Cell Growth Dynamics in Embryonic and Adult Mouse Thyroid Revealed by a Novel Approach to Detect Thyroid Gland Subpopulations

Thyroid ◽  
2016 ◽  
Vol 26 (4) ◽  
pp. 591-599 ◽  
Author(s):  
Sanjay Gawade ◽  
Carlos Mayer ◽  
Katrin Hafen ◽  
Thomas Barthlott ◽  
Werner Krenger ◽  
...  
Keyword(s):  
Cell Growth ◽  
Thyroid Gland ◽  
Adult Mouse ◽  
Growth Dynamics ◽  
Novel Approach ◽  
Mouse Thyroid

scholarly journals Automated analysis of filopodial length and spatially resolved protein concentration via adaptive shape tracking

2016 ◽  
Vol 27 (22) ◽  
pp. 3616-3626 ◽  
Author(s):  
Tanumoy Saha ◽  
Isabel Rathmann ◽  
Abhiyan Viplav ◽  
Sadhana Panzade ◽  
Isabell Begemann ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Growth Dynamics ◽  
Automated Analysis ◽  
Cell Types ◽  
Control Mechanisms ◽  
Spatially Resolved ◽  
Novel Approach ◽  
Associated Proteins

Filopodia are dynamic, actin-rich structures that transiently form on a variety of cell types. To understand the underlying control mechanisms requires precise monitoring of localization and concentration of individual regulatory and structural proteins as filopodia elongate and subsequently retract. Although several methods exist that analyze changes in filopodial shape, a software solution to reliably correlate growth dynamics with spatially resolved protein concentration along the filopodium independent of bending, lateral shift, or tilting is missing. Here we introduce a novel approach based on the convex-hull algorithm for parallel analysis of growth dynamics and relative spatiotemporal protein concentration along flexible filopodial protrusions. Detailed in silico tests using various geometries confirm that our technique accurately tracks growth dynamics and relative protein concentration along the filopodial length for a broad range of signal distributions. To validate our technique in living cells, we measure filopodial dynamics and quantify spatiotemporal localization of filopodia-associated proteins during the filopodial extension–retraction cycle in a variety of cell types in vitro and in vivo. Together these results show that the technique is suitable for simultaneous analysis of growth dynamics and spatiotemporal protein enrichment along filopodia. To allow readily application by other laboratories, we share source code and instructions for software handling.

A model of cell growth dynamics

1967 ◽  
Vol 15 (2) ◽  
pp. 190-207 ◽  
Author(s):  
F.M. Williams
Keyword(s):  
Cell Growth ◽  
Growth Dynamics

scholarly journals A Novel Pharmacogenetic Model for Highly Efficient Ablation of Oligodendrocyte Progenitor Cells in the Adult Mouse CNS

2021 ◽  
Author(s):  
Yao Lulu Xing ◽  
Bernard H.A. Chuang ◽  
Jasmine Poh ◽  
Kaveh Moradi ◽  
Stanislaw Mitew ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Adult Mouse ◽  
Oligodendrocyte Progenitor Cells ◽  
Oligodendrocyte Progenitor ◽  
Cell Ablation ◽  
Novel Approach ◽  
Anterior Forebrain ◽  
Health And Disease ◽  
The Brain ◽  
Advance Knowledge

Approaches to investigate adult oligodendrocyte progenitor cells (OPCs) by targeted cell ablation in the rodent central nervous system have been limited by methodological challenges resulting in only partial and transient OPC depletion. We have developed a novel pharmacogenetic model of conditional OPC ablation, resulting in the elimination of 99.7% of all OPCs throughout the brain. By combining recombinase-based transgenic and viral strategies for targeting of OPCs and ventricular-subventricular zone (V-SVZ)-derived neural precursor cells (NPCs), we found that new PDGFRα-expressing cells born in the V-SVZ repopulated the OPC-deficient brain starting 12 days after OPC ablation. Our data reveal that OPC depletion induces V-SVZ-derived NPCs to generate vast numbers of PDGFRα+/NG2+ cells with the capacity to migrate and proliferate extensively throughout the dorsal anterior forebrain. Further application of this novel approach to ablate OPCs will advance knowledge of the function of both OPCs and oligodendrogenic NPCs in health and disease.

