Dengue Virus Isolation in Mosquito Aedes albopictus Captured During an Outbreak in Tokyo, 2014, by a Method Relying on Antibody-Dependent Enhancement Mechanism Using FcγR-Expressing BHK Cells

2016 ◽  
Vol 16 (12) ◽  
pp. 810-812 ◽  
Author(s):  
Meng Ling Moi ◽  
Daisuke Kobayashi ◽  
Haruhiko Isawa ◽  
Toshinori Sasaki ◽  
Masayuki Saijo ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Virus Isolation ◽  
Antibody Dependent Enhancement ◽  
Bhk Cells ◽  
Enhancement Mechanism

scholarly journals Aedes albopictus may not be vector of dengue virus in human epidemics in Brazil

2003 ◽  
Vol 37 (3) ◽  
pp. 386-387 ◽  
Author(s):  
Nicolas Degallier ◽  
José Marcus Sócrates Teixeira ◽  
Sidinei da Silva Soares ◽  
Regilene D Pereira ◽  
Santuzza C F Pinto ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Virus Isolation ◽  
The State ◽  
Serological Testing ◽  
Serum Samples ◽  
Study Results ◽  
Elisa Testing ◽  
Espirito Santo ◽  
Dengue Epidemics

Over 60,500 dengue cases were reported in the state of Espírito Santo (ES), Brazil, between 1995 and 1998. The study's purpose was to identify whether Aedes albopictus was transmitting the dengue virus during an epidemic in the locality of Vila Bethânia (Viana County),Vitória, ES. From April 3 to 9, 1998, blood and serum samples were collected daily for virus isolation and serological testing. Four autochthonous cases were confirmed through DEN 1 virus isolation and two autochthonous cases through MAC ELISA testing. Of 37 Ae. aegypti and 200 Ae. albopictus adult mosquitoes collected and inoculated, DEN1 virus was isolated only from a pool of two Ae. aegypti female mosquitoes. The study results suggest that Ae. albopictus still cannot be considered an inter-human vector in dengue epidemics in Brazil.

scholarly journals Virological confirmation of concurrent dengue virus serotypes 1 and 4 by virus isolation using Fc-gamma receptor-expressing BHK cells

2015 ◽  
Vol 33 ◽  
pp. 177-178 ◽  
Author(s):  
Meng Ling Moi ◽  
Yasuko Honma ◽  
Satomi Mori ◽  
Toshiki Kuze ◽  
Masumi Kanekawa ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Isolation ◽  
Fc Gamma Receptor ◽  
Bhk Cells ◽  
Gamma Receptor ◽  
Dengue Virus Serotypes

scholarly journals Transcriptome Analysis of Responses to Dengue Virus 2 Infection in Aedes albopictus (Skuse) C6/36 Cells

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 343
Author(s):  
Manjin Li ◽  
Dan Xing ◽  
Duo Su ◽  
Di Wang ◽  
Heting Gao ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Bioinformatics Analysis ◽  
Interaction Mechanism ◽  
Transcriptional Analysis ◽  
Functional Verification ◽  
Mosquito Vector ◽  
Rna Seq ◽  
Sequencing Data ◽  
Qrt Pcr

Dengue virus (DENV), a member of the Flavivirus genus of the Flaviviridae family, can cause dengue fever (DF) and more serious diseases and thus imposes a heavy burden worldwide. As the main vector of DENV, mosquitoes are a serious hazard. After infection, they induce a complex host–pathogen interaction mechanism. Our goal is to further study the interaction mechanism of viruses in homologous, sensitive, and repeatable C6/36 cell vectors. Transcriptome sequencing (RNA-Seq) technology was applied to the host transcript profiles of C6/36 cells infected with DENV2. Then, bioinformatics analysis was used to identify significant differentially expressed genes and the associated biological processes. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to verify the sequencing data. A total of 1239 DEGs were found by transcriptional analysis of Aedes albopictus C6/36 cells that were infected and uninfected with dengue virus, among which 1133 were upregulated and 106 were downregulated. Further bioinformatics analysis showed that the upregulated DEGs were significantly enriched in signaling pathways such as the MAPK, Hippo, FoxO, Wnt, mTOR, and Notch; metabolic pathways and cellular physiological processes such as autophagy, endocytosis, and apoptosis. Downregulated DEGs were mainly enriched in DNA replication, pyrimidine metabolism, and repair pathways, including BER, NER, and MMR. The qRT-PCR results showed that the concordance between the RNA-Seq and RT-qPCR data was very high (92.3%). The results of this study provide more information about DENV2 infection of C6/36 cells at the transcriptome level, laying a foundation for further research on mosquito vector–virus interactions. These data provide candidate antiviral genes that can be used for further functional verification in the future.

scholarly journals Entomological Status Based on Vector Density Index and Transovarial Infection on Aedes Sp. Mosquito in Meteseh Village, Semarang City

2020 ◽  
Vol 202 ◽  
pp. 12016
Author(s):  
Martini Martini ◽  
Zufri Armen ◽  
Kusariana Nissa ◽  
Hestiningsih Retno ◽  
Yuliawati Sri ◽  
...  
Keyword(s):  
Health Services ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Research Method ◽  
Hemorrhagic Fever ◽  
Adult Mosquito ◽  
Elisa Method ◽  
Density Index ◽  
Egg Density ◽  
Descriptive Method

Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue virus. In 2016, the number of DHF patients in areas of Health services of Rowosari was 247 cases, IR 289.6/100.000 populations and the highest number of the patients was from Meteseh Village. The most usual mechanism happened in the vertical transmission, transovarial infection, from adult mosquito to the egg. The study was conducted to describe of entomological status of House Index (HI), Container Index (CI), Breteau Index (BI), House index (HI), Ovitrap Index (OI) of larvae as well as transovarial infection in Aedes sp. The research method was descriptive method by conducting test to the entomology status of DHF incidence. Based on the survey in Meteseh sub-village (XVI), a result found that average House Index 8.99%, Container Index 2.88%, Breteau Index 16.40%, HI 8,99 Ovitrap Index 35.46% with the egg density at 1.023 eggs. The identified mosquitoes were 30, and from the identification result of Aedes sp., two Aedes species were found, 28 Aedes aegypti,and only 2 Aedes albopictus. The transovarial infection in Aedes sp. using ELISA method was found 6 mosquito samples which were positive of dengue virus, with the detail in neighborhood 01 (1 sample), neighborhood 02 (2 samples), neighborhood 03 (3 samples). DHF in Meteseh Sub village in Semarang City was probably happened because correlated with transovarial infection.

Virus Isolation for Diagnosing Dengue Virus Infections in Returning Travelers

2003 ◽  
Vol 22 (11) ◽  
pp. 697-700 ◽  
Author(s):  
D. Teichmann ◽  
K. G�bels ◽  
M. Niedrig ◽  
J.-W. Sim-Brandenburg ◽  
J. L�ge-Stehr ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Isolation ◽  
Virus Infections
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