scholarly journals Inhibitory Effects of Bangladeshi Medicinal Plant Extracts on Interactions between Transcription Factors and Target DNA Sequences

2008 ◽  
Vol 5 (3) ◽  
pp. 303-312 ◽  
Author(s):  
Ilaria Lampronti ◽  
Mahmud T. H. Khan ◽  
Monica Borgatti ◽  
Nicoletta Bianchi ◽  
Roberto Gambari
Keyword(s):  
Transcription Factors ◽  
Medicinal Plants ◽  
Cell Lines ◽  
Plant Extracts ◽  
Dna Sequences ◽  
Cell Cycle Progression ◽  
Biological Effects ◽  
Mobility Shift ◽  
Medicinal Plant Extracts ◽  
Target Dna

Several transcription factors (TFs) play crucial roles in governing the expression of different genes involved in the immune response, embryo or cell lineage development, cell apoptosis, cell cycle progression, oncogenesis, repair and fibrosis processes and inflammation. As far as inflammation, TFs playing pivotal roles are nuclear factor kappa B (NF-kB), activator protein (AP-1), signal transducer and activator of transcription (STATs), cAMP response element binding protein (CREB) and GATA-1 factors. All these TFs regulate the expression of pro-inflammatory cytokines and are involved in the pathogenesis of a number of human disorders, particularly those with an inflammatory component. Since several medicinal plants can be employed to produce extracts exhibiting biological effects and because alteration of gene transcription represents a very interesting approach to control the expression of selected genes, this study sought to verify the ability of several extracts derived from Bangladeshi medicinal plants in interfering with molecular interactions between different TFs and specific DNA sequences. We first analyzed the antiproliferative activity of 19 medicinal plants on different human cell lines, including erythroleukemia K562, B lymphoid Raji and T lymphoid Jurkat cell lines. Secondly, we employed the electrophoretic mobility shift assay as a suitable technique for a fast screening of plant extracts altering the binding between NF-kB, AP-1, GATA-1, STAT-3, CREB and the relative target DNA elements.

scholarly journals Antioxidant and antiproliferative activities on prostate and cervical cultured cancer cells of five medicinal plant extracts from Burkina Faso

2020 ◽  
Vol 14 (3) ◽  
pp. 652-663
Author(s):  
Bagora Bayala ◽  
Théodora Mahoukèdè Zohoncon ◽  
Florencia Wendkuuni Djigma ◽  
Christelle Nadembega ◽  
Silvère Baron ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Burkina Faso ◽  
Cell Lines ◽  
Plant Extracts ◽  
Antiproliferative Activity ◽  
Radical Scavenging ◽  
Euphorbia Hirta ◽  
Hela Cell Lines ◽  
Medicinal Plant Extracts ◽  
Antiproliferative Activities

Medicinal plants are a potential source of drug discovery and development of cancer chemoprevention drugs. Thus, the aim of this work was to study the antioxidant and antiproliferative activities of hydromethanolic extracts of Musa sapientum L., Cassia italica (Mill.) Spreng., Crateva adansonii DC., Euphorbia hirta L. and Ceratotheca sesamoides Endl. from Burkina Faso. The antioxidative activity of hydromethanolic extracts of plant was assessed using DPPH radical scavenging assay and ABTS+ radical cation decolorisation assay. Antiproliferative activity was evaluated by MTT assay. Of these five plant extracts, hydromethanolic extract of Euphorbia hirta leaf twigs showed the best antioxidant activity both by DPPH (IC50 = 0.53 ± 0.04 μg extract / μg DPPH) and ABTS (C = 0.302 ± 0.003 μMET / g extract) methods. In addition, hydromethanolic extract of Euphorbia hirta leaf twigs showed the best antiproliferative activity on LNCaP cell lines of prostate cancer while the hydromethanolic extract of the Ceratotheca sesamoides leaf stems showed the best antiproliferative activity on the HeLa cell lines of cervical cancer. This work has shown not only the antioxidant and anticancer activities of these five local plants, but also the potential valorization of these species used in traditional medicine in Burkina Faso.Keywords: Cancer, antioxydant, antiproliferative, Medicinal plants, Burkina Faso.

