Localization and Clinical Significance of Thyrotropin Receptor mRNA Expression in Orbital Fat and Eye Muscle Tissues from Patients with Thyroid-Associated Ophthalmopathy

Thyroid ◽  
1996 ◽  
Vol 6 (6) ◽  
pp. 553-562 ◽  
Author(s):  
YUJI HIROMATSU ◽  
MASAYUKI SATO ◽  
YOICHI INOUE ◽  
MARI KOGA ◽  
IKUYO MIYAKE ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Clinical Significance ◽  
Thyrotropin Receptor ◽  
Eye Muscle ◽  
Orbital Fat ◽  
Receptor Mrna ◽  
Muscle Tissues ◽  
Thyroid Associated Ophthalmopathy

scholarly journals Thyrotropin Receptor Transcripts in Human Adipose Tissue

1997 ◽  
Vol 82 (6) ◽  
pp. 2003-2005 ◽  
Author(s):  
Ms Crisp ◽  
C Lane ◽  
M Halliwell ◽  
D Wynford-Thomas ◽  
M Ludgate
Keyword(s):  
Adipose Tissue ◽  
Limit Of Detection ◽  
Pcr Amplification ◽  
Human Adipose Tissue ◽  
Target Antigen ◽  
Thyrotropin Receptor ◽  
Orbital Fat ◽  
Muscle Tissues ◽  
Thyroid Associated Ophthalmopathy ◽  
Autoimmune Pathogenesis

Abstract Thyroid associated ophthalmopathy (TAO) is generally considered to have an autoimmune pathogenesis but the target antigen has yet to be identified. It is most frequently associated with Graves’ disease and there is some logic in assuming that the same antigen, the thyrotropin receptor (TSHR), is the common link. Previous studies, mostly PCR based, aimed at investigating TSHR transcripts in the orbit, have yielded conflicting results, although there is circumstantial evidence for their presence in orbital fat. In this study, we have examined adult human adipose and muscle tissues from various locations, initially by PCR and subsequently by northern blot. We obtained the expected 610bp product in normal intestinal and orbital fat but not skeletal muscle, following two rounds of PCR amplification but only when reverse transcription used a TSHR specific primer. In northern blots, despite loading all of the RNA obtained from total normal orbital fat contents, TSHR transcripts were at the limit of detection and similarly for large samples of intestinal fat. The exception was RNA obtained from TAO orbital fat, in which TSHR transcripts of 4.6 and 1.7kb were clearly visible, as in the thyroid. We conclude that normal adult adipose tissues contain low levels of TSHR transcripts. In TAO, TSHR transcripts are elevated probably due to an increased number of cells, in particular of preadipocytes in orbital adipose tissue.

scholarly journals Cytokine Profiles in Eye Muscle Tissue and Orbital Fat Tissue from Patients with Thyroid-Associated Ophthalmopathy*

2000 ◽  
Vol 85 (3) ◽  
pp. 1194-1199 ◽  
Author(s):  
Yuji Hiromatsu ◽  
Dame Yang ◽  
Tomasz Bednarczuk ◽  
Ikuyo Miyake ◽  
Kyohei Nonaka ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Fat Tissue ◽  
Eye Muscle ◽  
Orbital Fat ◽  
Cytokine Profiles ◽  
Thyroid Associated Ophthalmopathy

PTH-related protein and Type 1 PTH receptor mRNA expression in ventricular myocardial hypertrophy

Therapy ◽  
2005 ◽  
Vol 2 (3) ◽  
pp. 415-423
Author(s):  
Antony Halapas ◽  
Peter Lembessis ◽  
Antigone Sourla ◽  
Costas Pantos ◽  
Dennis V Cokkinos ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Myocardial Hypertrophy ◽  
Related Protein ◽  
Receptor Mrna

Long-Term Use of GLP-1 Receptor Agonists Alter GLP-1 Receptor mRNA Expression in Hindbrain Pathways That Regulate Gastric Motility in Mice

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1088-P
Author(s):  
PEILI WANG ◽  
RAMANAIAH MAMILLAPALLI ◽  
WANLING ZHU ◽  
OWEN CHAN
Keyword(s):  
Mrna Expression ◽  
Gastric Motility ◽  
Receptor Agonists ◽  
Receptor Mrna
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