The Profile of Immunoglobulin A and Immunoglobulin G Subclasses in Sprague Dawley Rats Experimentally Infected with Brucella abortus Biotype 1

2020 ◽  
Vol 20 (5) ◽  
pp. 358-364
Author(s):  
Mst. Minara Khatun ◽  
Md. Ariful Islam ◽  
Byeong Kirl Baek
Keyword(s):  
Immunoglobulin G ◽  
Brucella Abortus ◽  
Immunoglobulin A ◽  
Sprague Dawley ◽  
Immunoglobulin G Subclasses ◽  
Sprague Dawley Rats

scholarly journals Whole tissue homogenization preferable to mucosal scraping in determining the temporal profile of segmented filamentous bacteria in the ileum of weanling rats

2021 ◽  
Vol 3 (3) ◽  
Author(s):  
Linda A. Oemcke ◽  
Rachel C. Anderson ◽  
Jasna Rakonjac ◽  
Warren C. McNabb ◽  
Nicole C. Roy
Keyword(s):  
Immunoglobulin A ◽  
Interleukin 17 ◽  
Filamentous Bacteria ◽  
Sprague Dawley ◽  
Suitable Alternative ◽  
Segmented Filamentous Bacteria ◽  
Sprague Dawley Rats ◽  
Weanling Rats ◽  
Ileal Tissue ◽  
Mucosal Scraping

Segmented filamentous bacteria (SFB) are thought to play a role in small intestine immunological maturation. Studies in weanling mice have shown a positive correlation between ileal SFB abundance and plasma and faecal interleukin 17 (IL-17) and immunoglobulin A (IgA) concentrations. Although the first observation of SFB presence was reported in rats, most studies use mice. The size of the mouse ileum is a limitation whereas the rat could be a suitable alternative for sufficient samples. Changes in SFB abundance over time in rats were hypothesized to follow the pattern reported in mice and infants. We characterized the profile of SFB colonization in the ileum tissue and contents and its correlation with two immune markers of gastrointestinal tract (GIT) maturation. We also compared two published ileum collection techniques to determine which yields data on SFB abundance with least variability. Whole ileal tissue and ileal mucosal scrapings were collected from 20- to 32-day-old Sprague-Dawley rats. SFB abundance was quantified from proximal, middle and distal ileal tissues, contents and faeces by quantitative PCR using SFB-specific primers. Antibody-specific ELISAs were used to determine IL-17 and IgA concentrations. Significant differences in SFB abundance were observed from whole and scraped tissues peaking at day 22. Variability in whole ileum data was less, favouring it as a better collection technique. A similar pattern of SFB abundance was observed in ileum contents and faeces peaking at day 24, suggesting faeces can be a proxy for ileal SFB abundance. SFB abundance at day 26 was higher in females than males across all samples. There were significant differences in IgA concentration between days 20, 30 and 32 and none in IL-17 concentration, which was different from reports in mice and infants.

scholarly journals Facilitated Intranasal Induction of Mucosal and Systemic Immunity to Mutans Streptococcal Glucosyltransferase Peptide Vaccines

2001 ◽  
Vol 69 (8) ◽  
pp. 4767-4773 ◽  
Author(s):  
Daniel J. Smith ◽  
William F. King ◽  
Leigh A. Barnes ◽  
Debra Trantolo ◽  
Donald L. Wise ◽  
...  
Keyword(s):  
Intranasal Administration ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Peptide Vaccines ◽  
Sprague Dawley ◽  
Igg Antibody ◽  
Serum Igg ◽  
Mucosal Antibody ◽  
Sprague Dawley Rats ◽  
Plga Microparticles

ABSTRACT Synthetic peptide vaccines which are derived from functional domains of Streptococcus mutans glucosyltransferases (GTF) have been shown to induce protective immunity in Sprague-Dawley rats after subcutaneous injection in the salivary gland region. Since mucosal induction of salivary immunity would be preferable in humans, we explored methods to induce mucosal antibody in the rat to the GTF peptide vaccines HDS and HDS-GLU after intranasal administration. Several methods of facilitation of the immune response were studied: the incorporation of peptides in bioadhesive poly(d,l-lactide-coglycolide) (PLGA) microparticles, the use of monoepitopic (HDS) or diepitopic (HDS-GLU) peptide constructs, or the use of mucosal adjuvants. Salivary immunoglobulin A (IgA) responses were not detected after intranasal administration of diepitopic HDS-GLU peptide constructs in alum or after incorporation into PLGA microparticles. However, significant primary and secondary salivary IgA and serum IgG antibody responses to HDS were induced in all rats when cholera holotoxin (CT) or a detoxified mutant Escherichia coli heat-labile enterotoxin (R192G LT) were intranasally administered with HDS peptide constructs in PLGA. Coadministration of LT with HDS resulted in predominantly IgG2a responses in the serum, while coadministration with CT resulted in significant IgG1 and IgG2a responses to HDS. Serum IgG antibody, which was induced to the HDS peptide construct by coadministration with these adjuvants, also bound intact mutans streptococcal GTF in an enzyme-linked immunosorbent assay and inhibited its enzymatic activity. Thus, immune responses which are potentially protective for dental caries can be induced to peptide-based GTF vaccines after mucosal administration if combined with the CT or LT R192G mucosal adjuvant.

