scholarly journals Emerging gene knockout technology in zebrafish: zinc-finger nucleases

2008 ◽  
Vol 7 (6) ◽  
pp. 460-464 ◽  
Author(s):  
S. L. Amacher
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Knockout Technology ◽  
Gene Knockout Technology

Production of myostatin gene knockout Wuzhishan miniature pig fibroblasts with zinc-finger nucleases

2013 ◽  
Vol 35 (6) ◽  
pp. 778-785
Author(s):  
Sui-Zhong CAO ◽  
Cheng-He YUE ◽  
Xi-Rui LI ◽  
Chong FENG ◽  
Chuan LONG ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Miniature Pig ◽  
Myostatin Gene

scholarly journals Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhaolin Sun ◽  
Ming Wang ◽  
Shiwen Han ◽  
Shuangyu Ma ◽  
Zhiyuan Zou ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Beta Lactoglobulin

scholarly journals Histone Deacetylase Inhibition Rescues Gene Knockout Levels Achieved with Integrase-Defective Lentiviral Vectors Encoding Zinc-Finger Nucleases

2013 ◽  
Vol 24 (6) ◽  
pp. 399-411 ◽  
Author(s):  
Laetitia P.L. Pelascini ◽  
Ignazio Maggio ◽  
Jin Liu ◽  
Maarten Holkers ◽  
Toni Cathomen ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Zinc Finger ◽  
Gene Knockout ◽  
Lentiviral Vectors ◽  
Zinc Finger Nucleases ◽  
Histone Deacetylase Inhibition

scholarly journals Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28911 ◽  
Author(s):  
Anna Osiak ◽  
Frank Radecke ◽  
Eva Guhl ◽  
Sarah Radecke ◽  
Nadine Dannemann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Zinc Finger ◽  
Knockout Mouse ◽  
Gene Knockout ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Zinc Finger Nucleases ◽  
Gene Knockout Mouse

333 ESTABLISHMENT OF NOVEL METHOD FOR REPEATED CONSTRUCTION OF ENGINEERED ZINC FINGER NUCLEASE

2013 ◽  
Vol 25 (1) ◽  
pp. 314
Author(s):  
W. Fujii ◽  
K. Kano ◽  
K. Sugiura ◽  
K. Naito
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Cell Stage ◽  
Endonuclease Domain ◽  
Pcr Products ◽  
Novel Method ◽  
Recognition Helix ◽  
Primer Sets

Zinc finger nucleases (ZFN), which are artificial restriction enzymes consisting of an engineered zinc-finger domain (ZF) and an endonuclease domain, can be used for the induction of site-directed mutation and the efficient generation of gene knockout animals. However, the repeated construction of various ZFN sequences is both expensive and time consuming. In this study, we attempted to establish a novel method for inexpensive and rapid ZFN construction. First, we constructed ZFN against mouse Rosa26 and original mouse Gli3 gene loci using short PCR primer sets (>30 bp), which contained 21 bp of the ZF recognition helix for a specific DNA triplet. We prepared 18 sets of such primers and PCR was performed using one of these primer sets and the partial ZF sequence as a template, which was obtained from the first to second DNA recognition helix of mouse Zif268. The PCR products were joined by overlap-PCR and nested PCR, and then inserted into a vector coding the endonuclease domain of FokI nuclease. By these steps, we successfully synthesised intended ZFN vectors containing 4 to 6 fingers. Next, we evaluated the functions of constructed ZFN. The mRNA of constructed ZFN were transcribed in vitro and injected into the cytoplasm of C57BL/6N zygotes. After 24 h of culture, 2-cell stage embryos were subjected to genomic PCR of the target locus, and the PCR products were directly sequenced. When ZFN mRNA for mouse Rosa26 was injected, 3- to 146-bp deletions were detected in 92.8% of injected embryos. This result was almost the same as previously reported for ZFN, indicating that our novel construction method can synthesise functional ZFN, which work as a site-directed nuclease, and that efficiency was comparable with those constructed by conventional PCR methods using long oligonucleotide sets (60 bp).

Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Pei-Qi Liu ◽  
Edmond M. Chan ◽  
Gregory J. Cost ◽  
Lin Zhang ◽  
Jianbin Wang ◽  
...  
Keyword(s):  
Cell Line ◽  
Zinc Finger ◽  
Mammalian Cell ◽  
Gene Knockout ◽  
Zinc Finger Nucleases ◽  
Mammalian Cell Line

Gene knockout and knockin by zinc-finger nucleases: current status and perspectives

2012 ◽  
Vol 70 (16) ◽  
pp. 2969-2983 ◽  
Author(s):  
J. Hauschild-Quintern ◽  
B. Petersen ◽  
G. J. Cost ◽  
H. Niemann
Keyword(s):  
Zinc Finger ◽  
Gene Knockout ◽  
Current Status ◽  
Zinc Finger Nucleases
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