scholarly journals Assay Development for Aminoglycosides by HPLC with Direct UV Detection

2016 ◽  
Author(s):  
Bart Blanchaert ◽  
Shengyun Huang ◽  
Katarzyna Wach ◽  
Erwin Adams ◽  
Ann Van Schepdael
Keyword(s):  
Assay Development ◽  
Uv Detection ◽  
Direct Uv Detection

High Pressure Liquid Chromatography with Ultraviolet Absorbance or Fluorescence Detection of Carbaryl in Potato and Corn

1978 ◽  
Vol 61 (4) ◽  
pp. 872-876
Author(s):  
James F Lawrence ◽  
Raymonde Leduc
Keyword(s):  
High Pressure ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
Methylene Chloride ◽  
Sulfonyl Chloride ◽  
Direct Analysis ◽  
Uv Detection ◽  
Uv Absorption ◽  
Fluorescence Excitation ◽  
Direct Uv Detection

Abstract Carbaryl (1 - naphthyl -N - methylcarbamate) was extracted from corn and potato with acetone. The acetone extract was partitioned into methylene chloride-hexane and concentrated for cleanup on a 5 % water-deactivated Florisil column. The fraction containing the carbamate was subjected to high pressure liquid chromatographic (HPLC) analysis on a 25 cm LiChrosorb Si60 (5 >m) column (2.2 mm id) by various means. First, the pesticide was analyzed directly with ultraviolet (UV) absorption detection at 254 nm and a mobile phase of trimethylpentane- isopropanol (96+4). After this, a fluorescent derivative was prepared, using dansyl chloride (5-dimethyIaminonaphthalene- 1-sulfonyl chloride), and analyzed by HPLC, using trimethylpentane-dioxane ( 9 5 + 5 ) as the mobile phase, with both UV absorption (254 nm) and fluorescence (excitation 365 nm, emission >400 nm) detection. The response/ng for the dansyl derivative with fluorescence detection was 1.3 times greater than that for UV detection and 8 times more sensitive than direct UV detection of the carbamate. About 10 ppb carbaryl could be detected after derivatization in the foods studied; for direct analysis, 30—50 ppb could be detected at a 2:1 signal:noise ratio. Recoveries at 0.1 ppm averaged 9 0% by direct analysis compared with 7 8% after derivatization.

Investigation of photochemical reaction products of glucose formed during direct UV detection in CE

2015 ◽  
Vol 37 (7-8) ◽  
pp. 947-953 ◽  
Author(s):  
Thomas Schmid ◽  
Markus Himmelsbach ◽  
Wolfgang W. Buchberger
Keyword(s):  
Photochemical Reaction ◽  
Uv Detection ◽  
Reaction Products ◽  
Direct Uv Detection

Analysis of Organic Acids in Wine by Capillary Electrophoresis with Direct UV Detection

2002 ◽  
Vol 15 (3) ◽  
pp. 319-331 ◽  
Author(s):  
A. Castiñeira ◽  
R.M. Peña ◽  
C. Herrero ◽  
S. Garcı́a-Martı́n
Keyword(s):  
Capillary Electrophoresis ◽  
Organic Acids ◽  
Uv Detection ◽  
Direct Uv Detection
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