Determination of Cortisol in Human Plasma by Thin-Layer Chromatography and Fluorescence Derivatization with Isonicotinic Acid Hydrazide

2008 ◽  
Vol 46 (1) ◽  
pp. 1-3 ◽  
Author(s):  
M. Fenske
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Isonicotinic Acid ◽  
Acid Hydrazide ◽  
Isonicotinic Acid Hydrazide ◽  
Fluorescence Derivatization ◽  
Layer Chromatography

THE DETERMINATION OF 5α-ANDROSTANE-3α,17β-DIOL IN HUMAN PLASMA BY RADIOIMMUNOASSAY

1978 ◽  
Vol 88 (4) ◽  
pp. 778-786 ◽  
Author(s):  
P. Laband ◽  
J. A. F. Tresguerres ◽  
B. P. Lisboa ◽  
U. Volkwein ◽  
J. Tamm
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Cross Reactions ◽  
Idiopathic Hirsutism ◽  
Coefficients Of Variation ◽  
The Cross ◽  
Normal Males ◽  
Layer Chromatography

ABSTRACT Antibodies have been raised in rabbits against 3α,17β-dihydroxy-5α-androstane-6-0-carboxymethyloxime coupled with Cohn's fraction IV-4. The antiserum exhibited significant cross reactions with 5β-androstane-3α,17β-diol, 5α-dihydrotestosterone, and testosterone. No cross reactions were observed with 5α-androstane-3β,17β-diol and 5-androstene-3β,17β-diol. The methodological criteria for the measurement of 5α-androstane-3α,17β-diol in human plasma were as follows: The specificity was ensured by separating the cross reacting steroids by thin layer chromatography. The intraassay and interassay coefficients of variation were found to be 6.2 and 10.2 %, respectively. The sensitivity was 30 pg. The recovery of different amounts of 5α-androstane-3α,17β-diol added to human plasma (80, 120, and 200 pg) yielded 91.3, 92.5, and 93.5%, respectively. The following concentrations of 5α-androstane-3α,17β-diol have been determined in human plasma (mean ± sd, ng/dl): Normal males: 18.98 ± 5.9; normal females: 2.65 ± 0.27; females with idiopathic hirsutism: 11.9 ± 6.4; pre-pubertal children: not detectable.

scholarly journals Determination of Cortisol in Human Plasma by Radioimmunoassay

1977 ◽  
Vol 14 (1-6) ◽  
pp. 343-349 ◽  
Author(s):  
G. F. Read ◽  
Diana R. Fahmy ◽  
R. F. Walker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Human Plasma ◽  
Plasma Cortisol ◽  
Specific Radioimmunoassay ◽  
Precision And Accuracy ◽  
Layer Chromatography

A radioimmunoassay for plasma cortisol featuring the gamma-emitting radioligand 125I-iodohistamine, coupled to cortisol-3-(O-carboxymethyl)-oxime, is described. The new procedure retains much of the specificity associated with the use of anti-cortisol-3-BSA sera with tritium-labelled radioligands, and has the further advantages that running costs are lower and there is a greater potential for automation. Cortisol values obtained by this procedure agree well with those obtained by a published specific radioimmunoassay using the tritiated cortisol radioligand. Specificity of the procedure was checked by comparing values obtained with and without thin-layer chromatography purification: correlation was excellent (r = 0·96). Satisfactory levels of sensitivity, precision, and accuracy were obtained.

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