scholarly journals A Bilayer Engineered Skin Substitute for Wound Repair in an Irradiation-Impeded Healing Model on Rat

2015 ◽  
Vol 4 (5) ◽  
pp. 312-320 ◽  
Author(s):  
A.B. Mohd Hilmi ◽  
Asma Hassan ◽  
Ahmad Sukari Halim
Keyword(s):  
Wound Repair ◽  
Skin Substitute ◽  
Healing Model

Functional Bilayered Skin Substitute Constructed by Tissue-Engineered Extracellular Matrix and Microsphere-Incorporated Gelatin Hydrogel for Wound Repair

2009 ◽  
Vol 15 (9) ◽  
pp. 2617-2624 ◽  
Author(s):  
Sha Huang ◽  
Yongjie Zhang ◽  
Liang Tang ◽  
Zhihong Deng ◽  
Wei Lu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Repair ◽  
Skin Substitute ◽  
Gelatin Hydrogel

Tissue-Engineered Skin Substitutes Using Collagen Scaffold with Amniotic Membrane Component

2005 ◽  
Vol 277-279 ◽  
pp. 12-18 ◽  
Author(s):  
Eun Kyung Yang ◽  
Jung Keug Park ◽  
Jae Il Ahn ◽  
Hee Gu Lee ◽  
Seong Jun Seo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Wound Repair ◽  
Collagen Scaffold ◽  
Animal Origin ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Membrane Component ◽  
Early Degradation

Human skin substitutes are needed for implantation and wound repair based on the new concept of tissue engineering in combination with biomaterials and cell biological technology. However, failure sometimes occurs when the wound healing is delayed in vivo due to acute inflammation resulting from the early degradation of the transplanted biomaterials. Accordingly, the current study modified conventional biomaterials to overcome early degradation and strong inflammation. In a conventional skin substitute, the animal origin collagenous materials have a slight antigenicity as xenogenic materials, however, the modified method was able to obtain a low antigenicity and anti-inflammation effect using atelo-collagen and an amniotic component. The tyrosine content in the developed atelo-collagen, representing the antigenicity, was reduced from 0.590% to 0.046% based on an HPLC analysis. In addition, to reduce the inflammation and foreign material reaction, an amniotic component was applied to the atelo-collagen materials. While, to enhance the wound healing, the modified skin substitute was developed as a composite matrix of an atelo-collagen scaffold with an amniotic membrane component. A quantitative analysis of hEGF in the amniotic membrane was also performed using different processing methods. Finally, a tissueengineered skin substitute was constructed by cultivating skin cells in the collagen scaffold attached to an amniotic membrane.

scholarly journals Pediatric Thermal Burns and Treatment: A Review of Progress and Future Prospects

2017 ◽  
Author(s):  
Elton Mathias ◽  
Madhu Srinivas Murthy
Keyword(s):  
Wound Repair ◽  
Wound Care ◽  
Point Of View ◽  
Burn Injuries ◽  
Skin Substitute ◽  
Future Prospects ◽  
Skin Substitutes ◽  
Burn Wound ◽  
Burn Wounds ◽  
Basic Care

Burns is a pervasive and oppressive basic care issue. In children, burn injuries are a major reason for bleakness and mortality. The quirks in the physiology of liquid and electrolyte taking care of, the vital necessity and the distinctions in the different body extends in children direct that the pediatric wounds administration ought to be brought with an alternate point of view than for adults. Notwithstanding, for the intensivist, challenges regularly exist that muddle quiet help and adjustment. Moreover, burn injuries are mind-boggling and can show exceptional challenges that require deep-rooted recovery. Investigation in burn wound care has yielded progressions that will keep on improving practical recuperation. What's more, pain management all through this period is essential. Managing these wounds requires escalated therapeutic treatment for multi-organ dysfunction, and forceful surgical treatment to forestall sepsis and other inconveniences. The biological therapeutic bilayered skin substitutes with a long shelf life that recapitulates the normal barrier function of the intact human skin and stimulate wound repair and skin regeneration. A definitive objective is to accomplish a perfect skin substitute that gives a successful and without scar wound recuperating. This review article features the headway in pediatric burn wounds with an emphasis on the pathophysiology and treatment of burn wounds.

scholarly journals A Comparative Study of Engineered Dermal Templates for Skin Wound Repair in a Mouse Model

2020 ◽  
Vol 21 (12) ◽  
pp. 4508 ◽  
Author(s):  
Ilia Banakh ◽  
Perdita Cheshire ◽  
Mostafizur Rahman ◽  
Irena Carmichael ◽  
Premlatha Jagadeesan ◽  
...  
Keyword(s):  
Mouse Model ◽  
Wound Repair ◽  
Human Fibroblasts ◽  
Skin Wound ◽  
Tissue Growth ◽  
Skin Substitute ◽  
Protein Levels ◽  
Collagen Iii ◽  
Synthetic Grafts ◽  
Dermal Template

Engineered dermal templates have revolutionised the repair and reconstruction of skin defects. Their interaction with the wound microenvironment and linked molecular mediators of wound repair is still not clear. This study investigated the wound bed and acellular “off the shelf” dermal template interaction in a mouse model. Full-thickness wounds in nude mice were grafted with allogenic skin, and either collagen-based or fully synthetic dermal templates. Changes in the wound bed showed significantly higher vascularisation and fibroblast infiltration in synthetic grafts when compared to collagen-based grafts (P ≤ 0.05). Greater tissue growth was associated with higher prostaglandin-endoperoxide synthase 2 (Ptgs2) RNA and cyclooxygenase-2 (COX-2) protein levels in fully synthetic grafts. Collagen-based grafts had higher levels of collagen III and matrix metallopeptidase 2. To compare the capacity to form a double layer skin substitute, both templates were seeded with human fibroblasts and keratinocytes (so-called human skin equivalent or HSE). Mice were grafted with HSEs to test permanent wound closure with no further treatment required. We found the synthetic dermal template to have a significantly greater capacity to support human epidermal cells. In conclusion, the synthetic template showed advantages over the collagen-based template in a short-term mouse model of wound repair.

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

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