scholarly journals Assay validation and interspecific comparison of salivary glucocorticoids in three amphibian species

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Talisin T Hammond ◽  
Zoe A Au ◽  
Allison C Hartman ◽  
Corinne L Richards-Zawacki
Keyword(s):  
Interspecific Comparison ◽  
Amphibian Species ◽  
Assay Validation

Cryobanking of Amphibian Genetic Recourses in Russia: Past and Future

2019 ◽  
Vol 26 (6) ◽  
pp. 319-324
Author(s):  
Victor K. Uteshev ◽  
Edith N. Gakhova ◽  
Ludmila I. Kramarova ◽  
Natalia V. Shishova ◽  
Svetlana A. Kaurova ◽  
...  
Keyword(s):  
Genetic Material ◽  
Reproductive Technologies ◽  
Cell Biophysics ◽  
Amphibian Species ◽  
Modern Biology ◽  
Rare And Endangered Species ◽  
Long Term Storage ◽  
Current State ◽  
Rare And Endangered ◽  
Academy Of Sciences

In modern biology, a search for efficient and safe ways of long-term storage of animal genomes is vital for the survival of rare and endangered species. To date, the only reliable method of prolonging the preservation of genome is deep freezing of somatic and reproductive cells, including spermatozoa. Here we overview the current state of reproductive technologies and present the cryopresevation strategies of genetic material of selected amphibian species. These strategies were developed at the Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia. Cryopreservation could help managing the threatened populations of amphibian species through genome storage and mediate the production of healthy animals from the stored material.

scholarly journals An Overview of the Helminths of Moor Frog Rana arvalis Nilsson, 1842 (Amphibia: Anura) in the Volga Basin

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 61
Author(s):  
Igor V. Chikhlyaev ◽  
Alexander B. Ruchin
Keyword(s):  
Systematic Position ◽  
Helminth Fauna ◽  
Helminth Species ◽  
Volga Basin ◽  
Rana Arvalis ◽  
Amphibian Species ◽  
Volga River ◽  
Spirometra Erinacei ◽  
First Time ◽  
Moor Frog

This is the first review of the helminth fauna of the moor frog Rana arvalis Nilsson, 1842 from the Volga river basin (Russia). The article summarizes the authors’ and literature data on the helminthic fauna of this species. The method of complete helminthological dissection was used. Thirthy-eight helminth species were recorded from three classes: Cestoda (1), Trematoda (28), and Chromadorea (9). Nine helminth species are new to the moor frog in Russia: trematodes Gorgodera varsoviensis Sinitzin, 1905, Strigea falconis Szidat, 1928, larvae, Neodiplostomum spathoides Dubois, 1937, larvae, Tylodelphys excavata (Rudolphi, 1803), larvae, Pharyngostomum cordatum (Diesing, 1850), larvae, Astiotrema monticelli Stossich, 1904, larvae and Encyclometra colubrimurorum (Rudolphi, 1819), larvae, nematodes Strongyloides spiralis Grabda-Kazubska, 1978 and Icosiella neglecta (Diesing, 1851). The cestode Spirometra erinacei (Rudolphi, 1918), larvae were observed of this amphibian species in the Volga basin for the first time. The nematodes Rhabdias bufonis, Oswaldocruzia filiformis, Cosmocerca ornata and the trematode Haplometra cylindracea form the core of the helminth fauna of the moor frog. Information on species of helminths includes systematic position, localization, areas of detection, type and scheme of life cycle, geographical distribution, and degree of specificity to host amphibians.

scholarly journals Synergistic impacts of global warming and thermohaline circulation collapse on amphibians

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Julián A. Velasco ◽  
Francisco Estrada ◽  
Oscar Calderón-Bustamante ◽  
Didier Swingedouw ◽  
Carolina Ureta ◽  
...  
Keyword(s):  
Climate Change ◽  
Global Warming ◽  
Thermohaline Circulation ◽  
Climate Model ◽  
Global Climate ◽  
Extinction Risk ◽  
Meridional Overturning Circulation ◽  
Mitigation Strategies ◽  
Amphibian Species ◽  
Climate Conditions

AbstractImpacts on ecosystems and biodiversity are a prominent area of research in climate change. However, little is known about the effects of abrupt climate change and climate catastrophes on them. The probability of occurrence of such events is largely unknown but the associated risks could be large enough to influence global climate policy. Amphibians are indicators of ecosystems’ health and particularly sensitive to novel climate conditions. Using state-of-the-art climate model simulations, we present a global assessment of the effects of unabated global warming and a collapse of the Atlantic meridional overturning circulation (AMOC) on the distribution of 2509 amphibian species across six biogeographical realms and extinction risk categories. Global warming impacts are severe and strongly enhanced by additional and substantial AMOC weakening, showing tipping point behavior for many amphibian species. Further declines in climatically suitable areas are projected across multiple clades, and biogeographical regions. Species loss in regional assemblages is extensive across regions, with Neotropical, Nearctic and Palearctic regions being most affected. Results underline the need to expand existing knowledge about the consequences of climate catastrophes on human and natural systems to properly assess the risks of unabated warming and the benefits of active mitigation strategies.

