scholarly journals Validated High-Performance Liquid Chromatographic Technique for Determination of 5-Fluorouracil: Applications to Stability Studies and Simulated Colonic Media

2009 ◽  
Vol 47 (7) ◽  
pp. 558-563 ◽  
Author(s):  
F. K. Alanazi ◽  
A. Eldeen Yassin ◽  
M. El-Badry ◽  
H. A. Mowafy ◽  
I. A. Alsarra
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Technique ◽  
Stability Studies ◽  
Liquid Chromatographic

scholarly journals ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ATOMOXETINE HYDROCHLORIDE USING RAPID HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC TECHNIQUE

2018 ◽  
Vol 11 (11) ◽  
pp. 118
Author(s):  
Zubaidur Rahman ◽  
Vijey Aanandhi M ◽  
Sumithra M
Keyword(s):  
High Performance ◽  
Method Development ◽  
High Sensitivity ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Chromatographic Technique ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc ◽  
Liquid Chromatographic

Objective: A simple, novel, sensitive, rapid high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for quantitative determination of atomoxetine HCl (ATH) in bulk and formulations.Methods: The chromatographic development was carried out on RP-HPLC. The column used as Xterra RP 18 (250 mm × 4.6 mm, 5 μ particle size), with mobile phase consisting of methanol: water 80:20 V/V. The flow rate was 1.0 mL/min and the effluents were monitored at 270 nm.Results: The retention time was found to be 5.350 min. The method was validated as per International Conference on Harmonization Guideline with respect to linearity, accuracy, precision, and robustness. The calibration curve was found to be linear over a range of 2–10 μg/mL with a regression coefficient of 0.9999. The method has proved high sensitivity and specificity.Conclusion: The results of the study showed that the proposed RP-HPLC method was simple, rapid, precise and accurate which is useful for the routine determination of ATH in bulk drug and in its pharmaceutical dosage form.

scholarly journals Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen’s egg white

2011 ◽  
Vol 20 (No. 3) ◽  
pp. 95-97
Author(s):  
A. Prošková ◽  
J. Kučera
Keyword(s):  
Metal Ion ◽  
High Performance ◽  
Copper Ions ◽  
Food Products ◽  
High Performance Liquid Chromatographic ◽  
Egg White ◽  
Chromatographic Technique ◽  
Standard Samples ◽  
Liquid Chromatographic

An immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens – ovomucoid – in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibility was determined. The possible application of this method is discussed. The method is based on the HPLC chromatography on the chelating column saturated with copper ions. The sample is applied to the column at pH 7.5 and eluted isocraticaly with a buffer containing 1.75 mM imidazole in the optimum case.  

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