Correction to--Capillary Column Gas-Liquid Chromatography with Thermal Conductivity Detectors

1963 ◽  
Vol 1 (2) ◽  
pp. 17-17 ◽  
Keyword(s):  
Thermal Conductivity ◽  
Liquid Chromatography ◽  
Capillary Column ◽  
Gas Liquid Chromatography

The Inability of Pyrolysis Gas-Liquid Chromatography to Differentiate Selected Foodborne Bacteria

1982 ◽  
Vol 45 (3) ◽  
pp. 229-233 ◽  
Author(s):  
NORMAN J. STERN
Keyword(s):  
Liquid Chromatography ◽  
Discriminant Analysis ◽  
Capillary Column ◽  
Stepwise Discriminant Analysis ◽  
Gas Liquid Chromatography ◽  
Gram Stain ◽  
Bacterial Strains ◽  
Pyrolysis Gas ◽  
Pyrolysis Products ◽  
Weak Points

Pyrolysis gas-liquid chromatography (PGLC) and stepwise discriminant analysis (SDA) were ineffective when used to differentiate selected genera, species and strains of foodborne microorganisms. Each of 18 individual bacterial strains analyzed was grown, harvested and subjected to PGLC analysis. The resulting pyrolysis products were separated on a high resolution capillary column and the elution patterns (pyrograms) were subjected to stepwise discriminant analysis of 26 (a–z) characteristic peaks. Classification with the combination of PGLC and SDA was 87% accurate for gram-negative strains of bacteria and 94% accurate for gram-positive strains of bacteria. PGLC-stepwise discriminant· analysis correctly discriminated 80% of the bacterial strains according to the known gram-stain reactions. Only 63% were correctly classified to the genus level when all samples were compared. These findings point out the weak points for this method of bacterial analysis.

Identification and metabolic implication of 1-deoxyglucose (1,5-anhydroglucitol) in human plasma.

1982 ◽  
Vol 28 (6) ◽  
pp. 1283-1286 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
T Fujisawa ◽  
A Fujimori ◽  
O Takatani ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Capillary Column ◽  
Physiological Role ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Glass Capillary ◽  
Liquid Chromatograph

Abstract 1-Deoxyglucose (1,5-anhydroglucitol), a metabolite related to diabetes mellitus, was identified in human plasma by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Plasma polyols were accurately determined with a gas-liquid chromatograph equipped with an all-glass capillary column. The plasma content of 1-deoxyglucose in healthy persons varies with age. Although the precise physiological role of 1-deoxyglucose remains obscure, the method described here for determining the minor polyol components of plasma, as well as the findings of 1-deoxyglucose in the plasma of healthy subjects, may be useful for investigating the metabolic roles of 1-deoxyglucose.

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