scholarly journals Comparison of Thyroidectomized Calf Serum and Stripped Serum for the Study of Thyroid Hormone Action in Human Skin Fibroblasts In Vitro

Thyroid ◽  
2009 ◽  
Vol 19 (6) ◽  
pp. 639-644 ◽  
Author(s):  
Lars C. Moeller ◽  
Craig Wardrip ◽  
Marek Niekrasz ◽  
Samuel Refetoff ◽  
Roy E. Weiss
Keyword(s):  
Thyroid Hormone ◽  
Human Skin ◽  
Calf Serum ◽  
Skin Fibroblasts ◽  
Hormone Action ◽  
Human Skin Fibroblasts ◽  
Thyroid Hormone Action

In Vitro Cytotoxicity Tests on Cultured Human Skin Fibroblasts to Predict the Irritation Potential of Surfactants

1991 ◽  
Vol 19 (3) ◽  
pp. 324-336
Author(s):  
Marleen Cornells ◽  
Charlotte Dupont ◽  
Jacques Wepierre
Keyword(s):  
Human Skin ◽  
Calf Serum ◽  
In Vitro Cytotoxicity ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Experimental Conditions ◽  
System A ◽  
Brij 35

A comparative study of the cytotoxicity of 17 surfactants was performed in vitro on cultured human skin fibroblasts to predict their irritation potential under different experimental conditions: test media, presence of proteins, various times of exposure (2–72 hours), and evaluation methods. For cytotoxicity, the tetrazolium MTT assay after exposure for 2 hours in Hank's medium (HBSS) seemed to be more sensitive than protein and LDH leakage tests. Cytotoxicities in HBSS and in minimum Eagle's medium (MEM) were very similar. Addition of 10% fetal calf serum (FCS) to MEM decreased the cytotoxicity of surfactants; however, their order of cytotoxicity was generally the same in MEM with or without FCS. Cytotoxicity increased with incubation time, but the overall ranking remained identical. Non-ionic polyoxyethylene 20 ethers (Brij 35, 58, 78 and 99) surfactants, although considered to be non-irritant in vivo, revealed a high cytotoxic effect in our cell culture system. A good correlation with the results of in vivo Draize rabbit eye irritancy was found only when they were excluded.

The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

1984 ◽  
Vol 12 (2) ◽  
pp. 89-97
Author(s):  
Graham R. Elliott ◽  
H.E. Amos ◽  
James W. Bridges
Keyword(s):  
Human Skin ◽  
Psoriasis Patient ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Cell Type ◽  
Normal Human Skin ◽  
Rate Of Growth ◽  
Structural Analogues ◽  
Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

Thyroid hormone excess stimulates the synthesis of proteoglycan in human skin fibroblasts in culture

1990 ◽  
Vol 123 (5) ◽  
pp. 541-549 ◽  
Author(s):  
Yoshimasa Shishiba ◽  
Yasuhiro Takeuchi ◽  
Noriko Yokoi ◽  
Yasunori Ozawa ◽  
Taeko Shimizu
Keyword(s):  
Thyroid Hormone ◽  
Human Skin ◽  
Specific Activity ◽  
Heparan Sulphate ◽  
Skin Fibroblasts ◽  
Proteoglycan Synthesis ◽  
Heparan Sulphate Proteoglycan ◽  
Human Skin Fibroblasts ◽  
Dermatan Sulphate ◽  
Sulphate Proteoglycan

Abstract We previously demonstrated that proteoglycan accumulated in the affected skin of circumscribed pretibial myxedema of Graves' disease. As an underlying mechanism responsible for the accumulation, we sought to determine whether excess thyroid hormone was partially responsible for the increase in proteoglycan synthesis. Human skin fibroblasts were cultured in Ham's F-10 medium containing 1% Nutridoma with graded doses of T3 (0.184 × 10−9 to 46 × 10−9 mol/l) and were labelled with [35S]sulphate and [3H]glucosamine. Proteoglycans were purified by Sephadex G-50, Q-Sepharose chromatography with NaCl-gradient and Sepharose CL-6B chromatography. 35S and 3H incorporated into dermatan sulphate proteoglycan and heparan sulphate proteoglycan and 3H incorporated into hyaluronan were measured. 35S and 3H incorporation into dermatan sulphate proteoglycan was minimum at a T3 concentration of 0.184 × 10−9 mol/l, and increased with increasing doses of T3 up to 46 × 10−9 mol/l. 35S and 3H incorporation into heparan sulphate proteoglycan also increased with increasingdoses of T3. 3H incorporation into hyaluronan was not influenced at all by T3. The increased incorporation of 35S into proteoglycan in high-T3 culture reflects the increased synthesis of proteoglycan because 1. the extent of sulphation of disaccharides examined by thin-layer chromatography was not altered by T3; 2. the specific activity of [35S]sulphate was not influenced by T3, and 3. T3 did not decrease the degradation rate of cell-associated proteoglycan.

scholarly journals Basal Level of Autophagy Is Increased in Aging Human Skin Fibroblasts In Vitro, but Not in Old Skin

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126546 ◽  
Author(s):  
Dino Demirovic ◽  
Carine Nizard ◽  
Suresh I. S. Rattan
Keyword(s):  
Human Skin ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

Selective enrichment and biochemical characterization of seven human skin fibroblasts cell types in vitro

1989 ◽  
Vol 180 (1) ◽  
pp. 84-93 ◽  
Author(s):  
H.Peter Rodemann ◽  
Klaus Bayreuther ◽  
Pal I. Francz ◽  
Klaus Dittmann ◽  
Mario Albiez
Keyword(s):  
Human Skin ◽  
Biochemical Characterization ◽  
Cell Types ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Selective Enrichment
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