DGKα is also a known promoter of T-cell anergy, supporting a role as a recently-recognized T cell checkpoint. In fact, the only significant phenotype previously observed in Dgka knockout (KO) mice is the enhancement of T-cell activity. Herein we reveal a novel, macrophage-specific, immune-regulatory function of DGKα. In bone marrow-derived macrophages (BMDMs) cultured from wild-type (WT) and KO mice, we observed increased responsiveness of KO macrophages to diverse stimuli that yield different phenotypes, including LPS, IL-4, and the chemoattractant MCP-1. Knockdown (KD) of Dgka in a murine macrophage cell line resulted in similar increased responsiveness. Demonstrating in vivo relevance, we observed significantly smaller wounds in Dgka-/- mice with full-thickness cutaneous burns, a complex wound healing process in which macrophages play a key role. The burned area also demonstrated increased numbers of macrophages. In a cortical stab wound model, Dgka-/- brains show increased Iba1+ cell numbers at the needle track versus that in WT brains. Taken together, these findings identify a novel immune-regulatory checkpoint function of DGKα in macrophages with potential implications for wound healing, cancer therapy, and other settings.

scholarly journals Investigating the Wound Healing Potential of the Polyherbal Gels Prepared with Ficus Extract

2018 ◽  
Vol 8 (2) ◽  
pp. 13-16
Author(s):  
Mahender K ◽  
Ravi D ◽  
Chaitanya Kumar K ◽  
Mothilal K
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Human Body ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Aqueous Extracts ◽  
Standard Drug ◽  
Carbopol Gel ◽  
Ficus Benghalensis

Wounds are nothing but any damage to the tissue or skin that can be healed. The wound healing process is usually built in the human body to self heal many wounds. When there is an injury in the body, there is an inflammatory response that is generated in the body, and the cells begin to raise the collagen levels in the skin which enables to increase the healing process. Ficus species of plants are famous for their potency to treat diseases in various Indian systems of medicine and the tree is commonly called as a banyan. Especially the plant in the species benghalensis is used to treat rheumatism, wounds and other skin related problems like an ulcer. The herbal gels were prepared using the incorporation of the aqueous extracts of the plant Ficus benghalensis into carbopol gel. They were investigated for the wound healing potential compared to the betadine drug standard. The gels at a concentration 200mg/g of the gel showed better activity compared to the gel at 100mg/g and the standard drug, betadine.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

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