The Karyotype of Microsternarchus aff. bilineatus: A First Case of Y Chromosome Degeneration in Gymnotiformes

Zebrafish ◽  
2017 ◽  
Vol 14 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Jéssica Almeida Batista ◽  
Adauto Lima Cardoso ◽  
Susana Suely Rodrigues Milhomem-Paixão ◽  
Jonathan Stuart Ready ◽  
Julio Cesar Pieczarka ◽  
...  
Author(s):  
N. Kumari ◽  
S. Prasad ◽  
A. K. Pandey ◽  
S. Dash ◽  
R. Sinha

Sex Sorted Semen gives the liberty of producing offspring of the desired sex - in farming animals by using it in conjunction with other assisted reproductive technologies such as Artificial Insemination and In-Vitro Fertilization after selecting the healthy sperm and separating into X-Female and Y-male Chromosome bearing populations based on their DNA content. It is an important biotechnological tool to increase the milk production and the profitability of Dairy Industry. Current study deals with the Principle, methods, main method, advantages, disadvantages and the current status of Sex sorted semen in India and Jharkhand. The main emphasis of this study is to draw the attention of Scientific fraternity towards the effect of Sex Sorted Semen on Population dynamics. The Sex Sorted semen increases the deviation of ratio between Male and Female Population from ideal 1:1, thereby decreasing the effective population size Ne and thus slowly reducing the viability and survivability of the population or breed concerned. Further the already depleting Y chromosomes will be reaped off all its genes in long run at a faster rate due to antagonistic selection pressure arising out of Artificial selection via Sex Sorted Semen acting against all the gene of Y chromosome of the breed or population concerned which might disturb many vital genes and the associated functions. The degeneration and extinction of scientists have been predicted long ago. The effect of Sex Sorted semen on Y- chromosome degeneration is yet to be pointed out, calculated and subsequently verified in any of the literatures. SSS is indeed a boon for India as well as Jharkhand. It might be too early to predict about the negative impact of SSS on population dynamics and Y-Chromosome degeneration. Further research work must be done to assess the extent and authencity of above mentioned impact( Predicted  theoretically) by calculation as well as practical field based Experimentation.


Author(s):  
Paris Veltsos ◽  
Guillaume Cossard ◽  
Emmanuel Beaudoing ◽  
Genséric Beydon ◽  
Camille Roux ◽  
...  

Many dioecious plants have sex chromosomes that are cytologically heteromorphic, but about half of species lack cytological differences between males and females and are thus homomorphic. Very little is known about the size and content of the non-recombining sex-determining region (SDR) in these species. Here, we assess the size and content of the SDR of the diploid dioecious herb Mercurialis annua, which has homomorphic sex chromosomes and shows signatures of mild Y-chromosome degeneration. We used RNAseq to identify new Y-linked markers for M. annua. Twelve of 24 transcripts with male-specific and male-biased expression could only be PCR-amplified from males and are thus Y-linked. We found a further six Y-linked sequences that were present in males but not females using genome capture data from multiple populations. We used the Y-linked sequences to identify and sequence 17 sex-linked bacterial artificial chromosomes (BACs), which form 11 groups of non-overlapping sequence, covering a total sequence length of about 1.5 Mb. Content analysis of this region suggests it is enriched for repeats, has a low gene density and contains few candidate sex-determining genes. The BACs map to a subset of the sex-linked region of the genetic map, which is estimated to be at least 14.5 Mb. This is substantially larger than estimates for other dioecious plants with homomorphic sex chromosomes, especially given the small genome size of M. annua. Our data provide a rare, high-resolution view of the homomorphic Y chromosome of a dioecious plant.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Ana R. Flores ◽  
João Lobo ◽  
Francisco Nunes ◽  
Alexandra Rêma ◽  
Paula Lopes ◽  
...  

Abstract Background Gonadoblastoma (GB) is a rare mixed germ cell-sex cord-stromal tumour, first described in humans, commonly found in dysgenetic gonads of intersex patients that have a Y chromosome. However, this entity in not recognized in the WHO classification of tumours of genital system of domestic animals. Herein, we describe a case of ovarian gonadoblastoma with proliferation of dysgerminoma and sex cord-stromal tumour components, in a phenotypically and cytogenetically normal bitch. Case presentation A 17-year-old cross-breed bitch had a firm, grey-white multinodular mass in the left ovary. The tumour was submitted to histopathological examination and Y chromosome detected through karyotype analysis and PCR studies. Microscopically, the ovary was almost replaced by an irregular neoplasm composed of three distinct, intermixed elements: dysgerminoma, mixed germ cell-sex cord-stromal tumour resembling human GB and a proliferative sex cord-stromal tumour component. The germ cells of gonadoblastoma and dysgerminoma components were immunoreactive for c-KIT. Sex cord-stromal cells of gonadoblastoma were immunoreactive for α-inhibin. The sex cord-stromal tumour was immunoreactive for AE1/AE3, occasionally for α-inhibin and negative for epithelial membrane antigen (EMA). The karyotype was 78, XX and PCR analysis confirmed the absence of the Y chromosome. Conclusion Based on these findings, a diagnosis of gonadoblastoma with proliferation of dysgerminoma and sex cord-stromal tumour was made. This is the first case of ovarian gonadoblastoma in a female dog.


2019 ◽  
Vol 5 (1) ◽  
pp. 1-18
Author(s):  
Sam Taylor-Alexander ◽  
Sharyn Davies

We bring together conceptual readings of time and temporality to discuss evolutionary theories of Y chromosome degeneration as they are spoken about in scientific and popular forums. In doing so, we suggest that debates over Y chromosome degeneration involve a form of abduction – tacking back and forth between different pasts, presents, futures – that frames templates for producing and securing sexed and gendered presents. Here we are using ‘sexed’ as a way of talking about physical bodies and ‘gendered’ as social ways of constructing those sexed bodies. We suggest that arguments over Y chromosome degeneration are as important for current debates surrounding sex, gender, science, molecular biology and a “crisis of masculinity” as they are for (ascertaining) the future of human evolution.


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