scholarly journals RNAblueprint: flexible multiple target nucleic acid sequence design

2017 ◽  
Vol 33 (18) ◽  
pp. 2850-2858 ◽  
Author(s):  
Stefan Hammer ◽  
Birgit Tschiatschek ◽  
Christoph Flamm ◽  
Ivo L Hofacker ◽  
Sven Findeiß
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Sequence ◽  
Multiple Target ◽  
Sequence Design ◽  
Target Nucleic Acid

Nucleic acid sequence design via efficient ensemble defect optimization

2010 ◽  
Vol 32 (3) ◽  
pp. 439-452 ◽  
Author(s):  
Joseph N. Zadeh ◽  
Brian R. Wolfe ◽  
Niles A. Pierce
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Sequence ◽  
Sequence Design

Research on Nucleic Acid Sequence Design Methods for DNA Computing

2009 ◽  
Vol 31 (12) ◽  
pp. 2149-2154
Author(s):  
Kai ZHANG ◽  
Xiu-Tang GENG ◽  
Jian-Hua XIAO ◽  
Dong-Ming ZHAO
Keyword(s):  
Nucleic Acid ◽  
Dna Computing ◽  
Design Methods ◽  
Nucleic Acid Sequence ◽  
Sequence Design

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification

2015 ◽  
Vol 160 (3) ◽  
pp. 719-725 ◽  
Author(s):  
Qiu-Hua Mo ◽  
Hai-Bo Wang ◽  
Hui-Rong Dai ◽  
Ji-Can Lin ◽  
Hua Tan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Simultaneous Detection ◽  
Nucleic Acid Sequence

Complete nisin A gene cluster from Lactococcus lactis M78 (HM219853) — obtaining the nucleic acid sequence and comparing it to other published nisin sequences

2011 ◽  
Vol 33 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Aljoša Trmčić ◽  
John Samelis ◽  
Christophe Monnet ◽  
Irena Rogelj ◽  
Bojana Bogovič Matijašić
Keyword(s):  
Nucleic Acid ◽  
Gene Cluster ◽  
Lactococcus Lactis ◽  
Nucleic Acid Sequence

Viral nucleic acid sequence transfer between fungi and plants

1997 ◽  
Vol 13 (7) ◽  
pp. 260-261 ◽  
Author(s):  
Joachim R. Marienfeld ◽  
Michael Unseld ◽  
Petra Brandt ◽  
Axel Brennicke
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Sequence ◽  
Viral Nucleic Acid
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