scholarly journals Nickel Ferrite Nanoparticles as an Adsorbent for Immobilized Metal Affinity Chromatography of Proteins

2020 ◽  
Author(s):  
Kayambu Kannan ◽  
Joyeeta Mukherjee ◽  
Prashant Mishra ◽  
Munishwar N Gupta
Keyword(s):  
Magnetic Material ◽  
Affinity Chromatography ◽  
Nickel Ferrite ◽  
High Capacity ◽  
Ferrite Nanoparticles ◽  
Immobilized Metal Affinity Chromatography ◽  
Simple Method ◽  
Metal Affinity ◽  
Nickel Ferrite Nanoparticles ◽  
Ir Transmission

Abstract A simple method of preparing amorphous nickel ferrite nanoparticles of about 5 nm diameter is described. These particles were characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). The nanoparticles were evaluated for their use as a magnetic material for immobilized metal affinity chromatography (IMAC). The ferrite nanoparticles bound to bovine serum albumin (BSA) and the binding fitted Langmuir isotherm model. A high capacity of 916 mg BSA/g dried nanoparticle was observed. Six proteins (Soybean trypsin inhibitor (STI), lactate dehydrogenase (LDH), papain, catalase, β-galactosidase and casein) were used and all were found to bind at >90% level (except papain which showed 84% binding). All the proteins except LDH and β-galactosidase could be eluted with 1 M imidazole and with % activity recovery of >80%. Papain could be purified from its dried crude latex by 5-fold and purified papain showed a single band on SDS-PAGE. These nanoparticles constitute a high capacity and are magnetic material useful for IMAC and do not require any pre-functionalization.

scholarly journals Synthesis, characterization and heavy metal removal efficiency of nickel ferrite nanoparticles (NFN’s)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Waheed Ali Khoso ◽  
Noor Haleem ◽  
Muhammad Anwar Baig ◽  
Yousuf Jamal
Keyword(s):  
Heavy Metals ◽  
Nickel Ferrite ◽  
Contact Time ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Ferrite Nanoparticles ◽  
Batch Adsorption ◽  
Aqueous Environment ◽  
Human Beings ◽  
Nickel Ferrite Nanoparticles

AbstractThe heavy metals, such as Cr(VI), Pb(II) and Cd(II), in aqueous solutions are toxic even at trace levels and have caused adverse health impacts on human beings. Hence the removal of these heavy metals from the aqueous environment is important to protect biodiversity, hydrosphere ecosystems, and human beings. In this study, magnetic Nickel-Ferrite Nanoparticles (NFNs) were synthesized by co-precipitation method and characterized using X-Ray Diffraction (XRD), Energy Dispersive Spectroscopy (EDS) and Field Emission Scanning Electronic Microscopy (FE-SEM) techniques in order to confirm the crystalline structure, composition and morphology of the NFN’s, these were then used as adsorbent for the removal of Cr(VI), Pb(II) and Cd(II) from wastewater. The adsorption parameters under study were pH, dose and contact time. The values for optimum removal through batch-adsorption were investigated at different parameters (pH 3–7, dose: 10, 20, 30, 40 and 50 mg and contact time: 30, 60, 90, and 120 min). Removal efficiencies of Cr(VI), Pb(II) and Cd(II) were obtained 89%, 79% and 87% respectively under optimal conditions. It was found that the kinetics followed the pseudo second order model for the removal of heavy metals using Nickel ferrite nanoparticles.

Tailoring the photo-Fenton activity of nickel ferrite nanoparticles synthesized by low-temperature coprecipitation technique

Optik ◽  
2017 ◽  
Vol 137 ◽  
pp. 244-253 ◽  
Author(s):  
P. Annie Vinosha ◽  
Belina Xavier ◽  
A. Ashwini ◽  
L. Ansel Mely ◽  
S. Jerome Das
Keyword(s):  
Low Temperature ◽  
Nickel Ferrite ◽  
Ferrite Nanoparticles ◽  
Nickel Ferrite Nanoparticles ◽  
Coprecipitation Technique

FORMATION AND HARD MAGNETIC PROPERTIES OF A HEXAGONAL NETWORK OF NICKEL FERRITE NANOPARTICLES

2006 ◽  
Vol 20 (26) ◽  
pp. 1645-1651
Author(s):  
JIAFU CHEN ◽  
YU YE ◽  
QIANWANG CHEN
Keyword(s):  
Network Structure ◽  
Self Assembly ◽  
Nickel Ferrite ◽  
Ferrite Nanoparticles ◽  
Magnetic Feature ◽  
Bulk Materials ◽  
Nickel Ferrite Nanoparticles ◽  
Carbon Coated ◽  
Hard Magnetic Properties ◽  
Hexagonal Network

A novel hexagonal network structure formed by self-assembly of discrete nickel ferrite nanoparticles on a carbon-coated Cu grid is reported. Each hexagon consists of about 22 discrete nanoparticles with sizes from 120 to 250 nm. The side of the regular hexagon contains 4–6 discrete nanoparticles. The sample displays a large coercivity of 622.6 Oe, exhibiting a hard magnetic feature different from those of the corresponding bulk materials, and is closely related to the hexagonal network structure of nickel ferrite nanoparticles.

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