Evaluation of a Direct Immunofluorescent Assay and/or Conjunctival Cytology for Detection ofCanine Distemper VirusAntigen

2018 ◽  
Vol 31 (3) ◽  
pp. 272-275 ◽  
Author(s):  
Labrini V. Athanasiou ◽  
Maria C. Kantere ◽  
Constantinos S. Kyriakis ◽  
Dimitra Pardali ◽  
Katerina Adamama Moraitou ◽  
...  
Keyword(s):  
Immunofluorescent Assay

scholarly journals Using diffusion tensor imaging and immunofluorescent assay to evaluate the pathology of multiple sclerosis

2011 ◽  
Vol 33 (3) ◽  
pp. 557-564 ◽  
Author(s):  
Lauren V. Zollinger ◽  
Tae Ho Kim ◽  
Kenneth Hill ◽  
Eun K. Jeong ◽  
John W. Rose
Keyword(s):  
Multiple Sclerosis ◽  
Diffusion Tensor Imaging ◽  
Diffusion Tensor ◽  
Immunofluorescent Assay

scholarly journals Quality Control Assessment of Canadian Laboratories Testing for Lyme Disease

1991 ◽  
Vol 2 (1) ◽  
pp. 41-45
Author(s):  
Harvey Artsob ◽  
Maxwell Garvie
Keyword(s):  
Public Health ◽  
Quality Control ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Western Blot ◽  
Etiological Agent ◽  
Immunofluorescent Assay ◽  
Satisfactory Performance ◽  
Endemic Areas ◽  
Negative Controls

In June 1990 a quality control assessment was undertaken of Canadian public health laboratories testing for antibodies toBorrelia burgdorferi, the etiological agent of Lyme disease. Twenty sera were distributed to nine laboratories, including 12 obtained from patients in Lyme endemic areas and presumed to be serological positives, and eight prescreened negative controls. Seventeen serological reports were submitted, comprising nine enzyme-linked immunosorbent assays (elisa), six immunofluorescent assays and two Western blot assessments. Antibodies were detected in 11 of the 12 sera which had been presumed to be positive. Assuming 11 positive sera had been submitted, the test sensitivities varied from 88.9 to 100% byelisa, and 54.5 to 90.1% by immunofluorescent assay. Specificities were 100% for all but oneelisaand one immunofluorescent assay assessment. The results indicate a satisfactory performance byelisabut a need for upgrading or replacement of some immunofluorescent assay tests.

scholarly journals Unusual Maculopapular Rash on the Scalp of a Patient with Mediterranean Spotted Fever

2018 ◽  
Vol 28 (3) ◽  
pp. 291-293
Author(s):  
Ivan Baltadzhiev ◽  
Nedialka Popivanova
Keyword(s):  
Breast Cancer ◽  
Clinical Features ◽  
Rare Case ◽  
Clinical Characteristics ◽  
Clinical Presentation ◽  
Spotted Fever ◽  
Good Condition ◽  
Maculopapular Rash ◽  
Mediterranean Spotted Fever ◽  
Immunofluorescent Assay

Objective: To report a rare case of maculopapular rash on the scalp in a patient with Mediterranean spotted fever (MSF). Clinical Presentation and Intervention: A 58-year-old woman with breast cancer and chemotherapy-induced alopecia contracted MSF. Her clinical features were typical, except for a maculopapular rash covering the scalp. The diagnosis of MSF was confirmed by immunofluorescent assay. The disease had a favorable course and the patient was discharged in good condition. Conclusion: The rash on the scalp described in this report enriches our knowledge on the clinical characteristics of MSF.

scholarly journals Serological Survey of Ehrlichia canis and Anaplasma phagocytophilum in Dogs from Central Italy: An Update (2013–2017)

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 3 ◽  
Author(s):  
Valentina Virginia Ebani
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Central Italy ◽  
Ehrlichia Canis ◽  
Serological Survey ◽  
Immunofluorescent Assay ◽  
Indirect Immunofluorescent Assay ◽  
Canine Population ◽  
Indirect Immunofluorescent

