Chemically Induced Intestinal Damage Models in Zebrafish Larvae

Zebrafish ◽  
2013 ◽  
Vol 10 (2) ◽  
pp. 184-193 ◽  
Author(s):  
Stefan H. Oehlers ◽  
Maria Vega Flores ◽  
Christopher J. Hall ◽  
Kazuhide S. Okuda ◽  
John Oliver Sison ◽  
...  
Keyword(s):  
Intestinal Damage ◽  
Damage Models ◽  
Zebrafish Larvae ◽  
Chemically Induced

scholarly journals Exploration of Anti-inflammatory Mechanism of Forsythiaside A and Forsythiaside B in CuSO4-Induced Inflammation in Zebrafish by Metabolomic and Proteomic Analyses

2019 ◽  
Author(s):  
Lihong Gong ◽  
Linyuan Yu ◽  
Xiaohong Gong ◽  
Cheng Wang ◽  
Naihua Hu ◽  
...  
Keyword(s):  
Sugar Metabolism ◽  
Amino Sugar ◽  
Zebrafish Model ◽  
Zebrafish Larvae ◽  
Chemically Induced ◽  
Inflammatory Mechanism ◽  
Forsythia Suspensa ◽  
Proteomic Analyses ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract Background: Inflammation is a general pathological phenomenon that operates during severe disturbance of homeostasis. Forsythiaside A (FA) and Forsythiaside B (FB) are isolated from air-dried fruits of Forsythia suspensa, which show a significant effect against inflammation. However, the anti-inflammatory effect and therapeutic mechanism have not yet been clarified in zebrafish. Methods: In this study, the anti-inflammatory effects of FA and FB were investigated in CuSO4-induced zebrafish model. Intracellular ROS and NO generation in zebrafish were performed using fluorescent probe dyes. Metabolomics and proteomics using liquid chromatography mass spectrometry were carried out to identify the expression of metabolites and proteins associated with chemically induced inflammation in zebrafish larvae. Quantitative PCR was performed to detect the progressive changes in the genes.Results: FA and FB inhibited neutrophils migration to the damaged neuromasts and remarkably reduced CuSO4-induced ROS and NO generation in zebrafish. Metabolomics analyses indicated that the Nicotinate and nicotinamide metabolism, Amino sugar and nucleotide sugar metabolism, Pyrimidine metabolism and Purine metabolism were mainly involved. The proteomic analyses identified 146 differentially expressed proteins, among which, the expression of collagen (col2a1b, col9a2, col9a1b), nme3, wdr3, mrps7, srpx, gch2, ptpn11a, rcvrn2, nit2, scaf4a and zgc:162509 were all reversed both in FA and FB groups. FA and FB could reverse the abnormal expression of these metabolites and proteins to alleviate chemical damage to the neuromasts in the lateral line of zebrafish.Conclusions: FA and FB possess remarkable anti-inflammatory effect and could be used to protect chemically induced neuromasts damage in zebrafish larvae.

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

10.3791/63251 ◽  
2021 ◽  
Author(s):  
Jie Ding ◽  
Rui Yan ◽  
Luna Wang ◽  
Qianlei Yang ◽  
Xiaoyun Zhang ◽  
...  
Keyword(s):  
Bone Loss ◽  
Alizarin Red ◽  
Zebrafish Larvae ◽  
Chemically Induced

scholarly journals Protective Role of Galanin during Chemically Induced Inflammation in Zebrafish Larvae

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 99
Author(s):  
Natalia Nowik ◽  
Anna Przyborowska ◽  
Waldemar Sienkiewicz ◽  
Piotr Podlasz
Keyword(s):  
Pathological Condition ◽  
Protective Role ◽  
Neuroendocrine System ◽  
Biological Processes ◽  
Zebrafish Larvae ◽  
Chemically Induced ◽  
Enhanced Expression ◽  
The Central Nervous System ◽  
Endocrine Axis

During a pathological condition, many different systems are involved in the response of an affected organism. Galanin is considered to be a neuropeptide that plays an important role in the central nervous system; however, it is involved in many other biological processes, including the immune response. During our studies, we showed that galanin became upregulated in zebrafish larvae when exposed to copper sulfate. Moreover, the presence of normal levels of galanin, administration of a galanin analog NAX 5055 or galanin overexpression led to lowered lateral line damage and enhanced expression of inflammatory markers compared to the knockout larvae. The results showed that the neuroendocrine system acts multifunctionally and should be considered as a part of the complex neuro–immune–endocrine axis.

Biological and Surface Properties of C-Type Virus Particles Produced by Novikoff Ascites Hepatoma Cells

1977 ◽  
Vol 35 ◽  
pp. 388-389
Author(s):  
D.C. Hixson ◽  
J.C. Chan ◽  
J.M. Bowen ◽  
E.F. Walborg
Keyword(s):  
Surface Properties ◽  
Ricinus Communis ◽  
Rat Kidney ◽  
Leukemia Virus ◽  
Viral Envelope ◽  
Virus Particles ◽  
Chemically Induced ◽  
Sarcoma Virus ◽  
Hepatocarcinoma Cells ◽  
Type C

Several years ago Karasaki (1) reported the production of type C virus particles by Novikoff ascites hepatocarcinoma cells. More recently, Weinstein (2) has reported the presence of type C virus particles in cell cultures derived from transplantable and primary hepatocellular carcinomas. To date, the biological function of these virus and their significance in chemically induced hepatocarcinogenesis are unknown. The present studies were initiated to determine a possible role for type C virus particles in chemically induced hepatocarcinogenesis. This communication describes results of studies on the biological and surface properties of type C virus associated with Novikoff hepatocarcinoma cells.Ecotropic and xenotropic murine leukemia virus (MuLV) activity in ascitic fluid of Novikoff tumor-bearing rats was assayed in murine sarcoma virus transformed S+L- mouse cells and S+L- mink cells, respectively. The presence of sarcoma virus activity was assayed in non-virus-producing normal rat kidney (NRK) cells. Ferritin conjugates of concanavalin A (Fer-Con wheat germ agglutinin (Fer-WGA), and Ricinus communis agglutinins I and II (Fer-RCAI and Fer-RCAII) were used to probe the structure and topography of saccharide determinants present on the viral envelope.

Mechanism underlying protection by constitutively formed nitric oxide of indodmethacin-induced small intestinal damage in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A703-A703
Author(s):  
A TANAKA ◽  
H MIZOGUCHI ◽  
T MIYAZAWA ◽  
T KUNIKATA ◽  
K TAKEUCHI
Keyword(s):  
Nitric Oxide ◽  
Intestinal Damage ◽  
Small Intestinal

Caspase-8 acts as a tumor suppressor in chemically induced hepatocarcinogenesis in mice

2014 ◽  
Vol 52 (01) ◽  
Author(s):  
HM Zimmermann ◽  
N Moro ◽  
R Sonntag ◽  
JM Bangen ◽  
YA Nevzorova ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Caspase 8 ◽  
Chemically Induced

Hepatic B cell leukemia-3 attenuates chemically-induced hepatocarcinogenesis in mice

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
N Gehrke ◽  
MA Wörns ◽  
Y Alt ◽  
A Waisman ◽  
N Hoevelmeyer ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Leukemia ◽  
Chemically Induced ◽  
B Cell Leukemia
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