Dynamics of the nanocrystal structure and composition in growth solutions monitored by in situ lab-scale X-ray diffraction

Nanoscale ◽  
2021 ◽  
Author(s):  
Helena Fridman ◽  
Michael Volokh ◽  
Taleb Mokari
Keyword(s):  
Real Time ◽  
Growth Dynamics ◽  
X Ray Diffraction ◽  
X Ray ◽  
Nanocrystal Growth ◽  
Novel Approach ◽  
Phase Size ◽  
Time Observation ◽  
Real Time Observation

Nanocrystal growth dynamics are investigated by a novel approach: real-time observation of nanocrystals in growth solutions using lab-scale in situ X-ray diffraction. The method reveals the evolution of crystal phase, size, shape, and composition.

scholarly journals Mouse Thyroid Gland Changes in Aging: Implication of Galectin-3 and Sphingomyelinase

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
Giovanna Traina ◽  
Samuela Cataldi ◽  
Paola Siccu ◽  
Elisabetta Loreti ◽  
Ivana Ferri ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Older People ◽  
Thyroid Function ◽  
Thyroid Dysfunction ◽  
Structural Changes ◽  
Thyrotropin Receptor ◽  
Neutral Sphingomyelinase ◽  
Galectin 3 ◽  
Old Mice ◽  
Mouse Thyroid

Prevalence of thyroid dysfunction and its impact on cognition in older people has been demonstrated, but many points remain unclarified. In order to study the effect of aging on the thyroid gland, we compared the thyroid gland of very old mice with that of younger ones. We have first investigated the changes of thyroid microstructure and the possibility that molecules involved in thyroid function might be associated with structural changes. Results from this study indicate changes in the height of the thyrocytes and in the amplitude of interfollicular spaces, anomalous expression/localization of thyrotropin, thyrotropin receptor, and thyroglobulin aging. Thyrotropin and thyrotropin receptor are upregulated and are distributed inside the colloid while thyroglobulin fills the interfollicular spaces. In an approach aimed at defining the behavior of molecules that change in different physiopathological conditions of thyroid, such as galectin-3 and sphingomyelinase, we then wondered what was their behavior in the thyroid gland in aging. Importantly, in comparison with the thyroid of young animals, we have found a higher expression of galectin-3 and a delocalization of neutral sphingomyelinase in the thyroid of old animals. A possible relationship between galectin-3, neutral sphingomyelinase, and aging has been discussed.

THE ACTION OF DIBUTYRYL CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE ON THE MOUSE THYROID GLAND IN VITRO

1970 ◽  
Vol 47 (3) ◽  
pp. 333-338 ◽  
Author(s):  
PAT KENDALL-TAYLOR ◽  
D. S. MUNRO
Keyword(s):  
Thyroid Gland ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Adenosine Monophosphate ◽  
Thyroid Stimulating Hormone ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Layer Chromatography ◽  
Mouse Thyroid

SUMMARY The effects of dibutyryl cyclic 3′,5′-adenosine monophosphate (DBc-AMP) on the mouse thyroid gland have been investigated in the in-vitro assay of Brown & Munro (1967). The distribution of 131I-labelled compounds in the glands and the supporting medium have been analysed by thin-layer chromatography and the changes induced by cyclic 3′,5′-adenosine monophosphate (c-AMP), DBc-AMP or thyroid-stimulating hormone (TSH) compared. The release of 131I was increased when the glands were incubated with DBc-AMP, c-AMP or TSH. The potency of DBc-AMP was approximately 50 times that of c-AMP on a basis of molarity. Like TSH, DBc-AMP increased the proportion of iodothyronines in the system as a whole, whereas c-AMP had little effect. The possible explanations for this are discussed.

Iodine-125 Distribution between Follicular Colloid and Colloid Droplets in Mouse Thyroid Gland

Science ◽  
1964 ◽  
Vol 145 (3639) ◽  
pp. 1431-1432 ◽  
Author(s):  
W. C. Bauer ◽  
J. S. Meyer
Keyword(s):  
Thyroid Gland ◽  
Iodine 125 ◽  
Mouse Thyroid

scholarly journals Hes1 Is Required for Appropriate Morphogenesis and Differentiation during Mouse Thyroid Gland Development

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e16752 ◽  
Author(s):  
Aurore Carre ◽  
Latif Rachdi ◽  
Elodie Tron ◽  
Bénédicte Richard ◽  
Mireille Castanet ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Gland Development ◽  
Mouse Thyroid
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