scholarly journals In VitroAnti-CandidaActivity ofZataria multifloraBoiss

2007 ◽  
Vol 4 (3) ◽  
pp. 351-353 ◽  
Author(s):  
Ali Zarei Mahmoudabadi ◽  
Muhammad Ali Dabbagh ◽  
Zahra Fouladi
Keyword(s):  
Medicinal Plants ◽  
Minimal Inhibitory Concentration ◽  
Candida Parapsilosis ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Zataria Multiflora ◽  
Aerial Parts ◽  
Ethanolic Extracts

Zataria multifloraBoiss known as Avishan Shirazi (in Iran) is one of the valuable Iranian medicinal plants. The aim of study was to evaluate anti-Candidaactivity ofZ. multifloraagainst different species ofCandida in vitro. Anti-Candidaactivity of the aqueous, ethanolic and methanolic maceration extract of the aerial parts ofZ. multiflora Boisswas studiedin vitro. Anti-Candidaactivity againstCandidaspecies was done using serial dilutions of extracts in Sabouraud's dextrose agar. Minimal inhibitory concentration (MIC) of the methanolic and ethanolic extracts was 70.7 and 127 mg l−1, respectively. Aqueous extract showed no remarkable activity againstCandidaspecies. We conclude that methanolic extract of the aerial parts ofZ. multifloraBoiss has more anti-Candidaeffect at 70.7 mg l−1compared to ethanolic extract 127 mg l−1. In addition, the isolates ofCandida parapsilosiswere more susceptible to methanolic extract than other tested species.

In-vitro Antibacterial Effects of Aqueous, Ethanolic, Methanolic, Acetone and Hydro Ethanolic Extracts of Achillea Millefolium on Standard Klebsiella, S. pyogenes, and Oral Bacterias Strains

2020 ◽  
Author(s):  
Negar Fallah ◽  
Sanaz Namazi ◽  
Negar Balmeh ◽  
Samira Mahmoudi ◽  
Fereshteh Mirzaei Poor
Keyword(s):  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Methanolic Extract ◽  
Growth Zone ◽  
Achillea Millefolium ◽  
Antibacterial Effects ◽  
Millipore Filter ◽  
Antimicrobial Effects ◽  
Aerial Parts

Introduction: So far, a lot of attempts have been carried out to find antimicrobial compounds. In this study, it was also tried to investigate the antibacterial effects of Achillea millefolium on standard Klebsiella, S. pyogenes, and oral bacterias strain. Material and Methods: The aerial parts of Achillea millefolium were used and the aqueous, ethanolic, methanolic, acetone and hydroethanolic extracts were prepared. After the preparation of standard strains of Klebsiella, S. pyogenes, and oral bacterias and sterilization of extracts by the Millipore filter, the antibacterial effects of these extracts on the mentioned microorganisms were assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and well diffusion at the concentration 50 mg/ml. The test was repeated three times for each bacterium. Results: It was exposed that aqueous extract of Achillea millefolium had the most distinguished antimicrobial effects against all studied strains and methanolic extract had antimicrobial effects only on S. pyogenes. MIC and MBC of effective extracts were the basic concentration (50mg/ml), and non-growth zone was not observed in other serial dilution in case of all bacteria. Conclusion: The Achillea millefolium can be admitted as an antibacterial medicinal herb. Thus, it can be concluded that after evaluating their effects in vitro, Achillea millefolium can be utilized as an alternative to the routine chemical drugs.

