scholarly journals Electronically subtracting expression patterns from a mixed cell population

2007 ◽  
Vol 23 (24) ◽  
pp. 3328-3334 ◽  
Author(s):  
Mark M. Gosink ◽  
Howard T. Petrie ◽  
Nicholas F. Tsinoremas
Keyword(s):  
Cell Population ◽  
Expression Patterns ◽  
Mixed Cell ◽  
Mixed Cell Population

scholarly journals Characterization of nonspecific esterase activity in macrophages and intestinal epithelium of the rat.

1990 ◽  
Vol 38 (1) ◽  
pp. 1-6 ◽  
Author(s):  
A W Stadnyk ◽  
A D Befus ◽  
J Gauldie
Keyword(s):  
Cell Population ◽  
Peritoneal Macrophages ◽  
Nonspecific Esterase ◽  
Intestinal Epithelial ◽  
Mixed Cell ◽  
Tissue Sections ◽  
Prominent Component ◽  
Mixed Cell Population ◽  
Morphological Criteria ◽  
Different Populations

We determined the histochemical characteristics of nonspecific esterase in different populations of rat macrophages. The cells included alveolar and peritoneal macrophages recovered by lavage and a mixed cell population obtained by collagenase digestion of the small intestine. The histochemically localized enzyme activity of alveolar and peritoneal macrophages was cytoplasmic, diffuse, and inhibited by sodium fluoride. Both populations were effectively stained using alpha-naphthyl acetate and alpha-naphthyl butyrate as the esterase substrate. When the intestinal cells were examined for activity, a greater percentage of cells showed positive nonspecific esterase than would be predicted by differential counts for macrophages on the basis of morphological criteria. We confirmed, using cell smears and tissue sections, that rat intestinal epithelial cells, a prominent component of the isolated cell population, possessed esterases that react similarly to macrophage esterases with histochemical procedures.

In vitro isolation of Plasmodium knowlesi merozoites using polycarbonate sieves

Parasitology ◽  
1975 ◽  
Vol 71 (3) ◽  
pp. 475-481 ◽  
Author(s):  
E. D. Dennis ◽  
G. H. Mitchell ◽  
G. A. Butcher ◽  
S. Cohen
Keyword(s):  
Pore Size ◽  
Cell Population ◽  
Malaria Parasite ◽  
Plasmodium Knowlesi ◽  
High Yield ◽  
Culture Chamber ◽  
Mixed Cell ◽  
Mixed Cell Population ◽  
Antigenic Properties

A culture chamber fitted with a polycarbonate sieve has been used to isolate Plasmodium knowlesi merozoites as they are released from schizonts. A 3 μm pore-size sieve allows passage of normal erythrocytes and red cells containing rings and trophozoites and can be used to concentrate schizonts from a mixed cell population. A 2 μm pore-size sieve retains normal and parasitized cells and provides uncontaminated merozoites in high yield (5 × 1010 merozoites per ml schizonts). Merozoite viability diminishes rapidly during 30 min after isolation. These preparations should prove valuable for studies of the biochemical, physiological and antigenic properties of this transient extracellular phase of the malaria parasite.

scholarly journals Significance of the inital cytomorphological and immunocytochemical findings and the correlation with the international prognostic index for the survival in patients with non-Hodgkin’s lymphoma

2006 ◽  
Vol 63 (1) ◽  
pp. 31-36
Author(s):  
Biljana Mihaljevic ◽  
Ruzica Nedeljkov-Jancic ◽  
Vesna Cemerikic-Martinovic
Keyword(s):  
Cell Population ◽  
Fine Needle Aspiration ◽  
International Prognostic Index ◽  
Prognostic Index ◽  
Large Cell ◽  
Needle Aspiration ◽  
Small Cells ◽  
Mixed Cell ◽  
Fine Needle ◽  
Mixed Cell Population

Background/Aim. Fine-needle aspiration biopsy is a quick, economical, and safe initial method in managing a patient with suspected lymphoma. According to a few reports on this preoblem, the aim of this study was to compare histological findings to cytomorphological ones in needle aspirates. We also compared these findings to the overal survival (OS) time. Methods. We analyzed the fine-needle aspiration biopsies of peripheral lymph nodes, and the International Prognostic Index (IPI) in 81 patients with non-Hodgkin?s lymphoma (NHL). We put these findings into correlation with OS time. Results. According to the International Working Formulation (IWF) criteria, the dominant cell population was as follows: 18 patients had the small cell population, 21 patients had small cleaved cells, 18 patients had the mixed cell population, 21 patients had large cell population, 2 patients had Burkitt lymphoma type, and 1 patient had the dominant lymphoblasts. On presentation, 32 patients had a low IPI index, 32 patients had a low intermediate, and 17 patients had a high intermediate IPI. We confirmed the statistical significance (Kaplan-Mayer) of cytomorphology (p = 0.013) and IPI index (p = 0.016) for survival time. During a 48-month follow-up, OS was 37.2 months for the patients with the dominant small cells, and 32 months for the patients with small cleaved cells (PH equivalent to indolent NHL). For the patients with the dominant mixed cell population, large cell population and Burkitt limphoma cell, OS were 17, 14.4, and 9.3 months, respectively (PH equivalent to aggressive NHL). Patients with low IPI had the highest OS, 36 months for the low intermediate and only 11.6 months for the high intermediate IPI index. Conclusion. We concluded that an initial cytological and clinical profile of patients with NHL, might give a quick and relevant information for planning an adequate therapy.

