Why characterise flexible structures? A radarsat project perspective

Progress in high-resolution CRYO-SEM imaging of viral particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166555 ◽

1996 ◽

Vol 54 ◽

pp. 818-819

Author(s):

Ya Chen ◽

Geoffrey Letchworth ◽

John White

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

High Resolution ◽

Structural Information ◽

Signal To Noise Ratio ◽

Flexible Structures ◽

Simian Virus ◽

Topographic Features ◽

Viral Particles ◽

Scanning Electron

Low-temperature high-resolution scanning electron microscopy (cryo-HRSEM) has been successfully utilized to image biological macromolecular complexes at nanometer resolution. Recently, imaging of individual viral particles such as reovirus using cryo-HRSEM or simian virus (SIV) using HRSEM, HV-STEM and AFM have been reported. Although conventional electron microscopy (e.g., negative staining, replica, embedding and section), or cryo-TEM technique are widely used in studying of the architectures of viral particles, scanning electron microscopy presents two major advantages. First, secondary electron signal of SEM represents mostly surface topographic features. The topographic details of a biological assembly can be viewed directly and will not be obscured by signals from the opposite surface or from internal structures. Second, SEM may produce high contrast and signal-to-noise ratio images. As a result of this important feature, it is capable of visualizing not only individual virus particles, but also asymmetric or flexible structures. The 2-3 nm resolution obtained using high resolution cryo-SEM made it possible to provide useful surface structural information of macromolecule complexes within cells and tissues. In this study, cryo-HRSEM is utilized to visualize the distribution of glycoproteins of a herpesvirus.

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