scholarly journals Evaluation of In-house Genotyping Assay Performance Using Dried Blood Spot Specimens in the Global World Health Organization Laboratory Network

2012 ◽  
Vol 54 (suppl 4) ◽  
pp. S273-S279 ◽  
Author(s):  
N. Parkin ◽  
C. de Mendoza ◽  
R. Schuurman ◽  
C. Jennings ◽  
J. Bremer ◽  
...  
Keyword(s):  
World Health Organization ◽  
Dried Blood Spot ◽  
World Health ◽  
Genotyping Assay ◽  
Laboratory Network ◽  
Assay Performance ◽  
Health Organization ◽  
Global World ◽  
Blood Spot

Using dried blood spot testing for diagnosing viral hepatitis

2020 ◽  
Vol 29 (20) ◽  
pp. 1155-1158
Author(s):  
Kathryn Jack ◽  
William Lucien Irving
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Public Health Problem ◽  
Dried Blood Spot ◽  
World Health ◽  
Populations At Risk ◽  
Health Organization ◽  
Sample Method ◽  
Drug And Alcohol ◽  
Blood Spot

The target set by the World Health Organization to eliminate viral hepatitis as a public health problem by 2030 first requires methods of testing for hepatitis B and C virus that are acceptable to diverse populations. One such test is the dried blood spot sample method. This article explains what a dried blood spot sample is, how it is collected, and how it can help increase the viral hepatitis test uptake in prisons, drug and alcohol services, and other populations at risk of hepatitis B or C infection.

scholarly journals Monkey experiments provide confirmation that a novel coronavirus is the cause of severe acute respiratory syndrome (SARS)

2003 ◽  
Vol 7 (16) ◽  
Author(s):  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
World Health Organization ◽  
Diagnostic Test ◽  
World Health ◽  
Laboratory Network ◽  
The World ◽  
Expert Meeting ◽  
Novel Coronavirus ◽  
Health Organization

The World Health Organization (WHO) has formally announced that a member of the coronavirus family never before seen in humans, named by WHO and its SARS laboratory network as the SARS virus, is the cause of severe acute respiratory syndrome (SARS) (1). An expert meeting in Geneva, attended by representatives from the SARS laboratory network, reviewed the data available on SARS, and considered the imminent strategy for development of a diagnostic test for SARS.

scholarly journals PENGARUH KONSENTRASI KENTOS KELAPA TERHADAP DEGRADASI LEMAK DAGING AYAM

2019 ◽  
pp. 38-43
Author(s):  
Andi Nur Fajri Suloi ◽  
Laras Budyghifari ◽  
Sitti Syuhada Dwi Arista ◽  
Amran Laga
Keyword(s):  
World Health Organization ◽  
World Health ◽  
Health Organization ◽  
Global World

Penyakit kardiovaskular seperti hipertensi, jantung koroner, gagal jantung, dan stroke menyebabkan 17,9 juta orang meninggal dunia atau 31% dari seluruh kematian global (World Health Organization, 2017). Penyakit ini pada umumnya diakibatkan oleh tingginya konsumsi pangan sumber lemak. Daging ayam bagian paha merupakan salah satu bahan pangan berlemak tinggi (7%) dibandingkan dengan potongan daging sapi (6,8%) dan daging domba (4,9-5,2%) dengan tingkat konsumsi yang tinggi (Dinh et al., 2011). Ekstrak kasar enzim lipase secara efisien dapat menghidrolisis lemak menjadi asam lemak bebas dan gliserol. Penelitian ini dilakukan untuk melihat pengaruh konsentrasi kentos kelapa sebagai sumber enzim lipase pada hidrolisis lemak daging ayam bagian paha. Kentos kelapa dipreparasi lalu diaplikasikan pada daging ayam dengan konsentrasi 1-5% dan 10-50%. Parameter pengamatan meliputi perubahan pH, Total Padatan Terlarut, dan Asam Lemak Bebas. Hasil terbaik untuk tiap parameter ialah perlakukan dengan konsentrasi kentos sebesar 10% dengan asam lemak bebas sebesar 2.99%, total padatan terlarut sebesar 4,1% dan pH sebesar 5.3

scholarly journals Evaluation of Two Techniques for Viral Load Monitoring Using Dried Blood Spot in Routine Practice in Vietnam (French National Agency for AIDS and Hepatitis Research 12338)

2016 ◽  
Vol 3 (3) ◽  
Author(s):  
Fabien Taieb ◽  
Tran Hong Tram ◽  
Hien Thi Ho ◽  
Van Anh Pham ◽  
Huong Lan Nguyen ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spot ◽  
World Health ◽  
Hiv Care ◽  
Routine Practice ◽  
National Agency ◽  
Resource Limited ◽  
Load Monitoring ◽  
Health Organization ◽  
Blood Spot

Abstract Background.  Although it is the best method to detect early therapeutic failure, viral load (VL) monitoring is still not widely available in many resource-limited settings because of difficulties in specimen transfer, personnel shortage, and insufficient laboratory infrastructures. Dried blood spot (DBS) use, which was introduced in the latest World Health Organization recommendations, can overcome these difficulties. This evaluation aimed at validating VL measurement in DBS, in a laboratory without previous DBS experience and in routine testing conditions. Methods.  Human immunodeficiency virus (HIV)-infected adults were observed in a HIV care site in Hanoi, and each patient provided 2 DBS cards with whole blood spots and 2 plasma samples. Viral load was measured in DBS and in plasma using the COBAS Ampliprep/TaqMan and the Abbott RealTime assays. To correctly identify those with VL ≥ 1000 copies/mL, sensitivity and specificity were estimated. Results.  A total of 198 patients were enrolled. With the Roche technique, 51 plasma VL were ≥1000 copies/mL; among these, 28 presented a VL in DBS that was also ≥1000 copies/mL (sensitivity, 54.9; 95% confidence interval [CI], 40.3–68.9). On the other hand, all plasma VL < 1000 copies/mL were also <1000 copies/mL in DBS (specificity, 100; 95% CI, 97.5–100). With the Abbott technique, 45 plasma VL were ≥1000 copies/mL; among these, 42 VL in DBS were also ≥1000 copies/mL (sensitivity, 93.3%; 95% CI, 81.7–98.6); specificity was 94.8 (95% CI, 90.0–97.7). Conclusions.  The Abbott RealTime polymerase chain reaction assay provided adequate VL results in DBS, thus allowing DBS use for VL monitoring.

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