Primary Dural Melanoma

Neurosurgery ◽  
1981 ◽  
Vol 9 (6) ◽  
pp. 710-717 ◽  
Author(s):  
Raj K. Naravan ◽  
Michael J. Rosner ◽  
John T. Povlishock ◽  
Alexander Girevendulis ◽  
Donald P. Becker
Keyword(s):  
Electron Microscopy ◽  
Cell Population ◽  
Cerebellopontine Angle ◽  
Tumor Recurrence ◽  
Concerted Effort ◽  
Mixed Cell ◽  
Mixed Cell Population ◽  
Homogenous Cell Population ◽  
Ultrastructural Findings ◽  
Electron Lucent

Abstract This study reviews the literature pertaining to primary meningeal melanoma and reports the clinical and ultrastructural findings in a case where the tumor appeared to be of pachymeningeal (dural) origin. This is clearly a departure from all previously described cases, in which a leptomeningeal (pial-arachnoidal) origin was either defined or assumed. Clinically. this case was remarkable in its rarity. its presentation as a cerebellopontine angle syndrome, and its occurrence in a Negro. a race in which melanomas are uncommon. Ultrastructurally. the tumor did demonstrate the presence of basement membrane abnormalities and numerous endothelial fenestrations. However, it was found to be made up of a homogenous cell population, consisting only of electron-lucent, melanin-laden cells. The mixed cell population noted previously in a primary leptomeningeal melanoma was not found in this tumor. In view of the fact that this patient continues to do well 1/12; years after operation, with no evidence of tumor recurrence, it is suggested that a homogenous cell population noted on electron microscopy could indicate a better prognosis. In addition, it may also indicate a pachymeningeal rather than a leptomeningeal origin for the tumor. A plea is made for greater specificity in terminology when describing primary meningeal melanomas and for a concerted effort to distinguish between those of dural and those of leptomeningeal origin.

scholarly journals Quantitation of mast cell heparin by flow cytofluorometry.

1976 ◽  
Vol 24 (12) ◽  
pp. 1231-1238 ◽  
Author(s):  
L Enerbäck ◽  
G Berlin ◽  
I Svensson ◽  
I Rundquist
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Mixed Cell ◽  
Flow Cytofluorometry ◽  
Frequency Distributions ◽  
Cell Counts ◽  
Mixed Cell Population ◽  
Number Of Cells ◽  
Excellent Reproducibility

Mast cells can be automatically identified in a mixed cell population by flow cytofluorometry after Berberine sulphate staining. Volume specific counts of the total number of cells and number of mast cells, as well as frequency distributions of fluorescence intensities of mast cells, based on a large number of cells, can be rapidly obtained. Results obtained by microscope fluorometry of cells identified by phase contrast microscopy showviously published results it may be inferred that the fluorescence intensity of individual mast cells is proportional to mast cell heparin content. The automated cell counts correlated very well with manual hemocytometer counts. Both cell counts and the determination of mean mast cell fluorescence showed excellent reproducibility.

The response of a mixed cell population in mouse testes to x-ray and HZE irradiation

1983 ◽  
Vol 41 ◽  
pp. 674-675
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
J. Stevenson ◽  
S. Black ◽  
...  
Keyword(s):  
Radiation Effects ◽  
Dose Range ◽  
Life Cycles ◽  
X Rays ◽  
Mixed Cell ◽  
Particle Radiation ◽  
Thin Sections ◽  
Straight Line ◽  
Mixed Cell Population ◽  
Standard Manner

The testes sperraatogonial population differentiates from a stem cell (As) which, via a series of mitotic divisions, gives rise to spermatocytes. The spermatocytes undergo reductional meiotic divisions and mature into sperm. Short cellular life cycles make the testes an excellent tissue for quantitative studies of radiation effects.Brown B6D2F1 mice, aged 14 weeks, were exposed to Helium particle radiation (227 MeV) in the Berkeley 184 inch synchocyclotron and to x rays at 225 KvP and 15 mA. Doses ranged from 1 to 100 rads. Tissues were fixed in Triple Fix and embedded. Two-micron sections were stained with Toluidine Blue, and ultra-thin sections were stained in the standard manner for electron microscopy. All studies were done on the tissues 72 hrs after irradiation.Our previous work, on cell survival after graded doses of radiation, has shown a steeper slope (more sensitive response) in the low dose range below 20 rads. By convention, the resulting points were plotted by linear regression resulting in a "best fit" straight line.

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