Immune Responses to Mucosal Vaccination by the Recombinant S1 and N Proteins of Infectious Bronchitis Virus

2012 ◽  
Vol 25 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Rosie Meir ◽  
Simi Krispel ◽  
Lubov Simanov ◽  
Dalia Eliahu ◽  
Ora Maharat ◽  
...  
Keyword(s):  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Mucosal Vaccination ◽  
N Proteins

scholarly journals Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge

Vaccine ◽  
2018 ◽  
Vol 36 (19) ◽  
pp. 2630-2636 ◽  
Author(s):  
Priscila Diniz Lopes ◽  
Cintia Hiromi Okino ◽  
Filipe Santos Fernando ◽  
Caren Pavani ◽  
Viviane Mariguela Casagrande ◽  
...  
Keyword(s):  
Immune Responses ◽  
Virus Vaccine ◽  
Infectious Bronchitis Virus ◽  
Chitosan Nanoparticles ◽  
Effective Protection ◽  
Infectious Bronchitis ◽  
Mucosal Immune Responses

scholarly journals Greater Protection against Virulent Infectious Bronchitis Virus Challenge Conferred by Recombinant Baculovirus Co-expressing S1 and N Proteins

2018 ◽  
Author(s):  
Yuan Yuan ◽  
Zhi-Peng Zhang ◽  
Yi-Ning He ◽  
Wen-Sheng Fan ◽  
Zhi-Hua Dong ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Infectious Bronchitis Virus ◽  
Subunit Vaccine ◽  
Recombinant Baculovirus ◽  
Recombinant Baculoviruses ◽  
Economic Losses ◽  
Subunit Vaccines ◽  
Infectious Bronchitis ◽  
Antibody Levels ◽  
N Proteins

Avian infectious bronchitis virus (IBV) is the causative agent of infectious bronchitis, which causes considerable economic losses to the poultry industry worldwide. It is imperative to develop safe and efficient candidate vaccines to control IBV infection. In the current study, recombinant baculoviruses co-expressing S1 and N proteins, mono-expressing S1 or N proteins alone of IBV were constructed and prepared into subunit vaccines rHBM-S1-N, rHBM-S1 and rHBM-N. The levels of immune protection of these subunit vaccines were evaluated by inoculating specific pathogen-free (SPF) chickens at 14 days of age, boosting with the same dose 14 days later, and following challenge with a virulent GX-YL5 strain of IBV 14 days post-booster (dpb). The commercial vaccine strain H120 was used as a control. The IBV-specific antibody levels as well as the percentages of CD4+ and CD8+ T lymphocytes were detected within 28 days post-vaccination (dpv). The morbidity, mortality, and re-isolation of virus from the tracheas and kidneys of challenged birds were evaluated at 5 days post-challenge (dpc). The results showed that the IBV-specific antibody levels and the percentages of CD4+ and CD8+ T lymphocyte in rHBM-S1-N group were higher than those of rHBM-S1 and rHBM-N groups, especially the cellular immunity response. At 5 dpc, the mortality, morbidity and virus re-isolation rate of rHBM-S1-N were slightly higher than those of H120 group, but were lower than those of rHBM-S1 group and rHBM-N group. The present study demonstrated that the protection of recombinant baculovirus co-expressing S1 and N proteins was better than that of recombinant baculoviruses mono-expressing S1 or N protein alone. Thus, the recombinant baculovirus co-expressing S1 and N proteins could serve as a potential IBV vaccine and this demonstrates that the bivalent subunit vaccine including the S1 and N proteins might be a strategy for the development of an IBV subunit vaccine.

scholarly journals Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Mohammed Al-Rasheed ◽  
Christopher Ball ◽  
Kannan Ganapathy
Keyword(s):  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Laying Hens ◽  
Early Time ◽  
Harderian Gland ◽  
Vaccine Virus ◽  
Egg Laying ◽  
Immune Gene ◽  
Mrna Levels ◽  
Infectious Bronchitis

AbstractChicken immune responses to infectious bronchitis virus (IBV) vaccination can depend on route of administration, vaccine strain and bird age. Typically for layer chickens, IBV vaccinations are administered by spray in the hatchery at day-old and boosted at intervals with live vaccines via drinking water (DW). Knowledge of live attenuated IBV vaccine virus kinetics and the immune response in egg-laying hens is exceptionally limited. Here, we demonstrated dissemination of vaccine viruses and differences in hen innate, mucosal, cellular and humoral immune responses following vaccination with Massachusetts or 793B strains, administered by DW or oculonasal (ON) routes. Detection of IBV in the Mass-vaccinated groups was greater during early time-points, however, 793B was detected more frequently at later timepoints. Viral RNA loads in the Harderian gland and turbinate tissues were significantly higher for ON-Mass compared to all other vaccinated groups. Lachrymal fluid IgY levels were significantly greater than the control at 14 days post-vaccination (dpv) for both vaccine serotypes, and IgA mRNA levels were significantly greater in ON-vaccinated groups compared to DW-vaccinated groups, demonstrating robust mucosal immune responses. Cell mediated immune gene transcripts (CD8-α and CD8-β) were up-regulated in turbinate and trachea tissues. For both vaccines, dissemination and vaccine virus clearance was slower when given by DW compared to the ON route. For ON administration, both vaccines induced comparable levels of mucosal immunity. The Mass vaccine induced cellular immunity to similar levels regardless of vaccination method. When given either by ON or DW, 793B vaccination induced significantly higher levels of humoral immunity.

