Protein Transfer Enhances Cellular Immune Responses to DNA Vaccination Against SARS-CoV

2009 ◽  
Vol 22 (6) ◽  
pp. 417-422 ◽  
Author(s):  
Youmin Kang ◽  
Aoshuang Chen ◽  
Bin Wang ◽  
Guoxing Zheng
Keyword(s):  
Immune Responses ◽  
Dna Vaccination ◽  
Protein Transfer ◽  
Cellular Immune Responses ◽  
Cellular Immune

Antibody and cellular immune responses following DNA vaccination and EHV-1 infection of ponies

2006 ◽  
Vol 111 (1-2) ◽  
pp. 81-95 ◽  
Author(s):  
G. Soboll ◽  
S.B. Hussey ◽  
J.M. Whalley ◽  
G.P. Allen ◽  
M.T. Koen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccination ◽  
Cellular Immune Responses ◽  
Cellular Immune

Priming of measles virus-specific humoral- and cellular-immune responses in macaques by DNA vaccination

Vaccine ◽  
2002 ◽  
Vol 20 (16) ◽  
pp. 2022-2026 ◽  
Author(s):  
Koert J. Stittelaar ◽  
Rik L. de Swart ◽  
Helma W. Vos ◽  
Geert van Amerongen ◽  
Nathalie Sixt ◽  
...  
Keyword(s):  
Immune Responses ◽  
Measles Virus ◽  
Dna Vaccination ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals Increased Immune Response Elicited by DNA Vaccination with a Synthetic gp120 Sequence with Optimized Codon Usage

1998 ◽  
Vol 72 (2) ◽  
pp. 1497-1503 ◽  
Author(s):  
Stefanie André ◽  
Brian Seed ◽  
Josef Eberle ◽  
Winfried Schraut ◽  
Andreas Bültmann ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Codon Usage ◽  
Dna Vaccination ◽  
Wild Type ◽  
Cellular Immune Responses ◽  
Gp120 Sequence ◽  
Parasitic Pathogens ◽  
Cellular Immune

ABSTRACT DNA vaccination elicits humoral and cellular immune responses and has been shown to confer protection against several viral, bacterial, and parasitic pathogens. Here we report that optimized codon usage of an injected DNA sequence considerably increases both humoral and cellular immune responses. We recently generated a synthetic human immunodeficiency virus type 1 gp120 sequence in which most wild-type codons were replaced with codons from highly expressed human genes (syngp120). In vitro expression of syngp120 is considerably increased in comparison to that of the respective wild-type sequence. In BALB/c mice, DNA immunization with syngp120 resulted in significantly increased antibody titers and cytotoxic T-lymphocyte reactivity, suggesting a direct correlation between expression levels and the immune response. Moreover, syngp120 is characterized byrev-independent expression and a low risk of recombination with viral sequences. Thus, synthetic genes with optimized codon usage represent a novel strategy to increase the efficacy and safety of DNA vaccination.

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