Fas Ligand Expression in Thyroid Follicular Cells from Patients with Thionamide-Treated Graves' Disease

Thyroid ◽  
2000 ◽  
Vol 10 (7) ◽  
pp. 527-532 ◽  
Author(s):  
Nicholas Mitsiades ◽  
Vassiliki Poulaki ◽  
Sophia Tseleni-Balafouta ◽  
George P. Chrousos ◽  
Demetrios A. Koutras
Keyword(s):  
Fas Ligand ◽  
Graves Disease ◽  
Follicular Cells ◽  
Thyroid Follicular Cells ◽  
Ligand Expression

scholarly journals Overexpression of Metallothionein I/II: A New Feature of Thyroid Follicular Cells in Graves' Disease

2012 ◽  
Vol 97 (2) ◽  
pp. 446-454 ◽  
Author(s):  
M. Ruiz-Riol ◽  
M. J. Martínez-Arconada ◽  
N. Alonso ◽  
B. Soldevila ◽  
D. Marchena ◽  
...  
Keyword(s):  
Graves Disease ◽  
Follicular Cells ◽  
Thyroid Follicular Cells ◽  
New Feature

scholarly journals Planning of 131I Therapy for Graves Disease Based on the Radiation Dose to Thyroid Follicular Cells

2008 ◽  
Vol 49 (12) ◽  
pp. 2026-2030 ◽  
Author(s):  
D. Eterovic ◽  
Z. Antunovic ◽  
V. Markovic ◽  
D. Grosev
Keyword(s):  
Radiation Dose ◽  
131I Therapy ◽  
Graves Disease ◽  
Follicular Cells ◽  
Thyroid Follicular Cells

Thyroid growth-stimulating immunoglobulins in goitrous disease: relationship to thyroid-stimulating immunoglobulins

1986 ◽  
Vol 111 (3) ◽  
pp. 321-330 ◽  
Author(s):  
P. P. A. Smyth ◽  
N. M. McMullan ◽  
B. Grubeck-Loebenstein ◽  
D. K. O'Donovan
Keyword(s):  
G6pd Activity ◽  
Graves Disease ◽  
Follicular Cells ◽  
Structural Variations ◽  
Thyroid Stimulating Immunoglobulins ◽  
Cytochemical Bioassay ◽  
Thyroid Follicular Cells ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Receptor Antibodies ◽  
Relative Potencies

Abstract. Thyroid growth stimulating immunoglobulins (TGI) were assayed in IgG concentrates prepared from human plasma using a cytochemical bioassay (CBA) based on the measurement of changes in glucose-6-phosphate dehydrogenase (G6PD) activity in guinea pig thyroid follicular cells. TGI was present in all 8 patients studied who had goitrous Graves' disease, in 9 who had toxic diffuse goitres with asymmetric uptake on scintigram and/or symptomatic ophthalmopathy and in 4:8 who had toxic uninodular goitre with autonomously functioning nodules. TGI were also present in 34:54 (64%) of patients who had non-toxic goitres. In contrast, TGI were undetectable in 4 patients who had Graves' disease without palpable goitre and in all 18 euthyroid non-goitrous volunteers. Maximum increases in G6PD activity occurred at an IgG concentration of 50 μg/ml in all patients who had goitrous Graves' disease and in 5:7 who had diffuse non-toxic goitres. In contrast, IgG concentrates from 20:27 patients with nodular goitres caused maximum increases in G6PD activity at an IgG concentration of 500 μg/ml. A comparison of the prevalence of TGI with that of thyroid stimulating immunoglobulins (TSI), also measured by CBA, in 63 patients showed that although both stimulators were present in 8 patients who had goitrous Graves' disease they were only simultaneously present in 18:43 (42%) who had non-toxic goitres of various aetiologies. Thyrotrophin receptor antibodies (TRAb) were present in 11/44 (25%) of non-toxic goitrous patients but there was no significant correlation with IgG stimulators in such patients. It is concluded that the finding of IgG stimulators in a variety of thyroid disorders suggests common immunological features in their pathogenesis. The presence or absence of such stimulators and their relative potencies may play a part in determining functional or structural variations between common forms of goitre.