scholarly journals Proteomic analysis of chicken bone marrow-derived dendritic cells in response to an inactivated IBV + NDV poultry vaccine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Robin H. G. A. van den Biggelaar ◽  
Larissa van der Maas ◽  
Hugo D. Meiring ◽  
Jeroen L. A. Pennings ◽  
Willem van Eden ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Proteomic Analysis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Infectious Bronchitis ◽  
Chicken Bone ◽  
Poultry Vaccine

AbstractInactivated poultry vaccines are subject to routine potency testing for batch release, requiring large numbers of animals. The replacement of in vivo tests for cell-based alternatives can be facilitated by the identification of biomarkers for vaccine-induced immune responses. In this study, chicken bone marrow-derived dendritic cells were stimulated with an inactivated vaccine for infectious bronchitis virus and Newcastle disease virus, as well as inactivated infectious bronchitis virus only, and lipopolysaccharides as positive control, or left unstimulated for comparison with the stimulated samples. Next, the cells were lysed and subjected to proteomic analysis. Stimulation with the vaccine resulted in 66 differentially expressed proteins associated with mRNA translation, immune responses, lipid metabolism and the proteasome. For the eight most significantly upregulated proteins, mRNA expression levels were assessed. Markers that showed increased expression at both mRNA and protein levels included PLIN2 and PSMB1. Stimulation with infectious bronchitis virus only resulted in 25 differentially expressed proteins, which were mostly proteins containing Src homology 2 domains. Stimulation with lipopolysaccharides resulted in 118 differentially expressed proteins associated with dendritic cell maturation and antimicrobial activity. This study provides leads to a better understanding of the activation of dendritic cells by an inactivated poultry vaccine, and identified PLIN2 and PSMB1 as potential biomarkers for cell-based potency testing.

scholarly journals Comparing the innate and humoral immune responses of different chicken lines to Infectious Bronchitis Virus (IBV) infection

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Isaac Dowell ◽  
Angela Steyn ◽  
Sarah Keep ◽  
Erica Bickerton ◽  
John Hammond
Keyword(s):  
Viral Load ◽  
Immune Responses ◽  
Infectious Bronchitis Virus ◽  
Ciliary Activity ◽  
Infectious Bronchitis ◽  
Humoral Immune ◽  
Qrt Pcr ◽  
Commercial Chicken ◽  
Significant Difference ◽  
White Leghorns

Infectious Bronchitis Virus (IBV) is a gammacoronavirus that is prevalent in commercial chicken flocks, resulting in characteristic clinical signs including snicking, rales, decreased tracheal ciliary activity, reduced weight gain and reduced egg production. Preliminary results indicate that there is a different clinical response to IBV infection in different chicken lines. Therefore, we aim to determine whether there is a differential innate or humoral immune response to IBV between chicken lines. A series of in vivo experiments were conducted comparing brown leghorns (Rhode Island Red, RIR (Roslin)) to white leghorns (from Valo and Ovagen). Trachea and bursa were collected from infected and control birds at four-, six- and fourteen-days post infection (dpi). There was a difference in snick rate and rales between the RIRs and the white leghorns (both lines). However, no difference was observed in ciliary activity. Viral load was determined by absolute quantification using qRT-PCR. The viral load in the trachea of RIRs was significantly lower (p<0.05) at 6 dpi compared to 4 dpi, unlike in Ovagen birds where there was no significant difference between the timepoints. Relative gene expression of IFN-α, IFN-β, IFN-γ, IL-6 and IL-1β in these tissues will be measuredby qRT-PCR. Serum was processed from whole blood collected at zero and ten dpi for use in IBV specific ELISAs which will measure antibody responses in the chicken lines. This project aims to explore immune responses against IBV as well as identifying the causes of variability in experimentation using chickens to investigate IBV infection.

Inflammatory and Cell-Mediated Immune Responses in the Respiratory Tract of Chickens to Infection with Avian Infectious Bronchitis Virus

2014 ◽  
Vol 27 (8) ◽  
pp. 383-391 ◽  
Author(s):  
Cintia Hiromi Okino ◽  
Igor Leonardo dos Santos ◽  
Filipe Santos Fernando ◽  
Antônio Carlos Alessi ◽  
Xiuqing Wang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Respiratory Tract ◽  
Infectious Bronchitis Virus ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis
Sign in / Sign up

Export Citation Format

Share Document