Exploring the potential effect of Methanolic extract of Salvia officinalis against UV exposed skin aging: In vivo and In vitro model

2019 ◽  
Vol 12 ◽  
Author(s):  
Ruchi Khare ◽  
Neeraj Upmanyu ◽  
Megha Jha
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Treated Group ◽  
Salvia Officinalis ◽  
Antioxidant Potential ◽  
Meoh Extract ◽  
Soxhlet Apparatus

Context: The medicinal plants have enormous pharmacological properties and having fewer side effects. Today there is increasing demand of medicinal plants as an anti-aging and anti-wrinkle agent. Objective: The aim of this study is to evaluate antioxidant, anti-aging and anti-wrinkle potential of Salvia officinalis. Materials and Methods: Salvia officinalis (Lamiaceae) is folk medicine of Asia and Latin America. Powdered crude drug 100 g were successively extracted in a soxhlet apparatus with petroleum ether (60-80ºC), chloroform and methanol. After successive solvents extraction methanolic extract was used for testing of antioxidant potential using DPPH assay. Further, antiaging potential of extract was investigated by inhibitory effect of various enzymatic estimations i.e. Col-I, Ela-I and Hya-I inhibitory assays on early aging human skin fibroblasts. Antiwrinkle potential of plant Salvia officinalis was done by using UV light induced photo aging model. Results: Phytochemical analysis showed the presence of glycosides, alkaloids flavonoids, and triterpenoids, saponins and Phenolic compounds in high level. Extract showed inhibitory concentration (IC50:24.65) and ascorbic acid the standard antioxidant showed inhibitory concentration (IC50:20.10). In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed showing inhibitory concentration as Col-I (IC50:21.36), Ela-I (IC50:35.05) and Hya-I (IC50:23.44) respectively. Thus, MeOH extract of Salvia officinalis able to inhibit 50% of the activity of aging related enzymes Col-I, Ela-I and Hya-I. The wrinkle score of negative control i.e. UV treated group was 2.83±0.408 and MeOH extract of Salvia officinalis treated group is 1.83 ±0.753. Conclusion: This study concluded that MeOH extract of Salvia officinalis has confirmed the high antioxidant potential and In vitro and In vivo inhibitory potential of antiaging enzymes assessed, thus they could be used for further development of cosmetic products and nutraceuticals.

scholarly journals Uji Aktivitas Ekstrak Daun Mengkudu (Morindra citrifolia Linn) dan Scopoletin secara In-Vitro terhadap Bakteri Tuberkulosis

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Estu Mahanani Dhilasari ◽  
Idha Kusumawati Kusumawati ◽  
Riesta Primaharinastiti Primaharinastiti
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Mycobacterium Tuberculosis H37rv ◽  
Ethanolic Extracts ◽  
Tuberculosis Activity ◽  
Strain H37rv ◽  
Mycobacterium Tuberculosis Strain

Penyakit tuberkulosis disebabkan oleh bakteri Mycobacterium tuberculosis. Secara tradisional mengkudu digunakan untuk pengobatan tuberkulosis. Scopoletin merupakan komponen utama dalam mengkudu, oleh karena itu scopoletin sering dijadikan marker dalam studi farmakokinetik. Tujuan penelitian ini adalah untuk mengetahui aktivitas anti-Mycobacterium tuberculosis (H37RV) ekstrak daun mengkudu dan scopoletin melalui penentuan Konsentrasi Hambat Minimum (KHM). Uji aktivitas antibakteri dan penentuan KHM dari ekstrak etanol 50% daun mengkudu dilakukan dengan metode dilusi agar dengan konsentrasi 1,0×10-4 µg/ml – 5,1×10-11 µg/ml. Uji aktivitas menunjukan bahwa ekstrak etanol 50% daun mengkudu dapat menghambat pertumbuhan Mycobacterium tuberculosis dengan KHM 4,0×10-6 µg/ml. Sedangkan scopoletin dengan konsentrasi yang setara dengan kandungan pada ekstrak tidak menunjukan aktivitas anti-Mycobacterium tuberculosisnya.Kata kunci: Mengkudu, Scopoletin, tuberkulosisTuberculosis (TB) is a disease caused by a bacterium, Mycobacterium tuberculosis. Morinda citrifolia Linn has been found to kill Mycobacterium tuberculosis. Scopoletin is a major component in Morinda citrifolia Linn, therefore scopoletin often used as markers in studies of pharmacokinetic. This research purpose to determine anti-Mycobacterium tuberculosis (strain H37RV) activity based on the value of Minimum Inhibitory Concentration (MIC) used to ethanolic extracts from Morinda citrifolia Linn leaf and scopoletin. Experiment of anti-Mycobacterium tuberculosis activities tested by well dillution methods with a dose 1,0×10-4 µg/ml – 5,1×10-11 µg/ml . The results showed that ethanolic extract Morinda citrifolia Linn leaf  were found to be active to Mycobacterium tuberculosis activity with MIC 4×10-6 µg/ml while scopoletin at the same concentration with extract had no anti­- Mycobacterium tuberculosis activity.Keywords:  Noni, scopoletin, tuberculosis

