Helium-induced late preconditioning in the rat heart in vivo †

R. Huhn; A. Heinen; N.C. Weber; S. Hieber; M.W. Hollmann; W. Schlack; B. Preckel

doi:10.1093/bja/aep042

Xenon induces late preconditioning in rat heart in vivo - involvement of COX-II

European Journal of Anaesthesiology ◽

10.1097/00003643-200606001-00002 ◽

2006 ◽

Vol 23 (Supplement 37) ◽

pp. 1

Author(s):

J. Fraessdorf ◽

N. C. Weber ◽

C. Ratajczak ◽

W. Schlack ◽

B. Preckel

Keyword(s):

Rat Heart ◽

Late Preconditioning

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Xenon induces late preconditioning in rat heart in vivo - involvement of COX-II

European Journal of Anaesthesiology ◽

10.1097/00003643-200606001-00231 ◽

2006 ◽

Vol 23 (Supplement 37) ◽

pp. 65

Author(s):

J. Fraessdorf ◽

N. C. Weber ◽

C. Ratajczak ◽

W. Schlack ◽

B. Preckel

Keyword(s):

Rat Heart ◽

Late Preconditioning

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P1188 Inhibition of nitric oxide synthase impairs ischaemia-induced angiogenesis in the rat heart in vivo: a magnetic resonance imaging study

European Heart Journal ◽

10.1016/s0195-668x(03)94480-0 ◽

2003 ◽

Vol 24 (5) ◽

pp. 222

Author(s):

D WALLER

Keyword(s):

Magnetic Resonance Imaging ◽

Nitric Oxide ◽

Magnetic Resonance ◽

Nitric Oxide Synthase ◽

Rat Heart ◽

Imaging Study ◽

Magnetic Resonance Imaging Study ◽

Resonance Imaging ◽

Oxide Synthase

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The in vivo effect of digitoxin on rat heart phosphatides

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)39030-1 ◽

1961 ◽

Vol 2 (2) ◽

pp. 188-190

Author(s):

G.V. Marinetti ◽

K. Temple ◽

Elmer Stotz

Keyword(s):

Rat Heart

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Functional cardiac orexin receptors: role of orexin-B/orexin 2 receptor in myocardial protection

Clinical Science ◽

10.1042/cs20180150 ◽

2018 ◽

Vol 132 (24) ◽

pp. 2547-2564 ◽

Cited By ~ 3

Author(s):

Vanlata H. Patel ◽

Emmanouil Karteris ◽

Jing Chen ◽

Ioannis Kyrou ◽

Harman S. Mattu ◽

...

Keyword(s):

Rat Heart ◽

Troponin I ◽

Clinical Symptoms ◽

Human Subjects ◽

Heart Association ◽

Significant Negative Correlation ◽

In Vivo Model ◽

Rat Cardiomyocytes ◽

Orexin Receptors

Orexins/hypocretins exert cardiovascular effects which are centrally mediated. In the present study, we tested whether orexins and their receptors may also act in an autocrine/paracrine manner in the heart exerting direct effects. Quantitative reverse transcription-PCR (RT-PCR), immunohistochemical and Western blot analyses revealed that the rat heart expresses orexins and orexin receptors (OXR). In isolated rat cardiomyocytes, only orexin-B (OR-B) caused an increase in contractile shortening, independent of diastolic or systolic calcium levels. A specific orexin receptor-2 (OX2R) agonist ([Ala11, d-Leu15]-Orexin B) exerted similar effects as OR-B, whereas a specific orexin receptor-1 (OX1R) antagonist (SB-408124) did not alter the responsiveness of OR-B. Treatment of the same model with OR-B resulted in a dose-dependent increase in myosin light chain and troponin-I (TnI) phosphorylation. Following ischaemia/reperfusion in the isolated Langendorff perfused rat heart model, OR-B, but not OR-A, exerts a cardioprotective effect; mirrored in an in vivo model as well. Unlike OR-A, OR-B was also able to induce extracellular signal-regulated kinase (ERK) 1/2 (ERK1/2) and Akt phosphorylation in rat myocardial tissue and ERK1/2 phosphorylation in human heart samples. These findings were further corroborated in an in vivo rat model. In human subjects with heart failure, there is a significant negative correlation between the expression of OX2R and the severity of the disease clinical symptoms, as assessed by the New York Heart Association (NYHA) functional classification. Collectively, we provide evidence of a distinct orexin system in the heart that exerts a cardioprotective role via an OR-B/OX2R pathway.

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Reduced high-energy phosphate levels in rat hearts. I. Effects of alloxan diabetes

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1976.230.6.1744 ◽

1976 ◽

Vol 230 (6) ◽

pp. 1744-1750 ◽

Cited By ~ 32

Author(s):

TB Allison ◽

SP Bruttig ◽

Crass MF ◽

RS Eliot ◽

JC Shipp

Keyword(s):

Oxidative Metabolism ◽

Oxygen Delivery ◽

Rat Heart ◽

High Energy ◽

Diabetic Rat ◽

High Energy Phosphate ◽

Atp Production ◽

Rat Hearts

Significant alterations in heart carbohydrate and lipid metabolism are present 48 h after intravenous injection of alloxan (60 mg/kg) in rats. It has been suggested that uncoupling of oxidative phosphorylation occurs in the alloxanized rat heart in vivo, whereas normal oxidative metabolism has been demonstrated in alloxan-diabetic rat hearts perfused in vitro under conditions of adequate oxygen delivery. We examined the hypothesis that high-energy phosphate metabolism might be adversely affected in the alloxan-diabetic rat heart in vivo. Phosphocreatine and ATP were reduced by 58 and 45%, respectively (P is less than 0.001). Also, oxygen-dissociation curves were shifted to the left by 4 mmHg, and the rate of oxygen release from blood was reduced by 21% (P is less than 0.01). Insulin administration normalized heart high-energy phosphate compounds. ATP production was accelerated in diabetic hearts perfused in vitro with a well-oxygenated buffer. These studies support the hypothesis that oxidative ATP production in the alloxan-diabetic rat heart is reduced and suggest that decreased oxygen delivery may have a regulatory role in the oxidative metabolism of the diabetic rat heart.

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