scholarly journals Performance of Candida Real-time Polymerase Chain Reaction,  -D-Glucan Assay, and Blood Cultures in the Diagnosis of Invasive Candidiasis

2012 ◽  
Vol 54 (9) ◽  
pp. 1240-1248 ◽  
Author(s):  
M. H. Nguyen ◽  
M. C. Wissel ◽  
R. K. Shields ◽  
M. A. Salomoni ◽  
B. Hao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Invasive Candidiasis ◽  
Blood Cultures ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Polymerase Chain Reaction-And Rna Hybridization-Based Method for the Investigation of Deep-Seated Candidiasis

1997 ◽  
Vol 8 (6) ◽  
pp. 329-334 ◽  
Author(s):  
Peter R Couroux ◽  
Zafar Hussain ◽  
Frank Rutledge ◽  
Robert Lannigan ◽  
Edward D Ralph ◽  
...  
Keyword(s):  
Risk Factors ◽  
Polymerase Chain Reaction ◽  
Invasive Candidiasis ◽  
Gel Electrophoresis ◽  
Tertiary Care ◽  
Immunosuppressive Drugs ◽  
Blood Cultures ◽  
Hybridization Method ◽  
Chain Reaction ◽  
Polymerase Chain

OBJECTIVE: To determine the usefulness of a polymerase chain reaction (PCR) and RNA hybridization method for the diagnosis of invasive candidiasis and to compare its sensitivity with blood cultures.DESIGN: Blood cultures and a blood sample for PCR were taken from patients with suspected invasive candidiasis. A 105 base pair conserved segment within the rDNA ofCandidaspecies was amplified. The amplicon was detected by hybridization and gel electrophoresis.SETTING: Intensive care units of two tertiary care hospitals.PATIENTS: One hundred and eighteen patients 16 years of age or older with four more risk factors for invasive candidiasis were enrolled. Present or recent past treatment with broad spectrum antibiotics, cancer chemotherapy, immunosuppressive drugs, granulocytopenia or granulocytosis, intravascular catheterization, tracheal intubation, recent abdominal surgery and parenteral nutrition were considered risk factors.RESULTS: Forty-three patients had invasive candidiasis. PCR detected infections in 28 and 26 patients (sensitivity 65.1% and 60.4%) by hybridization and gel electrophoresis, respectively. The sensitivity of blood cultures was 58.1%. Of 25 patients with positive blood cultures, 17 were positive by PCR with the hybridization method. Eleven patients with invasive candidiasis had negative blood cultures but were positive by PCR.CONCLUSION: PCR, especially with a hybridization detection method, is more sensitive than blood culture for invasive candidiasis and may facilitate the diagnosis of nonfungemic disease.

Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction

2010 ◽  
Vol 66 (2) ◽  
pp. 204-206 ◽  
Author(s):  
Laura Selva ◽  
Cristina Esteva ◽  
Amadeu Gené ◽  
Maria Fernández de Sevilla ◽  
Susanna Hernandez-Bou ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Pneumoniae ◽  
Real Time ◽  
Direct Detection ◽  
Blood Cultures ◽  
Chain Reaction ◽  
Polymerase Chain

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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