scholarly journals Phytochemical analysis and antioxidant activity of some thymus species from Romania

2021 ◽  
Vol 14 (1) ◽  
pp. 27-33
Author(s):  
Florina Căpățină ◽  
◽  
Eva Suciu ◽  
Daniela Benedec ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Analysis ◽  
Chromatography Method ◽  
Aerial Parts ◽  
Polyphenolic Composition ◽  
Dpph Method ◽  
Ethanolic Extracts ◽  
Layer Chromatography

Objectives. Thymus species, T. vulgaris, T. serpyllum, T. comosus and T. glabrescens, are medicinal plants from the spontaneous Romanian flora used for their antibacterial, antispasmodic, expectorant, anti-inflammatory, anthelmintic properties, especially the plant products obtained from T. vulgaris. The aim of this study was to comparatively investigate the polyphenolic composition, as well as the in vitro antioxidant activity of some ethanolic extracts obtained from the aerial parts of thymus sp. Materials and methods. The polyphenols, flavonoids, phenolic acids and tannins contents in the four samples thymus sp. ethanolic extracts were spectrophotometrically determined. The identification of the most important polyphenolic compounds was performed by the method of thin layer chromatography method (TLC). The antioxidant activity of hydroalcoholic extracts was evaluated in vitro by the DPPH method. Outcomes. Following the analyzes performed, it can be revealed that there are both qualitative and quantitative differences in the polyphenolic composition of the four species of thyme studied, the richest in active principles being T. vulgaris and T. serpyllum. Conclusions. In this paper it has been shown that thyme species are medicinal plants rich in antioxidant polyphenolic active principles, and their use for the treatment of certain diseases can be justified. In addition to the officinal species: T. vulgaris, T. serpyllum, other thyme species from the spontaneous flora of our country can be used in phytotherapy, such as: T. comosus, T. glabrescens.

α-Glucosidase Inhibition and Docking Studies of 5-Deoxyflavonols and Dihydroflavonols Isolated from Abutilon pakistanicum

2019 ◽  
Vol 23 (17) ◽  
pp. 1857-1866
Author(s):  
Munawar Hussain ◽  
Zaheer Ahmed ◽  
Shamsun N. Khan ◽  
Syed A. A. Shah ◽  
Rizwana Razi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Docking Studies ◽  
Hydroxyl Group ◽  
Coumaric Acid ◽  
In Vitro Activity ◽  
Aerial Parts ◽  
First Time ◽  
Acid Esters ◽  
Phenol Moiety

Three new 5-deoxyflavonoid and dihydroflavonoids 2, 3 and 4 have been isolated from the methanolic extract of Abutioln pakistanicum aerial parts, for which structures were elucidated explicitly by extensive MS- and NMR-experiments. In addition to these, 3,7,4′-trihydroxy-3′-methoxy flavonol (1) is reported for the first time from Abutioln pakistanicum. Compound 2 and 4 are p-coumaric acid esters while compounds 2–4 exhibited α-glucosidase inhibitory activity. Docking studies indicated that the ability of flavonoids 2, 3 and 4 to form multiple hydrogen bonds with catalytically important residues is decisive hence is responsible for the inhibition activity. The docking results signified the observed in-vitro activity quite well which is in accordance with previously obtained conclusion that phenol moiety and hydroxyl group are critical for the inhibition of α-glucosidase enzyme.