scholarly journals A rapid point-of-care assay accurately measures vitamin D

2021 ◽  
Author(s):  
K. Albrecht ◽  
J. Lotz ◽  
L. Frommer ◽  
K. J. Lackner ◽  
G. J. Kahaly
Keyword(s):  
Vitamin D ◽  
Metabolic Pathways ◽  
Point Of Care ◽  
Immunofluorescence Assay ◽  
Laboratory Method ◽  
Controlled Study ◽  
Mean Deviation ◽  
Serum Samples ◽  
Assay Validation ◽  
Accuracy And Precision

Abstract Purpose Vitamin D (VitD) is a pleiotropic hormone with effects on a multitude of systems and metabolic pathways. Consequently, the relevance of a sufficiently high VitD serum level becomes self-evident. Methods A rapid immunofluorescence assay designed for the point-of-care measurement of serum VitD3 solely was tested. Inter- and intra-assay validation, double testing and result comparison with a standardized laboratory method were performed. Results An overall linear correlation of r = 0.89 (Pearson, 95% CI 0.88–0.92, p < 0.01) between the point of care and the conventional reference assay was registered. Accuracy and precision were of special interest at cut-points (10 ng/ml [mean deviation 1.7 ng/ml, SD 1.98 ng/ml, SE 0.16 ng/ml], 12 ng/ml [MD 0.41, SD 1.89, SE 0.19] and 30 ng/ml [MD − 1.11, SD 3.89, SE 0.35]). Only a slight deviation was detected between the two assays when using fresh (r = 0.91, 95% CI 0.86–0.94, p < 0.01) and frozen serum samples (r = 0.86, 0.82–0.89, p < 0.01). Results remained steady when samples were frozen several times. Inter- and intra-assay validation according to the CLSI protocol as well as multiuser testing showed stable results. Conclusion This novel, innovative, and controlled study indicates that the evaluated rapid point of care VitD assay is reliable, accurate, and suited for clinical practice.

Dietary ecology of common amphibian species in a seasonal location in northern Sri Lanka

2020 ◽  
Vol 54 (27-28) ◽  
pp. 1797-1812
Author(s):  
R. B. Loshitha R. Sarathchandra ◽  
Kanishka D. B. Ukuwela ◽  
Rajnish P. G. Vandercone
Keyword(s):  
Sri Lanka ◽  
Amphibian Species ◽  
Dietary Ecology

scholarly journals Examining the Evidence for Chytridiomycosis in Threatened Amphibian Species

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23150 ◽  
Author(s):  
Matthew Heard ◽  
Katherine F. Smith ◽  
Kelsey Ripp
Keyword(s):  
Amphibian Species

Relationships among Isolated Wetland Size, Hydroperiod, and Amphibian Species Richness: Implications for Wetland Regulations

2000 ◽  
Vol 14 (2) ◽  
pp. 414-419 ◽  
Author(s):  
Joel W. Snodgrass ◽  
Mark J. Komoroski ◽  
A. Lawrence Bryan ◽  
Joanna Burger
Keyword(s):  
Species Richness ◽  
Amphibian Species ◽  
Isolated Wetland

scholarly journals P115 BONE ALKALINE PHOSPHATASE (BAP) ASSAY VALIDATION USING EQUINE SERUM AND SYNOVIAL FLUID

2006 ◽  
Vol 14 ◽  
pp. S73-S74
Author(s):  
T.N. Trumble ◽  
M.P. Brown ◽  
K.A. Merritt ◽  
R.C. Billinghurst
Keyword(s):  
Alkaline Phosphatase ◽  
Synovial Fluid ◽  
Bone Alkaline Phosphatase ◽  
Assay Validation

Widespread infection of the Eastern red-spotted newt (Notophthalmus viridescens) by a new species of Amphibiocystidium, a genus of fungus-like mesomycetozoan parasites not previously reported in North America

Parasitology ◽  
2007 ◽  
Vol 135 (2) ◽  
pp. 203-215 ◽  
Author(s):  
T. R. RAFFEL ◽  
T. BOMMARITO ◽  
D. S. BARRY ◽  
S. M. WITIAK ◽  
L. A. SHACKELTON
Keyword(s):  
New Species ◽  
North America ◽  
Infectious Diseases ◽  
Emerging Infectious Diseases ◽  
Seasonal Distribution ◽  
Notophthalmus Viridescens ◽  
Amphibian Species ◽  
Mortality And Morbidity ◽  
Causative Agents ◽  
A New Species

SUMMARYGiven the worldwide decline of amphibian populations due to emerging infectious diseases, it is imperative that we identify and address the causative agents. Many of the pathogens recently implicated in amphibian mortality and morbidity have been fungal or members of a poorly understood group of fungus-like protists, the mesomycetozoans. One mesomycetozoan, Amphibiocystidium ranae, is known to infect several European amphibian species and was associated with a recent decline of frogs in Italy. Here we present the first report of an Amphibiocystidium sp. in a North American amphibian, the Eastern red-spotted newt (Notophthalmus viridescens), and characterize it as the new species A. viridescens in the order Dermocystida based on morphological, geographical and phylogenetic evidence. We also describe the widespread and seasonal distribution of this parasite in red-spotted newt populations and provide evidence of mortality due to infection.

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