Screening of Venezuelan medicinal plant extracts for cytostatic and cytotoxic activity against tumour cell lines

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
P Taylor ◽  
M Arsenak ◽  
MJ Abad ◽  
Á Fernández ◽  
R Gonto ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Plant Extracts ◽  
Tumour Cell ◽  
Medicinal Plant Extracts ◽  
Tumour Cell Lines

scholarly journals Medicinal Plant Extracts

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 838
Author(s):  
Laura Grațiela Vicaș ◽  
Mariana Eugenia Mureșan
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Important Data ◽  
Therapeutic Benefits ◽  
Medicinal Plant Extracts

The therapeutic benefits of medicinal plants are well known and have been collected as important data on ethnomedicine [...]

scholarly journals TBP and SNAP50 transcription factors bind specifically to the Pr77 promoter sequence from trypanosomatid non-LTR retrotransposons

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Francisco Macías ◽  
Raquel Afonso-Lehmann ◽  
Patricia E. Carreira ◽  
M. Carmen Thomas
Keyword(s):  
Transcription Factors ◽  
Protein Interactions ◽  
Dna Sequences ◽  
Direct Interaction ◽  
Promoter Sequence ◽  
Nuclear Proteins ◽  
Hill Coefficient ◽  
Small Nuclear Rna ◽  
Mobile Dna ◽  
Mobility Shift

Abstract Background Trypanosomatid genomes are colonized by active and inactive mobile DNA elements, such as LINE, SINE-like, SIDER and DIRE retrotransposons. These elements all share a 77-nucleotide-long sequence at their 5′ ends, known as Pr77, which activates transcription, thereby generating abundant unspliced and translatable transcripts. However, transcription factors that mediates this process have still not been reported. Methods TATA-binding protein (TBP) and small nuclear RNA-activating protein 50 kDa (SNAP50) recombinant proteins and specific antibodies raised against them were generated. Protein capture assay, electrophoretic mobility-shift assays (EMSA) and EMSA competition assays carried out using these proteins and nuclear proteins of the parasite together to specific DNA sequences used as probes allowed detecting direct interaction of these transcription factors to Pr77 sequence. Results This study identified TBP and SNAP50 as part of the DNA-protein complex formed by the Pr77 promoter sequence and nuclear proteins of Trypanosoma cruzi. TBP establishes direct and specific contact with the Pr77 sequence, where the DPE and DPE downstream regions are docking sites with preferential binding. TBP binds cooperatively (Hill coefficient = 1.67) to Pr77 and to both strands of the Pr77 sequence, while the conformation of this highly structured sequence is not involved in TBP binding. Direct binding of SNAP50 to the Pr77 sequence is weak and may be mediated by protein–protein interactions through other trypanosomatid nuclear proteins. Conclusions Identification of the transcription factors that mediate Pr77 transcription may help to elucidate how these retrotransposons are mobilized within the trypanosomatid genomes and their roles in gene regulation processes in this human parasite. Graphic abstract

scholarly journals Chicken beta B1-crystallin gene expression: presence of conserved functional polyomavirus enhancer-like and octamer binding-like promoter elements found in non-lens genes.

1991 ◽  
Vol 11 (3) ◽  
pp. 1488-1499 ◽  
Author(s):  
H J Roth ◽  
G C Das ◽  
J Piatigorsky
Keyword(s):  
Transcription Factors ◽  
Hela Cell ◽  
Dna Sequences ◽  
Nuclear Extract ◽  
Regulatory Elements ◽  
Dnase I ◽  
Flanking Sequence ◽  
Mobility Shift ◽  
Promoter Elements ◽  
Dnase I Footprinting

