A Serological Survey ofBorrelia burgdorferiInfection in Sheep in Northeast China Regions Through Outer Surface Protein C-Based Enzyme-Linked Immunosorbent Assay

2019 ◽  
Vol 19 (1) ◽  
pp. 16-21
Author(s):  
Xunliang Li ◽  
Pengchong Li ◽  
Tongyuan Zhang ◽  
Pengkun Zhang ◽  
Xiaofeng Ren ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Outer Surface ◽  
Northeast China ◽  
Protein C ◽  
Surface Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Outer Surface Protein

scholarly journals C-Terminal Region of Outer Surface Protein C Binds Borreliacidal Antibodies in Sera from Patients with Lyme Disease

2003 ◽  
Vol 10 (4) ◽  
pp. 573-578 ◽  
Author(s):  
Dean A. Jobe ◽  
Steven D. Lovrich ◽  
Ronald F. Schell ◽  
Steven M. Callister
Keyword(s):  
Lyme Disease ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carboxy Terminus ◽  
Outer Surface Protein ◽  
Serum Titer ◽  
High Concentrations

ABSTRACT Borreliacidal antibodies specific for outer surface protein C (OspC) are induced shortly after infection with Borrelia burgdorferi. In this study, we identified the region of OspC recognized by immunoglobulin M (IgM) and IgG borreliacidal antibodies. Sera from patients with early Lyme disease were screened for borreliacidal activity specific for B. burgdorferi 50772 and OspC antibodies. Seven sera that contained similarly high titers of each response were then chosen randomly and adsorbed with OspC or a truncated OspC (OspC-Dra) containing the 50 amino acids nearest the carboxy terminus. Adsorption with OspC or OspC-Dra completely eliminated the borreliacidal activity in six (86%) of seven sera and significantly decreased the activity in the remaining serum (titer of 10,240 to 1,280). Moreover, OspC antibodies were no longer detected by OspC enzyme-linked immunosorbent assay or in a Western blot that contained native OspC. The findings confirmed that sera from patients with early Lyme disease contain high concentrations of IgM or IgG borreliacidal antibodies that bind a conserved region of OspC.

scholarly journals Antibody response to OspC-I synthetic peptide derived from outer surface protein C of Borrelia burgdorferi in sera from Japanese forestry workers

1999 ◽  
Vol 122 (3) ◽  
pp. 429-433 ◽  
Author(s):  
M. IKUSHIMA ◽  
F. YAMADA ◽  
S. KAWAHASHI ◽  
Y. OKUYAMA ◽  
K. MATSUI
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Synthetic Peptide ◽  
Protein C ◽  
Surface Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Forestry Workers ◽  
Outer Surface Protein

The prevalence of antibodies against Lyme disease spirochaetes in serum samples from 80 forestry workers at high occupational risk of Lyme disease was surveyed by enzyme-linked immunosorbent assay (ELISA) with the OspC-I synthetic peptide. The peptide is part of the outer surface protein C (OspC) amino acid sequence located in the region conserved among Borrelia burgdorferi sensu stricto or sensu lato. Positivity for antibodies against OspC-I was observed in 25 (31·3%) of the forestry workers. Of these positive cases, 12 (15·0%) and 19 (23·8%) were positive for immunoglobulin M (IgM) and IgG antibody, respectively. Among 62 workers who were negative for IgG antibody against B. garinii or B. japonica in our previous study, 9 (14·5%) and 4 (6·5%) were positive for IgM and IgG antibody, respectively, in OspC-I ELISA. These results demonstrate for the first time that Lyme disease in forestry workers can be revealed using OspC-I ELISA. We conclude that forestry workers who show positive results for antibodies against OspC-I have very likely been exposed to Lyme disease spirochaetes, and that those who show positivity for IgM antibody against OspC-I may be in the early stage of Lyme disease.

scholarly journals Strain-specific joint invasion and colonization by Lyme disease spirochetes is promoted by outer surface protein C

2020 ◽  
Vol 16 (5) ◽  
pp. e1008516 ◽  
Author(s):  
Yi-Pin Lin ◽  
Xi Tan ◽  
Jennifer A. Caine ◽  
Mildred Castellanos ◽  
George Chaconas ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Outer Surface Protein

scholarly journals Constitutive Expression of Outer Surface Protein C Diminishes the Ability of Borrelia burgdorferi To Evade Specific Humoral Immunity

2006 ◽  
Vol 74 (9) ◽  
pp. 5177-5184 ◽  
Author(s):  
Qilong Xu ◽  
Sunita V. Seemanapalli ◽  
Kristy McShan ◽  
Fang Ting Liang
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein C ◽  
Severe Combined Immunodeficiency ◽  
Constitutive Expression ◽  
Surface Protein ◽  
Start Codon ◽  
Chronic Infections ◽  
Translational Initiation ◽  
Outer Surface Protein

ABSTRACT The Lyme disease spirochete Borrelia burgdorferi reduces the expression of outer surface protein C (OspC) in response to the development of an anti-OspC humoral response, leading to the hypothesis that the ability to repress OspC expression is critical for the pathogen to proceed to chronic infection. B. burgdorferi was genetically modified to constitutively express OspC by introducing an extra ospC copy fused with the borrelial flagellar gene (flaB) promoter. Such a genetic modification did not reduce infectivity or pathogenicity in severe combined immunodeficiency mice but resulted in clearance of infection by passively transferred OspC antibody. Spirochetes with constitutive ospC expression were unable to establish chronic infections in immunocompetent mice unless they had undergone very destructive mutations in the introduced ospC copy. Two escape mutants were identified; one had all 7 bp deleted between the putative ribosome-binding site and the start codon, ATG, causing a failure in translational initiation, and the other mutant had an insertion of 2 bp between nucleotides 315 and 316, resulting in a nonsense mutation at codon 108. Thus, the ability of B. burgdorferi to repress ospC expression during mammalian infection allows the pathogen to avoid clearance and to preserve the integrity of the important gene for subsequent utilization during its enzootic life cycle.

scholarly journals Outer Surface Protein C Is a Dissemination-Facilitating Factor of Borrelia burgdorferi during Mammalian Infection

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15830 ◽  
Author(s):  
Sunita V. Seemanapalli ◽  
Qilong Xu ◽  
Kristy McShan ◽  
Fang Ting Liang
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Protein C ◽  
Surface Protein ◽  
Outer Surface Protein ◽  
Mammalian Infection
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