Genome Survey Sequence Analysis and Identification of Homologs of Major Surface Protease (gp63) Genes inTrypanosoma rangeli

2010 ◽  
Vol 10 (9) ◽  
pp. 847-853 ◽  
Author(s):  
Keila A.M. Ferreira ◽  
Jeronimo C. Ruiz ◽  
Fabrício C. Dias ◽  
Eliane Lages-Silva ◽  
Luiz R.O. Tosi ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genome Survey Sequence ◽  
Genome Survey ◽  
Survey Sequence ◽  
Major Surface

scholarly journals Detection of Cattle Naturally Infected with Anaplasma marginale in a Region of Endemicity by Nested PCR and a Competitive Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5

1998 ◽  
Vol 36 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Susana Torioni de Echaide ◽  
Donald P. Knowles ◽  
Travis C. McGuire ◽  
Guy H. Palmer ◽  
Carlos E. Suarez ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Immunosorbent Assay ◽  
Nested Pcr ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
Epidemiological Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Surface Protein ◽  
Persistent Infections ◽  
Major Surface

A competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5 (rMSP5-cELISA) of Anaplasma marginale was validated in a naturally infected cattle herd in an area of eastern Oregon where A. marginale is endemic. The true positive and negative A. marginale infection status of 235 randomly selected cattle was determined by using a nested PCR (nPCR) coupled with msp5 sequence analysis and hybridization. Judgment of the reliability of the nPCR and hybridization for detection of persistent infections was based on three observations. First, the nPCR was able to detect as few as 30 infected erythrocytes per ml. Second, the nPCR was able to consistently detect low levels of rickettsemia in seven carrier cattle experimentally infected with A. marginale. Third, msp5sequence analysis showed >95% identity among 30 nPCR amplicons from cattle naturally infected with field strains of A. marginale. The nPCR and hybridization identified 151 infected and 84 uninfected cattle among the 235 animals tested. With a cutoff point of 28%, the rMSP5-cELISA showed a sensitivity of 96% and a specificity of 95%. These results indicate that the rMSP5-cELISA can sensitively and specifically detect cattle with naturally acquired persistent A. marginale infections and suggest that it is an excellent assay for epidemiological studies, eradication programs, and regulation of international cattle movement.

scholarly journals Computational Tools forBrassica–ArabidopsisComparative Genomics

2005 ◽  
Vol 6 (3) ◽  
pp. 147-152 ◽  
Author(s):  
Paul Beckett ◽  
Ian Bancroft ◽  
Martin Trick
Keyword(s):  
Genome Sequence ◽  
In Silico ◽  
Physical Map ◽  
Genome Structure ◽  
Genome Project ◽  
Genome Survey ◽  
Physical Maps ◽  
Alignment Tool ◽  
Wide Range ◽  
Survey Sequence

Recent advances, such as the availability of extensive genome survey sequence (GSS) data and draft physical maps, are radically transforming the means by which we can dissectBrassicagenome structure and systematically relate it to theArabidopsismodel. Hitherto, our view of the co-linearities between these closely related genomes had been largely inferred from comparative RFLP data, necessitating substantial interpolation and expert interpretation. Sequencing of theBrassica rapagenome by the MultinationalBrassicaGenome Project will, however, enable an entirely computational approach to this problem. Meanwhile we have been developing databases and bioinformatics tools to support our work inBrassicacomparative genomics, including a recently completed draft physical map ofB. rapaintegrated with anchor probes derived from theArabidopsisgenome sequence. We are also exploring new ways to display the emergingBrassica–Arabidopsissequence homology data. We have mapped all publicly available Brassica sequencesin silicoto theArabidopsisTIGR v5 genome sequence and published this in the ATIDB database that uses Generic Genome Browser (GBrowse). Thisin silicoapproach potentially identifies all paralogous sequences and so we colour-code the significance of the mappings and offer an integrated, real-time multiple alignment tool to partition them into paralogous groups. The MySQL database driving GBrowse can also be directly interrogated, using the powerful API offered by the Perl Bio∷DB∷GFF methods, facilitating a wide range of data-mining possibilities.

Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region

1999 ◽  
Vol 37 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Y. GRAser ◽  
M. EL Fari ◽  
R. Vilgalys ◽  
A. F. A. Kuijpers ◽  
G. S. DE Hoog ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Its Region ◽  
The Family
Sign in / Sign up

Export Citation Format

Share Document