Evaluation of Recombinant Line Immunoblot for Detection of Lyme Disease in Slovakia: Comparison with Two Other Immunoassays

Daniela Lenčáková; Volker Fingerle; Astéria Štefančíková; Ulrike Schulte-Spechtel; Branislav Peťko; Ivan Schréter; Bettina Wilske

doi:10.1089/vbz.2007.0216.a

Evaluation of Recombinant Line Immunoblot for Detection of Lyme Disease in Slovakia: Comparison with Two Other Immunoassays

Vector-Borne and Zoonotic Diseases ◽

10.1089/vbz.2007.0216.a ◽

2008 ◽

Vol 8 (3) ◽

pp. 381-390 ◽

Cited By ~ 5

Author(s):

Daniela Lenčáková ◽

Volker Fingerle ◽

Astéria Štefančíková ◽

Ulrike Schulte-Spechtel ◽

Branislav Peťko ◽

...

Keyword(s):

Lyme Disease ◽

Recombinant Line

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New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160467 ◽

1990 ◽

Vol 48 (3) ◽

pp. 582-583

Author(s):

S. F. Hayes ◽

M. D. Corwin ◽

T. G. Schwan ◽

D. W. Dorward ◽

W. Burgdorfer

Keyword(s):

Lyme Disease ◽

Borrelia Burgdorferi ◽

Structural Characterization ◽

Negative Staining ◽

Low Speed ◽

Thin Sectioning ◽

Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

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