scholarly journals Determination of Atenolol in Human Plasma by HPLC with Fluorescence Detection: Validation and Application in a Pharmacokinetic Study

2012 ◽  
Vol 51 (2) ◽  
pp. 128-132 ◽  
Author(s):  
M. Spanakis ◽  
I. Niopas
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Pharmacokinetic Study

A New HPLC Method with Fluorescence Detection for the Determination of Memantine in Human Plasma

2013 ◽  
Vol 96 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Serife Evrim Kepekci Tekkeli ◽  
Sidika Erturk Toker
Keyword(s):  
Human Plasma ◽  
Fluorescence Detection ◽  
Pharmacokinetic Study ◽  
Mobile Phase ◽  
Orthophosphoric Acid ◽  
Gradient Elution ◽  
Hplc Method ◽  
Fluorometric Detection ◽  
Fluorescent Derivative

Abstract A sensitive and selective HPLC method with fluorometric detection was developed for the determination of memantine in human plasma and applied to a pharmacokinetic study. Memantine was precolumn derivatized with 9-fluorenylmethyl chloroformate, and the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile–10 mM orthophosphoric acid containing 1 mL/L triethylamine with gradient elution. The method was based on the measurement of the derivative using fluorescence detection at 310 nm with excitation at 260 nm. The calibration curve was linear over the range 1.0–50.0 ng/mL. LOD and LOQ were found to be 0.3 and 1.0 ng/mL, respectively. Intraday and interday RSD values were less than 3.39%.

Development and Validation of a New Method for the Determination of Anti-hepatitis C Agent Simeprevir in Human Plasma using HPLC with Fluorescence Detection

2020 ◽  
Vol 16 (4) ◽  
pp. 428-435
Author(s):  
Ahmed F.A. Youssef ◽  
Yousry M. Issa ◽  
Kareem M. Nabil
Keyword(s):  
Hepatitis C ◽  
Human Plasma ◽  
Fluorescence Detection ◽  
Internal Standard ◽  
Protein Precipitation ◽  
Assay Method ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Relative Standard

Background: Simeprevir is one of the recently discovered drugs for treating hepatitis C which is one of the major diseases across the globe. Objective: The present study involves the development of a new and unique High-Performance Liquid Chromatography (HPLC) method using fluorescence detection for the determination of simeprevir (SIM) in human plasma. Methods: Two methods of extractions were tested, protein precipitation using acetonitrile and liquidliquid extraction. A 25 mM dipotassium hydrogen orthophosphate (pH 7.0)/ACN (50/50; v/v), was used as mobile phase and C18 reversed phase column as the stationary phase. The chromatographic conditions were optimized and the concentration of simeprevir was determined by using the fluorescence detector. Cyclobenzaprine was used as an internal standard. Results: Recovery of the assay method based on protein precipitation was up to 100%. Intra-day and inter-day accuracies range from 92.30 to 107.80%, with Relative Standard Deviation (RSD) range 1.65-8.02%. The present method was successfully applied to a pharmacokinetic study where SIM was administered as a single dose of 150 mg SIM/capsule (Olysio®) to healthy individuals. Conclusion: This method exhibits high sensitivity with a low limit of quantification 10 ng mL-1, good selectivity using fluorescence detection, wide linear application range 10-3000 ng mL-1, good recovery and highly precise and validation results. The developed method can be applied in routine analysis for real samples.

Simultaneous determination of ornidazole and its main metabolites in human plasma by LC–MS/MS: application to a pharmacokinetic study

Bioanalysis ◽  
2014 ◽  
Vol 6 (18) ◽  
pp. 2343-2356 ◽  
Author(s):  
Jiangbo Du ◽  
Zhiyu Ma ◽  
Yifan Zhang ◽  
Ting Wang ◽  
Xiaoyan Chen ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Pharmacokinetic Study
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