Measles Virus Genotype D Wild Strains Suppress Interferon-Stimulated Gene Expression More Potently than Laboratory Strains in SiHa Cells

2016 ◽  
Vol 29 (5) ◽  
pp. 296-306
Author(s):  
Masaru Jinushi ◽  
Soh Yamamoto ◽  
Noriko Ogasawara ◽  
Hideki Nagano ◽  
Shin Hashimoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Measles Virus ◽  
Virus Genotype ◽  
Siha Cells ◽  
Wild Strains

scholarly journals Measles virus genotype D4 strains with non-standard length M-F non-coding region circulated during the major outbreaks of 2011-2012 in Spain

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199975 ◽  
Author(s):  
Horacio Gil ◽  
Aurora Fernández-García ◽  
María Mar Mosquera ◽  
Judith M. Hübschen ◽  
Ana M. Castellanos ◽  
...  
Keyword(s):  
Standard Length ◽  
Measles Virus ◽  
Coding Region ◽  
Virus Genotype

scholarly journals Complete Genome Sequences of Measles Virus Genotype D8 Isolates from South Korea in 2016

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
You-Jin Kim ◽  
Hae Ji Kang ◽  
Su-Jin Kim ◽  
Hye Min Lee ◽  
Sung Soon Kim
Keyword(s):  
Genetic Diversity ◽  
South Korea ◽  
Complete Genome ◽  
Measles Virus ◽  
Virus Transmission ◽  
Wild Type ◽  
Genome Sequences ◽  
Virus Genotype

The complete genome sequences of three wild-type measles viruses (genotype D8) isolated from patients in South Korea were determined. These are the first reported complete genome sequences of measles viruses obtained from South Korea, and the availability of these sequences will improve our understanding of measles virus transmission and genetic diversity.

Genetic characterization of measles virus genotype D6 subacute sclerosing panencephalitis case, Alberta, Canada

2018 ◽  
Vol 24 (6) ◽  
pp. 720-729 ◽  
Author(s):  
K. Pabbaraju ◽  
K. Fonseca ◽  
S. Wong ◽  
M. W. Koch ◽  
J. T. Joseph ◽  
...  
Keyword(s):  
Measles Virus ◽  
Genetic Characterization ◽  
Subacute Sclerosing Panencephalitis ◽  
Virus Genotype

scholarly journals Novel Measles Virus Genotype, East Timor and Australia

2002 ◽  
Vol 8 (7) ◽  
pp. 735-737 ◽  
Author(s):  
Doris Chibo ◽  
Michaela Riddell ◽  
Michael Catton ◽  
Christopher Birch
Keyword(s):  
Measles Virus ◽  
East Timor ◽  
Virus Genotype

scholarly journals Identification and Characterization of a Regulatory Domain on the Carboxyl Terminus of the Measles Virus Nucleocapsid Protein

2002 ◽  
Vol 76 (17) ◽  
pp. 8737-8746 ◽  
Author(s):  
Xinsheng Zhang ◽  
Candace Glendening ◽  
Hawley Linke ◽  
Christopher L. Parks ◽  
Charles Brooks ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Binding Affinity ◽  
Reporter Gene ◽  
Nucleocapsid Protein ◽  
Measles Virus ◽  
Reporter Gene Expression ◽  
Regulatory Domain ◽  
N Protein ◽  
Basal Activity

ABSTRACT The paramyxovirus template for transcription and genome replication consists of the RNA genome encapsidated by the nucleocapsid protein (N protein). The activity of the complex, consisting of viral polymerase plus template, can be measured with minireplicons in which the genomic coding sequence is replaced by chloramphenical acetyltransferase (CAT) antisense RNA. Using this approach, we showed that the C-terminal 24 amino acids of the measles virus N protein are dispensable for transcription and replication, based upon the truncation of N proteins used to support minireplicon reporter gene expression. Truncation at the C-terminal or penultimate amino acid 524 resulted in no change in CAT expression, whereas larger truncations spanning residues 523 to 502 were accompanied by an approximately twofold increase in basal activity. Reporter gene expression was enhanced by supplementation with the major inducible 70-kDa heat shock protein (Hsp72) for minireplicons with the N protein or the N protein truncated at position 525 or 524 but not in systems with a truncation at position 523 or 522. Naturally occurring sequence variants of the N protein with variations at positions 522 and 523 were also shown to lack Hsp72 responsiveness independent of changes in basal activity. Since these residues lie within a linear sequence predicting a direct Hsp72 interaction, N protein-Hsp72 binding reactions were analyzed by using surface plasmon resonance technology. Truncation of the C-terminal portion of the N protein by protease digestion resulted in a reduced binding affinity between Hsp72 and the N protein. Furthermore, with synthetic peptides, we established a correlation between the functional responsiveness and the binding affinity for Hsp72 of C-terminal N protein sequences. Collectively, these results show that the C-terminal 24 amino acids of the N protein represent a regulatory domain containing a functional motif that mediates a direct interaction with Hsp72.

scholarly journals Measles virus induces cell-type specific changes in gene expression

Virology ◽  
2008 ◽  
Vol 375 (2) ◽  
pp. 321-330 ◽  
Author(s):  
Hiroki Sato ◽  
Reiko Honma ◽  
Misako Yoneda ◽  
Ryuichi Miura ◽  
Kyoko Tsukiyama-Kohara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Measles Virus ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Linkage mapping of yeast cross protection connects gene expression variation to a higher-order organismal trait

2017 ◽  
Author(s):  
Tara N. Stuecker ◽  
Amanda N. Scholes ◽  
Jeffrey A. Lewis
Keyword(s):  
Gene Expression ◽  
Natural Variation ◽  
Higher Order ◽  
Cross Protection ◽  
Ethanol Stress ◽  
Gene Expression Variation ◽  
Gene Expression Response ◽  
Expression Variation ◽  
Wild Strains ◽  
Stress Defense

AbstractGene expression variation is extensive in nature, and is hypothesized to play a major role in shaping phenotypic diversity. However, connecting differences in gene expression across individuals to higher-order organismal traits is not trivial. In many cases, gene expression variation may be evolutionarily neutral, and in other cases expression variation may only affect phenotype under specific conditions. To understand connections between gene expression variation and stress defense phenotypes, we have been leveraging extensive natural variation in the gene expression response to acute ethanol in laboratory and wild Saccharomyces cerevisiae strains. Previous work found that the genetic architecture underlying these expression differences included dozens of “hotspot” loci that affected many transcripts in trans. In the present study, we provide new evidence that one of these expression QTL hotspot loci is responsible for natural variation in one particular stress defense phenotype—ethanol-induced cross protection against severe doses of H2O2. The causative polymorphism is in the heme-activated transcription factor Hap1p, which we show directly impacts cross protection, but not the basal H2O2 resistance of unstressed cells. This provides further support that distinct cellular mechanisms underlie basal and acquired stress resistance. We also show that the Hap1p-dependent cross protection relies on novel regulation of cytosolic catalase T (Ctt1p) during ethanol stress in wild strains. Because ethanol accumulation precedes aerobic respiration and accompanying reactive oxygen species formation, wild strains with the ability to anticipate impending oxidative stress would likely be at an advantage. This study highlights how strategically chosen traits that better correlate with gene expression changes can improve our power to identify novel connections between gene expression variation and higher-order organismal phenotypes.

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