scholarly journals Genomic Characterization of Crimean–Congo Hemorrhagic Fever Virus inHyalommaTick from Spain, 2014

2017 ◽  
Vol 17 (10) ◽  
pp. 714-719 ◽  
Author(s):  
Maria N.B. Cajimat ◽  
Sergio E. Rodriguez ◽  
Isolde U.E. Schuster ◽  
Daniele M. Swetnam ◽  
Thomas G. Ksiazek ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Genomic Characterization

Molecular characterization of small and medium segments of Crimean-Congo hemorrhagic fever virus in Turkey

2020 ◽  
Vol 15 (4) ◽  
pp. 247-254
Author(s):  
Murat Karamese ◽  
Erkan Ozmen ◽  
Hakan Aydin ◽  
Mehmet Ozkan Timurkan ◽  
Mesud Fakirullahoglu
Keyword(s):  
Infectious Disease ◽  
Phylogenetic Analysis ◽  
Phylogenetic Tree ◽  
Molecular Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Relationship Of

Aim: The objective was to investigate the genotypic relationship of S and M segments in Crimean-Congo hemorrhagic fever virus (CCHFV) by phylogenetic analysis in 25 patients from seven endemic cities in Turkey. Materials & methods: A total of 25 samples from patients with CCHF were included between 2012 and 2015. Phylogenetic tree analyses were inferred using MEGA version-6.0 and distances were calculated by Kimura’s 2-parameter. Results: Phylogenetic analysis showed that all isolated viruses (n = 25) were in the predicted clades such as clade V- Europe-1 regarding both S and M segments of the CCHFV. Conclusion: Further epidemiological, molecular and phylogenic studies should be performed in both reservoir animals/vectors and humans to determine the incidence of tick-borne infectious disease and to help to develop vaccines for prevention of the disease.

scholarly journals Genetic characterization of Erve virus, a European Nairovirus distantly related to Crimean-Congo hemorrhagic fever virus

Virus Genes ◽  
2012 ◽  
Vol 45 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Meik Dilcher ◽  
Andrea Koch ◽  
Lekbira Hasib ◽  
Gerhard Dobler ◽  
Frank T. Hufert ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

Genetic Characterization of the M RNA Segment of Crimean-Congo Hemorrhagic Fever Virus Strains Isolated in Russia and Tajikistan

Virus Genes ◽  
2004 ◽  
Vol 28 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Sergei V. Seregin ◽  
Evgeny I. Samokhvalov ◽  
Irina D. Petrova ◽  
Oleg I. Vyshemirskii ◽  
Ekaterina G. Samokhvalova ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Strains

scholarly journals Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain

2017 ◽  
Vol 23 (12) ◽  
pp. 2078-2080 ◽  
Author(s):  
Eva Ramírez de Arellano ◽  
Lourdes Hernández ◽  
M. José Goyanes ◽  
Marta Arsuaga ◽  
Ana Fernández Cruz ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Phylogenetic Characterization ◽  
Hemorrhagic Fever Virus

scholarly journals Dual RNA-Seq characterization of host and pathogen gene expression in liver cells infected with Crimean-Congo Hemorrhagic Fever Virus

2020 ◽  
Vol 14 (4) ◽  
pp. e0008105
Author(s):  
Robert A. Kozak ◽  
Russell S. Fraser ◽  
Mia J. Biondi ◽  
Anna Majer ◽  
Sarah J. Medina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hemorrhagic Fever ◽  
Liver Cells ◽  
Fever Virus ◽  
Rna Seq ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

scholarly journals Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus Detected in African Blue Ticks Feeding on Cattle in a Ugandan Abattoir

2021 ◽  
Vol 9 (2) ◽  
pp. 438
Author(s):  
Eddie M. Wampande ◽  
Peter Waiswa ◽  
David J. Allen ◽  
Roger Hewson ◽  
Simon D. W. Frost ◽  
...  
Keyword(s):  
Sequence Data ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Western India ◽  
Coding Region ◽  
Crimean Congo Hemorrhagic Fever ◽  
Synonymous Mutations ◽  
Hemorrhagic Fever Virus ◽  
Genotype Ii

Crimean-Congo hemorrhagic fever virus (CCHFV) is the most geographically widespread of the tick-borne viruses. However, African strains of CCHFV are poorly represented in sequence databases. In addition, almost all sequence data collected to date have been obtained from cases of human disease, while information regarding the circulation of the virus in tick and animal reservoirs is severely lacking. Here, we characterize the complete coding region of a novel CCHFV strain, detected in African blue ticks (Rhipicephalus (Boophilus) decoloratus) feeding on cattle in an abattoir in Kampala, Uganda. These cattle originated from a farm in Mbarara, a major cattle-trading hub for much of Uganda. Phylogenetic analysis indicates that the newly sequenced strain belongs to the African genotype II clade, which predominantly contains the sequences of strains isolated from West Africa in the 1950s, and South Africa in the 1980s. Whilst the viral S (nucleoprotein) and L (RNA polymerase) genome segments shared >90% nucleotide similarity with previously reported genotype II strains, the glycoprotein-coding M segment shared only 80% nucleotide similarity with the next most closely related strains, which were derived from ticks in Western India and Northern China. This genome segment also displayed a large number of non-synonymous mutations previously unreported in the genotype II strains. Characterization of this novel strain adds to our limited understanding of the natural diversity of CCHFV circulating in both ticks and in Africa. Such data can be used to inform the design of vaccines and diagnostics, as well as studies exploring the epidemiology and evolution of the virus for the establishment of future CCHFV control strategies.

scholarly journals Neutralizing Antibodies against Crimean–Congo Hemorrhagic Fever Virus Derived from a Human Survivor

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 128
Author(s):  
J. Maximilian Fels ◽  
Daniel Maurer ◽  
Ana I. Kuehne ◽  
Dafna M. Abelson ◽  
Noel T. Pauli ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Hemorrhagic Fever ◽  
Reservoir Host ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Isolation And Characterization ◽  
Western Asia ◽  
Targeted Treatments

Crimean–Congo hemorrhagic fever virus (CCHFV) is an arbovirus belonging to the Nairoviridae family. The virus, as well as ticks of the Hyalomma genus, which serve as its reservoir host, are found in parts of Africa, western Asia, and southern Europe. Following sporadic zoonotic or human-to-human transmission, infection is characterized by fever, fatigue, vomiting, diarrhea, and in fatal cases, often hemorrhagic symptoms. There are currently no vaccines or targeted treatments available against CCHFV, leading the WHO to declare it a Blueprint priority pathogen in 2017. Here, we report the isolation and characterization of a panel of human monoclonal antibodies (mAbs) against CCHFV. Using a novel soluble Gn/Gc sorting antigen, we were able to isolate memory B cells specific for CCHFV from four convalescent donors. From each patient sample, we were able to derive several potently neutralizing antibodies with IC50 in the nanomolar range as determined by neutralization of CCHFV virus-like particles. Neutralization by candidate hits was also confirmed using authentic CCHFV. We further show that several of the most potently neutralizing mAbs possess a breadth of neutralization spanning three clades of CCHFV strains. These broadly neutralizing mAbs are currently being tested in a mouse model of CCHFV infection, with preliminary results indicating that they have protective potential.

Sign in / Sign up

Export Citation Format

Share Document