Rearrangement and Expression of T-Cell Receptor Genes in Marmoset Lymphoid Cell Lines with Natural Killer Activity

1990 ◽  
Vol 3 (1) ◽  
pp. 9-17 ◽  
Author(s):  
MARIO STEVENSON ◽  
DONALD R. JOHNSON
Keyword(s):  
T Cell ◽  
Natural Killer ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Lymphoid Cell ◽  
Natural Killer Activity ◽  
Cell Receptor ◽  
Killer Activity ◽  
T Cell Receptor Genes ◽  
Lymphoid Cell Lines

scholarly journals Fetal liver T cell receptor gamma/delta+ T cells as cytotoxic T lymphocytes specific for maternal alloantigens.

1992 ◽  
Vol 176 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Y Miyagawa ◽  
T Matsuoka ◽  
A Baba ◽  
T Nakamura ◽  
T Tsuno ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Fetal Liver ◽  
Cell Receptor ◽  
Lymphoid Cells ◽  
Gamma Delta T Cells ◽  
Gamma Delta ◽  
Killer Activity

We have established fetal liver-derived T cell receptor (TCR) gamma/delta+, CD3+ T cell lines that are cytotoxic for maternal T cells. Fetal liver-derived lymphoid progenitors yielded predominantly TCR-gamma/delta+ cell clusters when cultured on fetal bone marrow-derived stromal cells in the presence of a cytokine cocktail under magnetic force. These tightly adherent clusters were cloned by limiting dilution and the resulting cell lines analyzed for phenotype and function. Six of eight TCR-gamma/delta lines from 8-9.5-wk gestation fetuses were V delta 2+ as compared with zero of eight lines from later stages of gestation (10 and 15 wk), where all the lines were V delta 1+. In cytotoxicity assays, these TCR-gamma/delta+, CD3+, CD4-, and CD8+ or CD8- long-term cultured lymphoid cells (LLC) were killer cells active against the class I antigens on maternal T cells. Of the cell lines, the CD8+ TCR-gamma/delta+ LLC had the highest levels of killer activity. Thus fetal liver TCR-gamma/delta+ T cells may play a crucial role in protection against invading maternal T cells generated in the feto-maternal interaction.

Analysis of Function, Specificity and T Cell Receptor Expression of Cloned Mucosal T Cell Lines in Crohn's Disease

1996 ◽  
Vol 9 (2) ◽  
pp. 193-204 ◽  
Author(s):  
Thomas P. Prindiville ◽  
Mary C. Cantrell ◽  
Takayuki Matsumoto ◽  
William R. Brown ◽  
Aftab A. Ansari ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
T Cell ◽  
Crohn's Disease ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Cell Receptor ◽  
Receptor Expression ◽  
T Cell Lines ◽  
Analysis Of Function
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