Comparison of Bovine Immune Responses to Affinity-Purified Bovine Herpesvirus-1 Antiidiotypes and Glycoproteins

1993 ◽  
Vol 6 (2) ◽  
pp. 109-117 ◽  
Author(s):  
D.J. ORTEN ◽  
W. XUE ◽  
S. VAN DRUNEN LITTEL-VAN DEN HURK ◽  
O.Y. ABDELMAGID ◽  
D.N. REDDY ◽  
...  
Keyword(s):  
Immune Responses ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

2001 ◽  
Vol 78 (4) ◽  
pp. 293-305 ◽  
Author(s):  
Praveen K. Gupta ◽  
Mohini Saini ◽  
L.K. Gupta ◽  
V.D.P. Rao ◽  
S.K. Bandyopadhyay ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Augmentation of cellular immune responses to bovine herpesvirus-1 glycoprotein D by vaccination with CpG-enhanced plasmid vectors

2002 ◽  
Vol 83 (12) ◽  
pp. 2973-2981 ◽  
Author(s):  
R. A. Pontarollo ◽  
L. A. Babiuk ◽  
R. Hecker ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Immune Responses ◽  
Lymphocyte Proliferation ◽  
Bovine Herpesvirus ◽  
Saline Control ◽  
Control Group ◽  
Glycoprotein D ◽  
Saline Control Group ◽  
Bovine Herpesvirus 1 ◽  
Ifn Γ ◽  
Herpesvirus 1

The potential of CpG-enhanced plasmid DNA vectors encoding a truncated secreted form of bovine herpesvirus-1 (BHV-1) glycoprotein D (tgD) to induce enhanced immune responses in cattle was investigated. We created tgD expression plasmids containing 0, 40 or 88 copies of the hexamer 5′ GTCGTT 3′, a known pan-activating CpG motif in several species. The total tgD-specific IgG titre of calves immunized with these plasmids did not correlate with the CpG content of the plasmid backbone. However, the pBISIA88-tgD-vaccinated group showed a significantly lower IgG1:IgG2 ratio than calves immunized with pBISIA40-tgD or pMASIA-tgD, which has no CpG motifs inserted. Antigen-specific lymphocyte proliferation and IFN-γ secretion by peripheral blood mononuclear cells correlated positively with the CpG content of the vectors. In contrast, calves that received a killed BHV-1 vaccine had an IgG1-predominant isotype and low lymphocyte proliferation and IFN-γ levels. Following challenge, the pBISIA88-tgD-immunized group developed the greatest anamnestic response, the highest BHV-1 neutralization titres in serum and a significantly lower level of virus shedding than the saline control group. However, there were no significant differences in clinical symptoms of infection between the DNA-immunized groups and the saline control group. These data indicate that CpG-enhanced plasmids induce augmented immune responses and could be used to vaccinate against pathogens requiring a strong cellular response for protection.

scholarly journals Immunization with a bovine herpesvirus 1 glycoprotein B DNA vaccine induces cytotoxic T-lymphocyte responses in mice and cattle

2005 ◽  
Vol 86 (4) ◽  
pp. 887-898 ◽  
Author(s):  
Y. Huang ◽  
L. A. Babiuk ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Cytotoxic T Lymphocyte ◽  
Bovine Herpesvirus ◽  
Plasmid Vector ◽  
Injection System ◽  
Glycoprotein B ◽  
Bovine Herpesvirus 1 ◽  
Ctl Response ◽  
Herpesvirus 1

Virus-specific cytotoxic T lymphocytes (CTLs) are considered to be important in protection against and recovery from viral infections. In this study, several approaches to induce cytotoxicity against bovine herpesvirus 1 (BHV-1) were evaluated. Vaccination of C57BL/6 mice with BHV-1 induced a strong humoral, but no CTL, response, which may be due to downregulation of major histocompatibility complex class I molecules. In contrast, vaccinia virus expressing glycoprotein B (gB) elicited a weaker antibody response, but strong cytotoxicity, in mice. As an approach to inducing both strong humoral and cellular immune responses, a plasmid vector was then used to express gB. Both antibody and CTL responses were induced by the plasmid encoding gB in C57BL/6 and C3H mice, regardless of the type of vector backbone. This demonstrated that DNA immunization induces a broad-based immune response to BHV-1 gB. Interestingly, removal of the membrane anchor, which resulted in secretion of gB from transfected cells, did not result in reduced cytotoxicity. Here, it is shown that, compared with the cell-associated counterpart, plasmid-encoded secreted protein may induce enhanced immune responses in cattle. Therefore, calves were immunized intradermally with pMASIAtgB, a plasmid encoding the secreted form of gB (tgB), using a needle-free injection system. This demonstrated that pMASIAtgB elicited both humoral responses and activated gamma interferon-secreting CD8+ CTLs, suggesting that a DNA vaccine expressing tgB induces a CTL response in the natural host of BHV-1.

scholarly journals Intranasal delivery of plasmids expressing bovine herpesvirus 1 gB/gC/gD proteins by polyethyleneimine magnetic beads activates long-term immune responses in mice

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Xing-Bo Liu ◽  
Guo-Wei Yu ◽  
Xin-Yu Gao ◽  
Jin-Long Huang ◽  
Li-Ting Qin ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Plasmid Dna ◽  
Magnetic Beads ◽  
Vaccine Development ◽  
Bovine Herpesvirus ◽  
Intranasal Route ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

Abstract Background DNA vaccine is one of the research hotspots in veterinary vaccine development. Several advantages, such as cost-effectiveness, ease of design and production, good biocompatibility of plasmid DNA, attractive biosafety, and DNA stability, are found in DNA vaccines. Methods In this study, the plasmids expressing bovine herpesvirus 1 (BoHV-1) gB, gC, and gD proteins were mixed at the same mass ratio and adsorbed polyethyleneimine (PEI) magnetic beads with a diameter of 50 nm. Further, the plasmid and PEI magnetic bead polymers were packaged into double carboxyl polyethylene glycol (PEG) 600 to use as a DNA vaccine. The prepared DNA vaccine was employed to vaccinate mice via the intranasal route. The immune responses were evaluated in mice after vaccination. Results The expression of viral proteins could be largely detected in the lung and rarely in the spleen of mice subjected to a vaccination. The examination of biochemical indicators, anal temperature, and histology indicated that the DNA vaccine was safe in vivo. However, short-time toxicity was observed. The total antibody detected with ELISA in vaccinated mice showed a higher level than PBS, DNA, PEI + DNA, and PBS groups. The antibody level was significantly elevated at the 15th week and started to decrease since the 17th week. The neutralizing antibody titer was significantly higher in DNA vaccine than naked DNA vaccinated animals. The total IgA level was much greater in the DNA vaccine group compared to other component vaccinated groups. The examination of cellular cytokines and the percentage of CD4/CD8 indicated that the prepared DNA vaccine induced a strong cellular immunity. Conclusion The mixed application of plasmids expressing BoHV-1 gB/gC/gD proteins by nano-carrier through intranasal route could effectively activate long-term humoral, cellular, and mucosal immune responses at high levels in mice. These data indicate PEI magnetic beads combining with PEG600 are an efficient vector for plasmid DNA to deliver intranasally as a DNA vaccine candidate.

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