scholarly journals Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella Typhimurium

2015 ◽  
Vol 4 (3) ◽  
pp. 1 ◽  
Author(s):  
. Nurliyani ◽  
Madarina Julia ◽  
Eni Harmayani ◽  
Muthi Ikawati ◽  
Endang Baliarti
Keyword(s):  
Lactobacillus Plantarum ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Immunoglobulin A ◽  
Fermented Milk ◽  
Mucosal Immune Response ◽  
Soluble Fiber ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Secretory Immunoglobulin

<p><em>Lactobacillus plantarum</em> Dad-13 that isolated from “dadih” (traditional Indonesian fermented milk) has been known as probiotic, while sweet potato fiber has been proven as an effective prebiotic. The objective of this study was to evaluate the potency of <em>Lactobacillus plantarum</em> Dad-13 and sweet potato fiber as immunomodulators in terms of intestinal secretory immunoglobulin A (sIgA) and splenocyte gamma-interferon (IFN-?). Sixty male Sprague Dawley rats (uninfected and infected) were divided into five groups: AIN-93, Indonesian children diet (ICD), Sweet potato fiber (SPF), SPF + <em>Lactobacillus</em> <em>plantarum</em> Dad-13 (SPFL), and fructooligosaccharides + Lacto-B (FOSL). After diet intervention, the rats were killed and sampled including intestinal fluid, spleen and caecal digesta. The results showed that soluble fiber such as sweet potato fiber could not increase the number of lactobacilli in infected rats<em>, </em>but<em> </em>could play a role in mucosal immune response through the increasing of sIgA. While, <em>Lactobacillus plantarum</em> Dad-13 contained in the combination with sweet potato fiber may has potency in systemic immune stimulation, because of the tendency to increase level of splenocyte IFN-?<sub> </sub>in infected rats.</p>

scholarly journals Plate and Tube Agglutination Tests for Diagnosis of Brucella abortus Biotype 1 Infection in Sprague-Dawley Rats

1970 ◽  
Vol 2 (1) ◽  
pp. 63-67 ◽  
Author(s):  
MS Rahman
Keyword(s):  
Antibody Titer ◽  
Brucella Abortus ◽  
Post Infection ◽  
Physiological Saline ◽  
Potential Candidate ◽  
Sprague Dawley ◽  
Sd Rats ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B

The plate and tube agglutination tests were evaluated for the diagnosis of experimentally induced Brucella abortus biotype 1 infection in 45 female, 6 to 10 months old Sprague- Dawley (SD) rats during the period from 2001 to 2002. These 45 rats were divided into two groups A and B, of which group A consisting of 27 rats used for experimental infection, whereas 18 rats of group B served as uninfected control. Each rat of group A was injected subcutaneously @ 1.0×109 colony forming units (CFU) in 500 µl of bovine pathogenic strain of B. abortus biotype 1 suspension in physiological saline. The SD rats were monitored at regular intervals by serological and bacteriological methods. The reciprocal antibody titer was 1:400 through tube agglutination test (TAT) whereas it was 1:800 through plate agglutination (PAT) at first week of post-infection. There was no reciprocal antibody titer in sera of 24 weeks of post-infection both through PAT and TAT despite the presence of bacteremia and these tests were evaluated for the first time using sera from rat with brucellosis. PAT using B. abortus strain 1119-3 (S1119-3) whole cell antigen was a potential candidate as an improved diagnostic method for field diagnosis of brucellosis in wild animals. Key words: B. abortus biotype 1; plate and tube agglutination tests; Sprague-Dawley rats doi: 10.3329/bjvm.v2i1.1938 Bangl. J. Vet. Med. (2004). 2 (1) : 63-67

Serum immunoglobulin G subclasses and serum immunoglobulin A in acute bronchiolitis in infants

1993 ◽  
Vol 4 (1) ◽  
pp. 20-25 ◽  
Author(s):  
K. -H. Carlsen ◽  
O. J. Mellbye ◽  
P. Fuglerud ◽  
B. Johansen ◽  
A. B. Solheim ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Serum Immunoglobulin ◽  
Acute Bronchiolitis ◽  
Immunoglobulin G Subclasses

scholarly journals Analysis of Intestinal Mucosal Immunoglobulin A in Sprague Dawley Rats Supplemented with Tempeh

2015 ◽  
Vol 22 (1) ◽  
pp. 48-52
Author(s):  
SUSAN SOKA ◽  
ANTONIUS SUWANTO ◽  
IMAN RUSMANA ◽  
DONDIN SAJUTHI ◽  
DIAH ISKANDRIATI ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

Effects of Brucella abortus Biotype 1 Infection on the Reproductive Performance of Sprague-Dawley Rats

2009 ◽  
Vol 12 (4) ◽  
pp. 353-359 ◽  
Author(s):  
Md. Ariful Isl ◽  
Mst. Minara Kha ◽  
Byeong Kirl Baek ◽  
Sung Il Lee
Keyword(s):  
Reproductive Performance ◽  
Brucella Abortus ◽  
Sprague Dawley ◽  
Sprague Dawley Rats
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