In Vitro Antibacterial activity of ethanolic extract of Myrsine africana against laboratory Strains of Pathogenic Organisms

2016 ◽  
Vol 5 (04) ◽  
pp. 4512
Author(s):  
Jackie K. Obey ◽  
Anthoney Swamy T* ◽  
Lasiti Timothy ◽  
Makani Rachel
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
E Coli ◽  
Zones Of Inhibition ◽  
In Vitro Antibacterial Activity ◽  
Myrsine Africana

The determination of the antibacterial activity (zone of inhibition) and minimum inhibitory concentration of medicinal plants a crucial step in drug development. In this study, the antibacterial activity and minimum inhibitory concentration of the ethanol extract of Myrsine africana were determined for Escherichia coli, Bacillus cereus, Staphylococcus epidermidis and Streptococcus pneumoniae. The zones of inhibition (mm±S.E) of 500mg/ml of M. africana ethanol extract were 22.00± 0.00 for E. coli,20.33 ±0.33 for B. cereus,25.00± 0.00 for S. epidermidis and 18. 17±0.17 for S. pneumoniae. The minimum inhibitory concentration(MIC) is the minimum dose required to inhibit growth a microorganism. Upon further double dilution of the 500mg/ml of M. africana extract, MIC was obtained for each organism. The MIC for E. coli, B. cereus, S. epidermidis and S. pneumoniae were 7.81mg/ml, 7.81mg/ml, 15.63mg/ml and 15.63mg/ml respectively. Crude extracts are considered active when they inhibit microorganisms with zones of inhibition of 8mm and above. Therefore, this study has shown that the ethanol extract of M. africana can control the growth of the four organisms tested.

scholarly journals Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeerati Prompipak ◽  
Thanaset Senawong ◽  
Banchob Sripa ◽  
Albert J. Ketterman ◽  
Suppawit Utaiwat ◽  
...  
Keyword(s):  
Nude Mice ◽  
Combination Treatment ◽  
Synergistic Effects ◽  
Single Agent ◽  
Xenograft Model ◽  
Ethanolic Extract ◽  
Model Combination ◽  
Mouse Xenograft Model ◽  
Ethanolic Extracts

AbstractApplication of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy of 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim of this study was to evaluate the combination effects of 5-FU and two ethanolic extracts of Thai noni juice (TNJ) products on CCA cell lines and nude mice xenografts. The results of antiproliferative assay showed the combination treatment of 5-FU and each TNJ ethanolic extract exerted more cytotoxicity on CCA cells than either single agent treatment. Synergistic effects of drug combinations can enable the dose reduction of 5-FU. The mechanism underlying a combination treatment was apoptosis induction through an activation of p53 and Bax proteins. In the nude mouse xenograft model, combination treatments of 5-FU with each TNJ ethanolic extract suppressed the growth of CCA cells implanted mice more than single agent treatments with no effects on mouse body weight, kidney, and spleen. Moreover, low doses of TNJ ethanolic extracts reduced the hepatotoxicity of 5-FU in nude mice. Taken together, these data suggested that the ethanolic extracts of TNJ products can enhance the anti-CCA effect and reduce toxicity of 5-FU.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii)

2016 ◽  
Vol 16 (1) ◽  
pp. 9
Author(s):  
Ummu Balqis ◽  
Darmawi Darmawi ◽  
Maryam Maryam ◽  
Muslina Muslina ◽  
Abdullah Hamzah ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
The Body ◽  
Whole Body ◽  
Ascaridia Galli ◽  
Fourth Group ◽  
The Third ◽  
Ethanolic Extracts ◽  
Aqueous Ethanolic Extract ◽  
Normal Controls

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.  (Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii) ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.

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