Ehrlichia canis and Anaplasma phagocytophilum are tick-borne bacteria of veterinary concern. Indirect immunofluorescent assay was carried out to detect antibodies against E. canis and A. phagocytophilum in 1026 owned dogs living in Central Italy during the period 2013–2017. One hundred and eighty-six (18.12%) dogs were positive for at least one pathogen and 14 (1.36%) for both agents. More in detail, 166 (16.18%) samples were positive for E. canis and 34 (3.31%) for A. phagocytophilum. No statistically significant differences in the seroprevalence values related to gender were detected, whereas the highest rate to E. canis occurred in animals aged more than 10 years. Mean seroprevalence values for both E. canis and A. phagocytophilum detected in 2014 and 2015 were statistically higher with respect to other years. Even though dogs’ owners are informed about the risk of pet infections by tick-borne pathogens and prophylaxis against ticks is often executed, E. canis and A. phagocytophilum are still present and infect the canine population in Central Italy.

scholarly journals Potential Impact of Different Cytomegalovirus (CMV) IgM Assays on an Algorithm Requiring IgM Reactivity as a Criterion for Measuring CMV IgG Avidity

2014 ◽  
Vol 21 (6) ◽  
pp. 813-816 ◽  
Author(s):  
Harry E. Prince ◽  
Mary Lapé-Nixon ◽  
Andrew Brenner ◽  
Nancy Pitstick ◽  
Marc Roger Couturier
Keyword(s):  
Cmv Infection ◽  
Serum Samples ◽  
Immunofluorescent Assay ◽  
Negative Results ◽  
Igg Avidity ◽  
Positive Serum ◽  
Chemiluminescent Immunoassay ◽  
Potential Impact ◽  
The Impact ◽  
Positive Results

ABSTRACTThe measurement of cytomegalovirus (CMV) IgG avidity is a powerful tool for identifying individuals with recent CMV infection. Because such patients are expected to be positive for CMV IgM, several investigators have suggested that CMV IgG-positive sera first be screened for CMV IgM and then only the IgM-reactive sera be tested for avidity. We investigated the impact of different CMV IgM assays on such a reflexing algorithm using a panel of 369 consecutive IgG-positive serum samples submitted for avidity testing. A bead-based immunofluorescent assay (BIFA) identified 105 IgM-positive serum samples, whereas an IgM-capture enzyme immunoassay (EIA) identified 48 IgM-positive serum samples; this marked difference led us to evaluate additional CMV IgM assays. An enzyme-linked immunofluorescent assay (ELFA) and a chemiluminescent immunoassay (CIA) were used to test all sera with discordant BIFA/EIA results, all sera with concordant positive results, and selected sera with concordant negative results. The findings indicated that the ELFA would identify 74 CMV IgM-positive samples and the CIA would identify 64. Of the 23 low-avidity serum samples, 2 were IgM negative by BIFA, 3 by ELFA and CIA, and 4 by EIA; of the 23 intermediate-avidity serum samples, 6 were IgM negative by BIFA, 10 by ELFA, and 15 by EIA and CIA. In both these avidity groups, BIFA IgM-negative sera were also negative by the other 3 assays. These findings demonstrate that an algorithm requiring CMV IgM reactivity as a criterion for CMV IgG avidity testing does not identify all low-avidity sera and thus misses some cases of acute CMV infection.

scholarly journals Evaluation of Immunomagnetic Separation for Recovery of Cryptosporidium parvum and Giardia duodenalis from High-Iron Matrices

2000 ◽  
Vol 66 (8) ◽  
pp. 3628-3631 ◽  
Author(s):  
Gary P. Yakub ◽  
Kathleen L. Stadterman-Knauer
Keyword(s):  
Cryptosporidium Parvum ◽  
Giardia Duodenalis ◽  
Immunomagnetic Separation ◽  
Assay Method ◽  
High Iron ◽  
Immunofluorescent Assay ◽  
Dissolved Iron ◽  
Iron Concentrations

ABSTRACT In this study we examined the recovery of Cryptosporidium parvum and Giardia duodenalis from matrices containing various concentrations of dissolved iron. The organisms were recovered by using the immunomagnetic separation-immunofluorescent assay method, and the levels of recovery were compared to the dissolved iron concentrations. The levels of recovery of C. parvumdecreased sharply at dissolved iron concentrations greater than 4 mg/liter, while the levels of recovery of G. duodenalisdecreased sharply at concentrations greater than 40 mg/liter.

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