scholarly journals Simultaneous Determination of Valproic Acid and Its Major Metabolites by UHPLC-MS/MS in Chinese Patients: Application to Therapeutic Drug Monitoring

2016 ◽  
Author(s):  
Mingming Zhao ◽  
Ti Zhang ◽  
Guofei Li ◽  
Feng Qiu ◽  
Yaxin Sun ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Drug Monitoring ◽  
Chinese Patients ◽  
Therapeutic Drug

LC-MS for Simultaneous Determination of Vancomycin and Teicoplanin in Patient Plasma and its Application to Therapeutic Drug Monitoring

2018 ◽  
Vol 15 (1) ◽  
pp. 95-102
Author(s):  
Guiyan Yuan ◽  
Danni Liu ◽  
Fanlong Bu ◽  
Yanyan Wang ◽  
Benjie Wang ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Drug Monitoring ◽  
Severe Infection ◽  
Gradient Elution ◽  
Storage Conditions ◽  
Therapeutic Drug ◽  
Individual Response ◽  
Ionization Source

Background: Therapeutic drug monitoring is recommended for patients taking vancomycin and teicoplanin to ensure pharmaceutical efficacy and prevent toxicity. Only few studies were reported regarding the simultaneous determination of vancomycin and teicoplanin in human plasma. Objective: The study aimed at developing and validating a Liquid Chromatography-Mass Spectrometry (LC-MS) method for simultaneous determination and therapeutic drug monitoring of vancomycin and teicoplanin in patients with severe infection. Method: Plasma was processed by protein precipitation extraction. The analytes were separated on a C18 column by gradient elution with 0.1% formic acid and acetonitrile as mobile phase and measured by electrospray ionization source in positive selective ion monitoring mode at m/z 724.7 (vancomycin), 940.7 (teicoplanin) and 329.0 (bergenin). The plasma samples (104) were obtained from patients who were taking vancomycin or teicoplanin for further analysis. Results: The calibration curves were linear within the range of 0.25–40 µg/mL for vancomycin, and 0.5-40 µg/mL for teicoplanin. Either inter- or intra-day precision was less than 10.01 %. The extraction recoveries ranged from 89.99 to 94.29% for vancomycin and from 39.83 to 40.16 % for teicoplanin. Vancomycin and teicoplanin in plasma were stable at various storage conditions. The measured mean trough concentrations were 12.313 µg/mL for vancomycin and 8.765 µg/mL for teicoplanin. Conclusion: This method was successfully applied to therapeutic drug monitoring of vancomycin and teicoplanin in patients. It is with great clinic value for monitoring and predicting the individual response of patients under treatment.

A simple and automated online SPE-LC-MS/MS method for simultaneous determination of olanzapine, fluoxetine and norfluoxetine in human plasma and its application in therapeutic drug monitoring

RSC Advances ◽  
2015 ◽  
Vol 5 (43) ◽  
pp. 34342-34352 ◽  
Author(s):  
Yiran Fan ◽  
Guanghu Shen ◽  
Ping Li ◽  
Xiaonan Xi ◽  
Haiting Wu ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Drug Monitoring ◽  
Sample Pretreatment ◽  
Therapeutic Drug ◽  
High Quality ◽  
Online Spe

An integration of sample pretreatment automation using online SPE technique could provide an easy to use, efficient, sensitive and high quality methods for TDM.

scholarly journals Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

2011 ◽  
Vol 55 (10) ◽  
pp. 4873-4879 ◽  
Author(s):  
Marie-Clémence Verdier ◽  
Olivier Tribut ◽  
Pierre Tattevin ◽  
Yves Le Tulzo ◽  
Christian Michelet ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Drug Monitoring ◽  
High Performance ◽  
Therapeutic Drug ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics

ABSTRACTA rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 μg/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.

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