scholarly journals Non-Anthocyanin Phenolic Compounds in Açaí (Euterpe oleraceaMart.) Juice by Ultrahigh-Performance Liquid Chromatography-Diode Array Detector (UHPLC-DAD): A Multivariate Optimization

2018 ◽  
Vol 57 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Tayse Ferreira Ferreira da Silveira ◽  
Helena Teixeira Godoy
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Array Detector ◽  
Diode Array ◽  
Multivariate Optimization ◽  
Diode Array Detector ◽  
Ultrahigh Performance Liquid Chromatography

scholarly journals Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

scholarly journals Clitorienolactones and Isoflavonoids of Clitorea ternatea Roots Alleviate Stress-Like Symptoms in a Reserpine-Induced Zebrafish Model

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4137
Author(s):  
Muhammad Afiq Ngadni ◽  
Muhammad Tayyab Akhtar ◽  
Intan Safinar Ismail ◽  
Anis Irfan Norazhar ◽  
Soo Yee Lee ◽  
...  
Keyword(s):  
Mental Illnesses ◽  
Array Detector ◽  
Diode Array ◽  
Root Extract ◽  
Diode Array Detector ◽  
Aqueous Methanol ◽  
Zebrafish Model ◽  
Metabolite Profiles ◽  
Ultrahigh Performance Liquid Chromatography ◽  
As Stress

Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated using ultrahigh-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC–DAD–MS/MS), while their effect on the stress-like behavior of zebrafish, pharmacologically induced with reserpine, was investigated. A total of 32 compounds were putatively identified, among which, a series of norneolignans, clitorienolactones, and various flavonoids (flavone, flavonol, isoflavone, and isoflavanone) was found to comprise the major constituents, particularly in the EA and 50% MeOH fractions. The clitorienolactones, presently unique to the species, were present in both the free and glycosylated forms in the roots. Both the EA and 50% MeOH fractions displayed moderate effects on the stress-induced zebrafish model, significantly decreasing freezing duration and elevating the total distance travelled and average velocity, 72 h post-treatment. The results of the present study provide further evidence that the basis for the use of C. ternatea roots in traditional medicine to alleviate brain-related conditions, such as stress and depression, is attributable to the presence of clitorienolactones and the isoflavonoidal constituents.

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