Expression of an intercellular adhesion molecule, ICAM-1, by human thyroid cells

1989 ◽  
Vol 122 (1) ◽  
pp. 185-NP ◽  
Author(s):  
A. P. Weetman ◽  
S. Cohen ◽  
M. W. Makgoba ◽  
L. K. Borysiewicz
Keyword(s):  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Human Thyroid ◽  
Accessory Cell ◽  
Graves Disease ◽  
Follicular Cells ◽  
Thyroid Cells ◽  
Hla Dr ◽  
Thyroid Follicular Cells

ABSTRACT Intercellular adhesion molecule-1 (ICAM-1), hitherto identified on activated B cells, macrophages, dendritic cells, endothelia and certain epithelial cells, serves as a ligand for the lymphocyte function-associated antigen-1 (LFA-1). ICAM-1 binding by LFA-1 enhances the efficiency of lymphocyte-target cell and lymphocyte-accessory cell interactions. We have investigated the in-vitro expression of ICAM-1 by cultured thyroid cells from five patients with Graves' disease using indirect immunofluorescence analysis, and found that 30 ± 11% (mean ± s.d.) of cells were ICAM-1 positive under basal conditions. The proportion of cells which were ICAM-1 positive and the amount of ICAM-1 per cell (assessed by fluorescence intensity) were both increased in all cases by the cytokines γ-interferon, interleukin-1 and tumour necrosis factor. Immunohistochemical analysis of frozen sections from thyroidectomy specimens demonstrated ICAM-1 on thyroid follicular cells in areas of lymphocytic infiltration in patients with Graves' disease (n = 2) or Hashimoto's thyroiditis (n = 2). ICAM-1 was not found in specimens from a patient with a toxic multinodular goitre or a patient with Graves' disease without focal lymphocytic accumulation. These results suggest that the thyroid epithelium may express ICAM-1 as well as major histocompatibility complex class II antigens, such as HLA-DR, in response to locally synthesized cytokines. The enhanced expression of ICAM-1 may render these cells more susceptible as targets for lymphocytemediated cytotoxicity, and together with HLA-DR antigen expression may increase the accessory cell capability of the thyroid follicular cells. Journal of Endocrinology (1989) 122, 185–191

Activated interstitial and intraepithelial thyroid lymphocytes in autoimmune thyroid disease

1988 ◽  
Vol 119 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Shara B. Cohen ◽  
Anthony P. Weetman
Keyword(s):  
T Cells ◽  
Autoimmune Thyroiditis ◽  
Transferrin Receptor ◽  
Autoimmune Thyroid Disease ◽  
Receptor Expression ◽  
Graves Disease ◽  
Follicular Cells ◽  
Autoimmune Thyroid ◽  
Thyroid Follicular Cells ◽  
Transferrin Receptor Expression

Abstract. This study has further characterised the thyroid lymphocytic infiltrate in Graves' disease and Hashimoto's thyroiditis. Two population of lymphocytes were identified. The interstitial population occurred as a diffuse and a focal infiltrate; most cells were CD3-positive (T cells) and in 4 of 6 glands CD8 (suppressor-cytotoxic)-positive T cells predominated. The intraepithelial population was CD3-negative, CD8-positive. Both populations also contained a few NK (Leu 11b positive cells) in some glands. Many of the lymphocytes in both populations stained with UCHL1 and RFT2 suggesting that these are primed and activated cells, borne out by staining for transferrin receptor expression. Although thyroid follicular cells were Ia-positive, macrophages and dentritic cells were found in all cases, so that a role for antigen-presentation by all three potential candidates in autoimmune thyroiditis is possible.

