Intercellular Adhesion Molecule-1 (ICAM-1) in the Sera of Patients with Graves' Disease: Correlation with Disease Activity and Treatment Status

Thyroid ◽  
1995 ◽  
Vol 5 (5) ◽  
pp. 373-377 ◽  
Author(s):  
HIROSHI FUKAZAWA ◽  
KATSUMI YOSHIDA ◽  
NOBUKO RAISE ◽  
YOSHINORI KISO ◽  
NORIYO SAYAMA ◽  
...  
Keyword(s):  
Disease Activity ◽  
Adhesion Molecule ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Graves Disease ◽  
Intercellular Adhesion Molecule 1 ◽  
Treatment Status

Expression of membrane and soluble intercellular adhesion molecule-1 in Graves' disease

1997 ◽  
Vol 19 (2) ◽  
pp. 191-201 ◽  
Author(s):  
C Massart ◽  
E Sonnet ◽  
J Gibassier ◽  
N Genetet ◽  
G Leclech ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Adhesion Molecule ◽  
Calf Serum ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Human Thyroid ◽  
Graves Disease ◽  
Intercellular Adhesion Molecule 1 ◽  
Thyroid Cells ◽  
Soluble Intercellular Adhesion Molecule

We have investigated the in vitro expression of membrane and soluble intercellular adhesion molecule-1 (ICAM-1) by human thyroid cells from 20 patients with Graves' disease and 5 normal subjects. Membrane ICAM-1 was not detected by flow cytometry analysis in non-cultured thyrocytes from either normal or Graves' disease tissues. It appeared on thyroid cells after a 24-h culture in monolayers and showed a regular dose-dependent increase. The same results were obtained with soluble ICAM-1 (sICAM-1) in culture media from cells cultured in monolayers, vesicles or follicles. No change was obtained with different concentrations of fetal calf serum added to the media. Coculture of Graves' disease thyrocytes with autologous peripheral blood lymphocytes (PBL) or intrathyroidal lymphocytes (ITL) enhanced the expression of both membrane and sICAM-1 whatever the culture model. When normal thyrocytes were cocultured with PBL, sICAM-1 increased but with ITL sICAM-1 remained unchanged. High concentrations of gamma interferon induced an increase of both membrane and sICAM-1 in the three culture models. However the increases were greater with vesicles and follicles. Only sICAM-1 levels were raised with 0.1, 1 and 10 microM retinoic acid. These results suggest that ICAM-1 appears in culture, possibly due to mechanical effects such as adherence to plates and cell-to-cell contacts. Moreover, its expression is modulated by several factors such as cytokines or retinoic acid. Further investigations are needed to establish whether ICAM-1 is really involved in the pathogenesis of Graves' disease.

A Circadian Variation Exists for Soluble Levels of Intercellular Adhesion Molecule-I and E-Selectin in Healthy Volunteers

1998 ◽  
Vol 94 (5) ◽  
pp. 537-540 ◽  
Author(s):  
C. Maple ◽  
G. Kirk ◽  
M. McLaren ◽  
D. Veale ◽  
J.J.F. Belch
Keyword(s):  
Cell Adhesion ◽  
Disease Activity ◽  
Diurnal Variation ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecules ◽  
Circadian Variation ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Soluble Intercellular Adhesion Molecule

1. We have previously shown a circadian variation in leucocyte activation and endothelial function which may explain why some inflammatory and vascular diseases show a circadian variation in disease activity/occurrence. 2. We have investigated the circadian variation of two soluble cell adhesion molecules, intercellular adhesion molecule-1 and E-selectin, in 10 healthy volunteers. Soluble intercellular adhesion molecule-1 is released from both activated leucocytes and endothelial cells while soluble E-selectin is released only from activated endothelium. 3. Results show a circadian variation exists for both soluble intercellular adhesion molecule-1 and E-selectin (both P < 0.0001, analysis of variance) with a peak activity at 12:00 h for both measures and a minimum activity at 04:00 h for intercellular adhesion molecule-1 and 00:00 h for E-selectin. 4. These results demonstrate the existence of a diurnal variation in cell adhesion molecules, providing evidence in support of a diurnal pattern in endothelial and leucocyte activation. An alteration in this biological rhythm may help to explain the diurnal variation in disease activity in certain inflammatory and vascular disease states. Furthermore, it stresses the importance of sample time point standardization in clinical studies.

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