Subtyping Lymphocytes in Peripheral Blood by Direct Immunoalkaline Phosphatase Labeling and Light Scatter/Absorption Flow Cytometric Analysis

1992 ◽  
Vol 97 (3) ◽  
pp. 331-337
Author(s):  
Young Ran Kim ◽  
Lynn Paseltiner ◽  
Gail Kling ◽  
Chien Kuo Yeh
Keyword(s):  
Peripheral Blood ◽  
Flow Cytometric Analysis ◽  
Light Scatter ◽  
Flow Cytometric

scholarly journals Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Serkan Yazıcı ◽  
Emel Bülbül Başkan ◽  
Ferah Budak ◽  
Barbaros Oral ◽  
Şaduman Balaban Adim ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mycosis Fungoides ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Flow Cytometric Analysis ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Flow Cytometric ◽  
Hla Dr ◽  
The Mean

We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society for Cutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3+, CD4+, CD8+, TCRαβ+, TCRγδ+, CD7+, CD4+CD7+, CD4+CD7−, and CD71+), B cells (HLA-DR+, CD19+, and HLA-DR+CD19+), NKT cells (CD3+CD16+CD56+), and NK cells (CD3−CD16+CD56+). The mean value of all cell surface antigens was not statistically significant between parapsoriasis and MF groups. Along with an increase in cases of MF stage statistically significant difference was found between the mean values of cell surface antigens. Flow cytometric analysis of peripheral blood cell surface antigens in patients with mycosis fungoides may contribute to predicting disease stage and progression.

Flow cytometric enumeration of absolute lymphocyte number in peripheral blood using two parameters of light scatter

Cytometry ◽  
1985 ◽  
Vol 6 (2) ◽  
pp. 172-174 ◽  
Author(s):  
Y. C. Smart ◽  
J. Cox ◽  
B. Murphy ◽  
A. Enno ◽  
R. C. Burton
Keyword(s):  
Peripheral Blood ◽  
Light Scatter ◽  
Flow Cytometric ◽  
Two Parameters ◽  
Lymphocyte Number

Reduced numbers of peripheral blood CD27+ IgD– memory B cells in patients with aggressive periodontitis

2015 ◽  
Vol 39 (3) ◽  
Author(s):  
Holger Jentsch ◽  
Regina Purschwitz ◽  
Catalina Suzana Stingu ◽  
Jana Schmidt ◽  
Ulrich Sack
Keyword(s):  
B Cells ◽  
Peripheral Blood ◽  
Substantial Reduction ◽  
Flow Cytometric Analysis ◽  
Aggressive Periodontitis ◽  
Memory B Cells ◽  
Multifactorial Disease ◽  
Periodontal Condition ◽  
Flow Cytometric ◽  
And Function

AbstractAggressive periodontitis (AgP) is a multifactorial disease with unknown association to the development and function of peripheral lymphocytes. The aim of this study was to elucidate a connection between the periodontal condition in 10 patients with AgP and their potential state of immunodeficiency. Based on full periodontal examination and radiographs, 10 females (ages 29.8±8.62 years) with established diagnosis of aggressive periodontitis were included in this study. Flow cytometric analysis revealed substantial reduction of switched memory B cells (IgM

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