Expression of nitric oxide synthase III in human thyroid follicular cells: evidence for increased expression in hyperthyroidism

1997 ◽  
Vol 136 (6) ◽  
pp. 649-655 ◽  
Author(s):  
Ides M Colin ◽  
Peter Kopp ◽  
Jakob Zbären ◽  
André Häberli ◽  
William E Grizzle ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Thyroid Gland ◽  
Tsh Receptor ◽  
Human Thyroid ◽  
Graves Disease ◽  
Follicular Cells ◽  
Thyroid Follicular Cells ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

Abstract Nitric oxide mediates a wide array of cellular functions in many tissues. It is generated by three known isoforms of nitric oxide synthases (NOS). Recently, the endothelial isoform, NOSIII, was shown to be abundantly expressed in the rat thyroid gland and its expression increased in goitrous glands. In this study, we analyzed whether NOSIII is expressed in human thyroid tissue and whether levels of expression vary in different states of thyroid gland function. Semiquantitative RT-PCR was used to assess variations in NOSIII gene expression in seven patients with Graves' disease, one with a TSH-receptor germline mutation and six hypothyroid patients (Hashimoto's thyroiditis). Protein expression and subcellular localization were determined by immunohistochemistry (two normal thyroids, five multinodular goiters, ten hyperthyroid patients and two hypothyroid patients). NOSIII mRNA was detected in all samples: the levels were significantly higher in tissues from hyperthyroid patients compared with euthyroid and hypothyroid patients. NOSIII immunoreactivity was detected in vascular endothelial cells, but was also found in thyroid follicular cells. In patients with Graves' disease, the immunostaining was diffusely enhanced in all follicular cells. A more intense signal was observed in toxic adenomas and in samples obtained from a patient with severe hyperthyroidism due to an activating mutation in the TSH receptor. In multinodular goiters, large follicles displayed a weak signal whereas small proliferative follicles showed intense immunoreactivity near the apical plasma membrane. In hypothyroid patients, NOSIII immunoreactivity was barely detectable. In summary, NOSIII is expressed both in endothelial cells and thyroid follicular cells. The endothelial localization of NOSIII is consistent with a role for nitric oxide in the vascular control of the thyroid. NOSIII expression in thyroid follicular cells and the variations in its immunoreactivity suggest a possible role for nitric oxide in thyrocyte function and/or growth. European Journal of Endocrinology 136 649–655

scholarly journals Transgenic Expression of Fas Ligand on Thyroid Follicular Cells Prevents Autoimmune Thyroiditis

2000 ◽  
Vol 164 (4) ◽  
pp. 1681-1688 ◽  
Author(s):  
Frédéric Batteux ◽  
Patrick Lores ◽  
Danièle Bucchini ◽  
Gilles Chiocchia
Keyword(s):  
Autoimmune Thyroiditis ◽  
Fas Ligand ◽  
Follicular Cells ◽  
Transgenic Expression ◽  
Thyroid Follicular Cells

Adherence of Rfd-1 Positive Dendritic Cells to the Basal Surface of Thyroid Follicular Cells in Graves' Disease

Autoimmunity ◽  
1994 ◽  
Vol 17 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Johan Molne ◽  
Svante Jansson ◽  
Larse Ericson ◽  
Mikael Nilsson
Keyword(s):  
Dendritic Cells ◽  
Graves Disease ◽  
Follicular Cells ◽  
Basal Surface ◽  
Thyroid Follicular Cells

Antithyroid Drugs Inhibit In Vivo HLA-DR Expression in Thyroid Follicular Cells in Graves' Disease

Thyroid ◽  
2001 ◽  
Vol 11 (6) ◽  
pp. 575-580 ◽  
Author(s):  
Denise Engelbrecht Zantut-Wittmann ◽  
Marcos Antonio Tambascia ◽  
Miriam Aparecida da Silva Trevisan ◽  
Glauce Aparecida Pinto ◽  
José Vassallo
Keyword(s):  
Antithyroid Drugs ◽  
Graves Disease ◽  
Follicular Cells ◽  
Hla Dr ◽  
Thyroid Follicular Cells
Sign in / Sign up

Export Citation Format

Share Document