Prevalence and Significance of Antibodies Reactive with Eye Muscle Membrane Antigens in Sera from Patients with Graves' Ophthalmopathy and Other Thyroid and Nonthyroid Diseases

Thyroid ◽  
1998 ◽  
Vol 8 (2) ◽  
pp. 167-174 ◽  
Author(s):  
YI-JIE WU ◽  
SUSAN E.M. CLARKE ◽  
PHILIP SHEPHERD
Keyword(s):  
Muscle Membrane ◽  
Eye Muscle ◽  
Graves Ophthalmopathy ◽  
Membrane Antigens

Evaluation of an enzyme-linked immunosorbent assay for the measurement of autoantibodies against eye muscle membrane antigens in Graves' ophthalmopathy

1986 ◽  
Vol 113 (4) ◽  
pp. 514-522 ◽  
Author(s):  
A. Miller ◽  
H. Sikorska ◽  
M. Salvi ◽  
J. R. Wall
Keyword(s):  
Immunosorbent Assay ◽  
Specific Binding ◽  
Normal Subjects ◽  
Muscle Membrane ◽  
Eye Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eye Muscle ◽  
Isolation And Purification ◽  
Graves Ophthalmopathy ◽  
Membrane Antigens

Abstract. We have tested for antibodies against human and pig eye muscle membrane antigens in the serum of patients with Graves' ophthalmopathy using an enzyme-linked immunosorbent assay (ELISA). Several different membrane preparations were used as source of putative antigen including a 100 000 × g pellet, a pellet depleted of the 100 000 × g (microsome) fraction, and solubilized membranes. With eye muscle membrane pellets there were no significant differences for either serum or immunoglobulins between patients with ophthalmopathy, those with autoimmune thyroid disorders without eye disease, and normal subjects for either human or pig membranes, although tests were positive determined from the upper limit of normal in a few patients with or without eye disease. This was the case regardless of the enzyme-antibody conjugate used, the membrane protein concentration or serum or immunoglobulin dilution. Pre-absorption of tissue fractions, serum, or immunoglobulins, with red blood cells or liver powder, eye muscle membranes or skeletal muscle membranes did not significantly reduce background binding which was often very high, or enhance the difference between patients with ophthalmopathy and normal subjects. It was found that non-specific binding to the plastic surface of the microplates and/or tissue proteins, the presence, in human tissues, of blood-derived immunoglobulins which gave strong reactions in the ELISA, and variable fixation of membrane pellets to the plates were factors which made ELISA unsatisfactory when crude membrane pellets were used as antigen. When eye muscle membranes solubilized with standard agents including SDS, Triton X-100 and deoxycholine were tested, again no differences were demonstrated between patients with Graves' ophthalmopathy and normal subjects. However, when membranes were solubilized with the zwitterionic agent 'CHAPSO' approximately 20% of patients with Graves' ophthalmopathy and smaller proportions of patients with thyroid disease without apparent eye involvement had positive tests with both human and pig eye muscle. While availability of human monoclonal antibodies should soon allow the isolation and purification of soluble eye muscle membrane autoantigens for use in sensitive and specific antibody tests, the ELISA, as presently used with crude tissue fractions, appears too variable and prone to non-specific immunoglobulin-binding for routine clinical use.

Re-evaluation of eye muscle autoantibody determination in Graves' ophthalmopathy: failure to detect a specific antigen by use of enzyme-linked immunosorbent assay, indirect immunofluorescence, and immunoblotting techniques

1989 ◽  
Vol 121 (5) ◽  
pp. 643-650 ◽  
Author(s):  
E. Schifferdecker ◽  
U. Ketzler-Sasse ◽  
B. O. Boehm ◽  
H. B. Ronsheimer ◽  
W. A. Scherbaum ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Indirect Immunofluorescence ◽  
Specific Antigen ◽  
Antigenic Structure ◽  
Muscle Membrane ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eye Muscle ◽  
Human Eye ◽  
Graves Ophthalmopathy ◽  
Indirect Immunofluorescence Technique

Abstract. Sera from 41 patients with Graves' ophthalmopathy were investigated for presence of autoantibodies directed against eye muscle preparations using different methods: 1. ELISA with pork eye muscle membrane preparations; 2. Immunoblotting with glycoprotein preparations from human eye muscle; 3. Indirect immunofluorescence with human eye muscle sections. The ELISA was not suitable for detection of specific immunoglobulin binding with sera from patients suffering from endocrine ophthalmopathy. Immunoblotting exhibited only nonspecific binding to some muscle proteins; it could be prevented by pre-adsorption procedures and was not different from the pattern observed with skeletal muscle as antigen. The indirect immunofluorescence technique revealed no binding of Graves' sera to human muscle sections, whereas sera containing antibodies against skeletal muscle bound to eye muscle as well. Thus far, an antigenic structure of eye muscle specific for Graves' ophthalmopathy is not detectable with the methodology used here. The possibility that retroorbital connective tissue may be the main target of the autoimmune process must be considered.

Serum Antibodies Reactive with Eye Muscle Membrane Antigens Are Detected in Patients with Nonspecific Orbital Inflammation

Ophthalmology ◽  
1995 ◽  
Vol 102 (1) ◽  
pp. 145-153 ◽  
Author(s):  
Cagla Atabay ◽  
Anna Tyutyunikov ◽  
Deborah Scalise ◽  
Carol Stolarski ◽  
Melvin B. Hayes ◽  
...  
Keyword(s):  
Muscle Membrane ◽  
Serum Antibodies ◽  
Eye Muscle ◽  
Orbital Inflammation ◽  
Membrane Antigens

Serum Antibodies Reactive with Eye Muscle Membrane Antigens Are Detected in Patients with Nonspecific Orbital Inflammation

1996 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
C Atabay ◽  
A Tyutyunikov ◽  
D Scalise ◽  
C Stolarski ◽  
M B Hayes ◽  
...  
Keyword(s):  
Muscle Membrane ◽  
Serum Antibodies ◽  
Eye Muscle ◽  
Orbital Inflammation ◽  
Membrane Antigens

Muscle and species reactivity of mouse monoclonal antibodies to human eye muscle membrane antigens

1991 ◽  
Vol 59 (1) ◽  
pp. 104-116 ◽  
Author(s):  
B. Teboul ◽  
H. Triller ◽  
F. Chung ◽  
N. Bernard ◽  
Z.-G. Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Muscle Membrane ◽  
Eye Muscle ◽  
Human Eye ◽  
Membrane Antigens

Detection and immunomodulation of eye muscle antibodies in Graves' ophthalmopathy

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S12
Author(s):  
G. KAHALY ◽  
R. MONCAYO ◽  
U. BEMETZ ◽  
U. KRAUSE ◽  
J. BEYER
Keyword(s):  
Eye Muscle ◽  
Graves Ophthalmopathy
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