Retention Behavior of  -Carotene on Polar and Nonpolar Stationary Phases in Supercritical Fluid Chromatography

1994 ◽  
Vol 32 (5) ◽  
pp. 172-178 ◽  
Author(s):  
K. Sakaki ◽  
T. Shinbo ◽  
M. Kawamura
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Retention Behavior ◽  
Nonpolar Stationary Phases

scholarly journals Comparison of Retention Behavior between Supercritical Fluid Chromatography and Normal-Phase High-Performance Liquid Chromatography with Various Stationary Phases

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2425 ◽  
Author(s):  
Tsunehisa Hirose ◽  
Daniel Keck ◽  
Yoshihiro Izumi ◽  
Takeshi Bamba
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Retention Factor ◽  
Normal Phase ◽  
Retention Behavior ◽  
Selection Of

The retention behavior of a wide variety of stationary phases was compared in supercritical fluid chromatography (SFC) and normal-phase high-performance liquid chromatography (NP-HPLC). We also attempted to elucidate the retention behavior in SFC by investigating the selectivity of the different stationary phases. SFC separation conditions with polar stationary phases, such as silica gel (SL) and diol (Diol) phases, operate via adsorptions that include hydrophilic and ionic interactions similar to those in NP-HPLC. Moreover, non-polar stationary phases, such as pentabromophenyl (PBr), pyrenylethyl (PYE), and octadecyl (C18), could be used despite the non-polar mobile phase conditions, because the dispersion and π-π interactions were stronger in SFC than in HPLC. These results reflect the selectivity of the stationary phase and its retention factor, thus providing useful information for the selection of appropriate stationary phases for particular analytes.

scholarly journals Development and Validation of Retention Models in Supercritical Fluid Chromatography for Impregnation Process Design

2021 ◽  
Vol 11 (15) ◽  
pp. 7106
Author(s):  
Miaotian Sun ◽  
Zeynep Ülker ◽  
Zhixing Chen ◽  
Sivaraman Deeptanshu ◽  
Monika Johannsen ◽  
...  
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Retention Factor ◽  
Retention Mechanism ◽  
Retention Data ◽  
Retention Models ◽  
Retention Factors ◽  
Polar Solutes ◽  
Porous Matrices

The retention factor is the key quantity for the thermodynamic analysis of the retention mechanism in chromatographic experiments. In this work, we measure retention factors for moderately polar solutes on four silica-based porous matrices as stationary phases by supercritical fluid chromatography. Elution of the solutes is only possible with binary mixtures of supercritical carbon dioxide (sc-CO2) and modifier (methanol) due to the low polarity of pure sc-CO2. The addition of modifiers makes the retention mechanism more complex and masks interactions between solute and stationary phase. In this work, we develop and validate several retention models that allow the obtaining of retention factors in modifier-free sc-CO2. Such models pave the way for quantifying adsorption interactions between polar solutes and non-swellable porous matrices in pure sc-CO2 based on retention data obtained in sc-CO2/modifier mixtures. The obtained information will thereby facilitate the understanding and design of impregnation processes, which are often performed in modifier-free conditions.

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