scholarly journals Identification of reference genes for qRT-PCR in human lung squamous-cell carcinoma by RNA-Seq

2014 ◽  
Vol 46 (4) ◽  
pp. 330-337 ◽  
Author(s):  
Cheng Zhan ◽  
Yongxing Zhang ◽  
Jun Ma ◽  
Lin Wang ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Reference Genes ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Rna Seq ◽  
Qrt Pcr

scholarly journals Distinctive roles of syntaxin binding protein 4 and its action target, TP63, in lung squamous cell carcinoma: a theranostic study for the precision medicine

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Erkhem-Ochir Bilguun ◽  
Kyoichi Kaira ◽  
Reika Kawabata-Iwakawa ◽  
Susumu Rokudai ◽  
Kimihiro Shimizu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Multivariate Analysis ◽  
Cell Carcinoma ◽  
Precision Medicine ◽  
Squamous Cell ◽  
Binding Protein ◽  
Lung Squamous Cell Carcinoma ◽  
Rna Seq ◽  
Cox Regression Analysis ◽  
The Impact

Abstract Background Lung squamous cell carcinoma (LSCC) remains a challenging disease to treat, and further improvements in prognosis are dependent upon the identification of LSCC-specific therapeutic biomarkers and/or targets. We previously found that Syntaxin Binding Protein 4 (STXBP4) plays a crucial role in lesion growth and, therefore, clinical outcomes in LSCC patients through regulation of tumor protein p63 (TP63) ubiquitination. Methods To clarify the impact of STXBP4 and TP63 for LSCC therapeutics, we assessed relevance of these proteins to outcome of 144 LSCC patients and examined whether its action pathway is distinct from those of currently used drugs in in vitro experiments including RNA-seq analysis through comparison with the other putative exploratory targets and/or markers. Results Kaplan–Meier analysis revealed that, along with vascular endothelial growth factor receptor 2 (VEGFR2), STXBP4 expression signified a worse prognosis in LSCC patients, both in terms of overall survival (OS, p = 0.002) and disease-free survival (DFS, p = 0.041). These prognostic impacts of STXBP4 were confirmed in univariate Cox regression analysis, but not in the multivariate analysis. Whereas, TP63 (ΔNp63) closely related to OS (p = 0.013), and shown to be an independent prognostic factor for poor OS in the multivariate analysis (p = 0.0324). The action pathway of STXBP4 on suppression of TP63 (ΔNp63) was unique: Ingenuity pathway analysis using the knowledge database and our RNA-seq analysis in human LSCC cell lines indicated that 35 pathways were activated or inactivated in association with STXBP4, but the action pathway of STXBP4 was distinct from those of other current drug targets: STXBP4, TP63 and KDR (VEGFR2 gene) formed a cluster independent from other target genes of tumor protein p53 (TP53), tubulin beta 3 (TUBB3), stathmin 1 (STMN1) and cluster of differentiation 274 (CD274: programmed cell death 1 ligand 1, PD-L1). STXBP4 itself appeared not to be a potent predictive marker of individual drug response, but we found that TP63, main action target of STXBP4, might be involved in drug resistance mechanisms of LSCC. Conclusion STXBP4 and the action target, TP63, could afford a key to the development of precision medicine for LSCC patients.

scholarly journals RNA-Seq profiling of circular RNA in human lung adenocarcinoma and squamous cell carcinoma

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Chengdi Wang ◽  
Shuangyan Tan ◽  
Wen-Rong Liu ◽  
Qian Lei ◽  
Wenliang Qiao ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Lung Adenocarcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Human Lung ◽  
Circular Rna ◽  
Rna Seq ◽  
Human Lung Adenocarcinoma

Purification of a Tumoral Marker Recognized by Monoclonal Antibody Po66 and Associated with Human Lung Squamous Cell Carcinoma

1996 ◽  
Vol 11 (3) ◽  
pp. 148-152 ◽  
Author(s):  
F. Brichory ◽  
B. Collet ◽  
C. Pineau ◽  
B. Desrues ◽  
L. Toujas ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Squamous Cell ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Squamous Carcinoma ◽  
Prolonged Retention ◽  
Squamous Carcinoma Cell

Monoclonal antibody (MAb) Po66, a murine IgG1, was raised by immunization against human lung squamous cell carcinoma. When injected intravenously, Po66 showed prolonged retention in the tumor. It recognized an intracellular antigen. The human lung squamous carcinoma cell line SK-MES-1 expresses the antigen recognized by MAb Po66 and was used as a source of biological material for its purification. The SK-MES-1 cell line was labeled in culture with [35S]methionine and its lysate was immunoprecipitated with Po66 immobilized on Protein G-Sepharose. The precipitate contained three proteins (47, 50 and 69 kDa) absent in the controls. The 69 kDa polypeptide was further purified by anion exchange and immunoaffinity chromatographies. To date, no other tumor marker expressed in non-small cell lung cancer with these characteristics has been described and as such this marker is interesting for future use in immunotherapy and in diagnosis.

scholarly journals Terminal N-Acetylgalactosamine-Specific Leguminous Lectin from Wisteria japonica as a Probe for Human Lung Squamous Cell Carcinoma

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83886 ◽  
Author(s):  
Keisuke Soga ◽  
Futaba Teruya ◽  
Hiroaki Tateno ◽  
Jun Hirabayashi ◽  
Kazuo Yamamoto
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Leguminous Lectin

Cytogenetic comparison of two poorly differentiated human lung squamous cell carcinoma lines

1992 ◽  
Vol 59 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Elizabeth Law ◽  
Una Gilvarry ◽  
Vincent Lynch ◽  
Bernard Gregory ◽  
Geraldine Grant ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Poorly Differentiated

scholarly journals Identification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma

Cell Research ◽  
2016 ◽  
Vol 26 (10) ◽  
pp. 1149-1164 ◽  
Author(s):  
Fei Li ◽  
Zhaoyuan Fang ◽  
Jian Zhang ◽  
Chen Li ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Human Lung ◽  
Lung Squamous Cell Carcinoma ◽  
Oncogenic Driver
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