scholarly journals Synthesis, HPLC Measurement and Bioavailability of the Phenolic Amide Amkamide

2013 ◽  
Vol 52 (5) ◽  
pp. 436-441
Author(s):  
Jae B. Park
Keyword(s):  
Hplc Measurement

Biodecomposition of Phenanthrene and Pyrene by a Genetically Engineered Escherichia coli

2020 ◽  
Vol 14 (2) ◽  
pp. 121-133 ◽  
Author(s):  
Maryam Ahankoub ◽  
Gashtasb Mardani ◽  
Payam Ghasemi-Dehkordi ◽  
Ameneh Mehri-Ghahfarrokhi ◽  
Abbas Doosti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance ◽  
Human Cancer ◽  
Genetically Engineered ◽  
Hazardous Substances ◽  
E Coli ◽  
Spiked Soil ◽  
Engineered Escherichia Coli ◽  
Genetically Manipulated ◽  
Hplc Measurement

Background: Genetically engineered microorganisms (GEMs) can be used for bioremediation of the biological pollutants into nonhazardous or less-hazardous substances, at lower cost. Polycyclic aromatic hydrocarbons (PAHs) are one of these contaminants that associated with a risk of human cancer development. Genetically engineered E. coli that encoded catechol 2,3- dioxygenase (C230) was created and investigated its ability to biodecomposition of phenanthrene and pyrene in spiked soil using high-performance liquid chromatography (HPLC) measurement. We revised patents documents relating to the use of GEMs for bioremediation. This approach have already been done in others studies although using other genes codifying for same catechol degradation approach. Objective: In this study, we investigated biodecomposition of phenanthrene and pyrene by a genetically engineered Escherichia coli. Methods: Briefly, following the cloning of C230 gene (nahH) into pUC18 vector and transformation into E. coli Top10F, the complementary tests, including catalase, oxidase and PCR were used as on isolated bacteria from spiked soil. Results: The results of HPLC measurement showed that in spiked soil containing engineered E. coli, biodegradation of phenanthrene and pyrene comparing to autoclaved soil that inoculated by wild type of E. coli and normal soil group with natural microbial flora, were statistically significant (p<0.05). Moreover, catalase test was positive while the oxidase tests were negative. Conclusion: These findings indicated that genetically manipulated E. coli can provide an effective clean-up process on PAH compounds and it is useful for bioremediation of environmental pollution with petrochemical products.

Interference of hemoglobin (Hb) Las Palmas with HPLC measurement of HbA1c in 87 patients

2012 ◽  
Vol 50 (2) ◽  
Author(s):  
Mercedes Lorenzo-Medina ◽  
Silvia De La Iglesia ◽  
Paloma Ropero ◽  
Mercedes Navarro-Romero ◽  
Ruth Martín-Alfaro ◽  
...  
Keyword(s):  
Hplc Measurement

HPLC Measurement of the Uronate and Neutral Sugar Contents in Corn Hemicellulose

1993 ◽  
Vol 57 (7) ◽  
pp. 1191-1192 ◽  
Author(s):  
Fumihide Yamaguchi ◽  
Chitoshi Hatanaka
Keyword(s):  
Neutral Sugar ◽  
Hplc Measurement

31-NMR and HPLC measurement of ATP catabolism in the ischemic heart: Is there an NMR-invisible ATP pool?

1992 ◽  
Vol 24 ◽  
pp. S99
Author(s):  
Paul Herijgers ◽  
Kristin Overloop ◽  
Yoshihiro Toshima ◽  
Paul Van Hecke ◽  
Willem Flameng
Keyword(s):  
Ischemic Heart ◽  
Atp Pool ◽  
Hplc Measurement

Improved solid-phase extraction and HPLC measurement of torasemide and its important metabolites

2006 ◽  
Vol 831 (1-2) ◽  
pp. 31-35 ◽  
Author(s):  
Sabine Engelhardt ◽  
Ingolf Meineke ◽  
Jürgen Brockmöller
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Hplc Measurement
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