scholarly journals Galactomannan, β-D-Glucan, and Polymerase Chain Reaction–Based Assays for the Diagnosis of Invasive Fungal Disease in Pediatric Cancer and Hematopoietic Stem Cell Transplantation: A Systematic Review and Meta-Analysis

2016 ◽  
Vol 63 (10) ◽  
pp. 1340-1348 ◽  
Author(s):  
Thomas Lehrnbecher ◽  
Paula D. Robinson ◽  
Brian T. Fisher ◽  
Elio Castagnola ◽  
Andreas H. Groll ◽  
...  
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Pediatric Cancer ◽  
Meta Analysis ◽  
Invasive Fungal Disease ◽  
Chain Reaction ◽  
Hematopoietic Stem ◽  
Polymerase Chain

scholarly journals Comparison of serology, antigenemia assay and the polymerase chain reaction for monitoring active cytomegalovirus infections in hematopoietic stem cell transplantation patients

2006 ◽  
Vol 48 (5) ◽  
pp. 275-278 ◽  
Author(s):  
Sandra Helena Alves Bonon ◽  
Cláudio Lúcio Rossi ◽  
Cármino Antonio de Souza ◽  
Afonso Celso Vigorito ◽  
Sandra Cecília Botelho Costa
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Median Time ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Hematopoietic Stem ◽  
Antigenemia Assay ◽  
Polymerase Chain

Forty-six allogeneic hematopoietic stem cell transplantation (HSCT) patients were monitored for the presence of CMV antibodies, CMV-DNA and CMV antigens after transplantation. Immunoenzymatic serological tests were used to detect IgM and the increase in CMV IgG antibodies (increase IgG), a nested polymerase chain reaction (N-PCR) was used to detect CMV-DNA, and an antigenemia assay (AGM) was used to detect CMV antigens. The presence of CMV-IgM and/or CMV-increase IgG antibodies was detected in 12/46 (26.1%) patients, with a median time between HSCT and the detection of positive serology of 81.5 days. A positive AGM was detected in 24/46 (52.2%) patients, with a median time between HSCT and antigen detection of 62 days. Two or more consecutive positive N-PCR results were detected in 32/46 (69.5%) patients, with a median time between HSCT and the first positive PCR of 50.5 days. These results confirmed that AGM and mainly PCR are superior to serology for the early diagnosis of CMV infection. Six patients had CMV-IgM and/or CMV-increase IgG with a negative AGM (five cases) or N-PCR assay (one case). In five of these cases the serological markers were detected during the first 100 days after HSCT, the period of highest risk. These findings support the idea that serology may be useful for monitoring CMV infections in HSCT patients, especially when PCR is unavailable.

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