Expression of the chicken beta B1-crystallin gene was examined. Northern (RNA) blot and primer extension analyses showed that while abundant in the lens, the beta B1 mRNA is absent from the liver, brain, heart, skeletal muscle, and fibroblasts of the chicken embryo, suggesting lens specificity. Promoter fragments ranging from 434 to 126 bp of 5'-flanking sequence (plus 30 bp of exon 1) of the beta B1 gene fused to the bacterial chloramphenicol acetyltransferase gene functioned much more efficiently in transfected embryonic chicken lens epithelial cells than in transfected primary muscle fibroblasts or HeLa cells. Transient expression of recombinant plasmids in cultured lens cells, DNase I footprinting, in vitro transcription in a HeLa cell extract, and gel mobility shift assays were used to identify putative functional promoter elements of the beta B1-crystallin gene. Sequence analysis revealed a number of potential regulatory elements between positions -126 and -53 of the beta B1 promoter, including two Sp1 sites, two octamer binding sequence-like sites (OL-1 and OL-2), and two polyomavirus enhancer-like sites (PL-1 and PL-2). Deletion and site-specific mutation experiments established the functional importance of PL-1 (-116 to -102), PL-2 (-90 to -76), and OL-2 (-75 to -68). DNase I footprinting using a lens or a HeLa cell nuclear extract and gel mobility shifts using a lens nuclear extract indicated the presence of putative lens transcription factors binding to these DNA sequences. Competition experiments provided evidence that PL-1 and PL-2 recognize the same or very similar factors, while OL-2 recognizes a different factor. Our data suggest that the same or closely related transcription factors found in many tissues are used for expression of the chicken beta B1-crystallin gene in the lens.

scholarly journals Medicinal plant extracts on the control of Diabrotica speciosa (Coleoptera: Chrysomelidae)

2013 ◽  
Vol 15 (1) ◽  
pp. 142-149 ◽  
Author(s):  
F.S. Barbosa ◽  
G.L.D. Leite ◽  
E.R. Martins ◽  
V.A. D'avila ◽  
V.M Cerqueira
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Soybean Oil ◽  
Plant Extracts ◽  
Chenopodium Ambrosioides ◽  
Insecticidal Effect ◽  
Medicinal Plant Extracts ◽  
Diabrotica Speciosa ◽  
Artemisia Verlotorum

The aim of this study was to evaluate the insecticidal effect of aqueous, alcoholic, and oil extracts from leaves of eight medicinal plants against Diabrotica speciosa prepared at five concentrations. The extracts that used commercial soybean oil as solvent showed the highest D. speciosa mortality due to the solvent itself, regardless of the used plants and their concentrations. Thus, commercial soybean oil was discarded as solvent since at these volumes it would cause serious phytotoxicity problems. After 24 hours of exposure of the pest to the extracts, the highest D. speciosa mortality values were observed for Copaifera langsdorfii and Chenopodium ambrosioides extracts, both in 5% alcohol, and Artemisia verlotorum, in 10% water. However, in the last mortality assessment (48 h), C. langsdorfii extract in 5% alcohol showed higher mortality of this pest, followed by C. ambrosioides extract in 5% alcohol, compared to the remaining plants.

scholarly journals BIO-CONTROL OF MULTIPLE DRUG-RESISTANT UROPATHOGENS USING MEDICINAL PLANT EXTRACTS

2019 ◽  
pp. 371-376
Author(s):  
VIGI CHAUDHARY ◽  
RAGHUVANSHI RK ◽  
NAVEEN CHAUDHARY ◽  
GAURAV SHARMA
Keyword(s):  
Urinary Tract ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Human Pathogens ◽  
Multiple Drug Resistant ◽  
Medicinal Plant Extracts ◽  
Tract Infections

Objective: The present study was conducted to evaluate the potential of some medicinal plants used in Ayurveda in treating multiple drug-resistant human pathogens causing urinary tract infections (UTIs). Methods: Dried parts of six medicinal plants used in Ayurveda for treating UTI were Soxhlet extracted, and the extract was concentrated in vacuo. Various concentrations of the extract were tested for antimicrobial activity against three clinical isolates of multiple drug-resistant bacteria causing UTI. Results: Preliminary results showed the promising antibacterial effect of plant extracts. Escherichia coli, the most common pathogen associated with UTI, was susceptible to aqueous extracts of all the six medicinal plants. Conclusion: This study concluded that the medicinal plants used in Ayurveda to treat UTIs are effective against multiple drug-resistant uropathogens. Further study in this regard may lead to the identification of novel antimicrobial agent for treating multiple drug-resistant urinary tract pathogens.

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