scholarly journals Method Development and Validation of Olprinone in Human Plasma by HPLC Coupled with ESI-MS-MS: Application to a Pharmacokinetic Study

2013 ◽  
Vol 52 (5) ◽  
pp. 400-405 ◽  
Author(s):  
Hongbo Wang ◽  
Baochang Zhang ◽  
Xilin Sun ◽  
Yantong Sun ◽  
Meiyun Shi
Keyword(s):  
Human Plasma ◽  
Pharmacokinetic Study ◽  
Method Development ◽  
Esi Ms ◽  
Method Development And Validation ◽  
Development And Validation

Introduction of an Innovative approach for Bioanalytical Method Development and Validation of Febuxostat by using LC-ESI-MS/MS in Human Plasma

2021 ◽  
pp. 4060-4066
Author(s):  
Dibya Das ◽  
Dhiman Halder ◽  
Himangshu Sekhar Maji ◽  
Pintu Kumar De ◽  
Sudipta Saha ◽  
...  
Keyword(s):  
Formic Acid ◽  
Human Plasma ◽  
Method Development ◽  
Gradient Elution ◽  
Bioanalytical Method ◽  
Esi Ms ◽  
Validation Parameters ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Plasma Extraction

The aim of the present work is to develop and validate an accurate, sensitive, rapid, precise, and simple bioanalytical method for estimation of Febuxostat in human plasma by using LC-ESI-MS/MS. The method was developed by gradient elution technique with the combination of the mobile phase as 0.1% Formic Acid in Milli-Q water and 0.1% Formic Acid in Acetonitrile at a flow rate of 0.5ml/min. The Analyte and IS (Tolbutamide) were separated by a C18 Phenomenex Kinetex (50x3mm, 5µ) column. The chromatographic run time was 7.0 minutes. The plasma extraction was done by a simple protein precipitation technique (PPT). The LOD and LLOQ were found to be 6.25ng/ml and 125ng/ml, respectively. The extraction recovery of the drug from plasma was more than 90%. The validation parameters were found within the range, as mentioned by USFDA and EMA guidelines.

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