Three-Dimensional Cell Sheet Construction Method with a Polyester Micromesh Sheet

2020 ◽  
Vol 26 (3) ◽  
pp. 170-179
Author(s):  
Takeshi Hori ◽  
Osamu Kurosawa ◽  
Kohei Ishihara ◽  
Taro Mizuta ◽  
Hiroo Iwata
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
Construction Method ◽  
Dimensional Cell

scholarly journals Pulsatile Cardiac Tissue Grafts Using a Novel Three-Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, In Vivo

2006 ◽  
Vol 98 (5) ◽  
pp. 705-712 ◽  
Author(s):  
Akira Furuta ◽  
Shunichiro Miyoshi ◽  
Yuji Itabashi ◽  
Tatsuya Shimizu ◽  
Shinichiro Kira ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Tissue Grafts ◽  
Dimensional Cell

scholarly journals In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair

2018 ◽  
Vol 19 (8) ◽  
pp. 2272 ◽  
Author(s):  
Chi-Fen Hsieh ◽  
Zexing Yan ◽  
Ricarda Schumann ◽  
Stefan Milz ◽  
Christian Pfeifer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Sheet ◽  
Tendon Repair ◽  
Three Dimensional ◽  
Cell Types ◽  
Tendon Tissue ◽  
Cell Sheets ◽  
Tendon Tissue Engineering ◽  
Dimensional Cell

The poor and slow healing capacity of tendons requires novel strategies to speed up the tendon repair process. Hence, new and promising developments in tendon tissue engineering have become increasingly relevant. Previously, we have established a tendon progenitor cell line via ectopic expression of the tendon-related basic helix-loop-helix (bHLH) transcription factor Scleraxis (Scx) in human bone marrow mesenchymal stem cells (hMSC-Scx). The aim of this study was to directly compare the characteristics of hMSC-Scx cells to that of primary human tendon stem/progenitors cells (hTSPCs) via assessment of self-renewal and multipotency, gene marker expression profiling, in vitro wound healing assay and three-dimensional cell sheet formation. As expected, hTSPCs were more naive than hMSC-Scx cells because of higher clonogenicity, trilineage differentiation potential, and expression of stem cell markers, as well as higher mRNA levels of several gene factors associated with early tendon development. Interestingly, with regards to wound healing, both cell types demonstrate a comparable speed of scratch closure, as well as migratory velocity and distance in various migration experiments. In the three-dimensional cell sheet model, hMSC-Scx cells and hTSPCs form compact tendinous sheets as histological staining, and transmission electron microscopy shows spindle-shaped cells and collagen type I fibrils with similar average diameter size and distribution. Taken together, hTSPCs exceed hMSC-Scx cells in several characteristics, namely clonogenicity, multipotentiality, gene expression profile and rates of tendon-like sheet formation, whilst in three-dimensional cell sheets, both cell types have comparable in vitro healing potential and collagenous composition of their three-dimensional cell sheets, making both cell types a suitable cell source for tendon tissue engineering and healing.

scholarly journals Noninvasive cross-sectional observation of three-dimensional cell sheet-tissue-fabrication by optical coherence tomography

2015 ◽  
Vol 2 ◽  
pp. 57-62 ◽  
Author(s):  
Yuji Haraguchi ◽  
Tatsuya Shimizu ◽  
Kiminori Mizuuchi ◽  
Hiroto Kawata ◽  
Mari Kobayashi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Dimensional Cell

Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology

Biomaterials ◽  
2010 ◽  
Vol 31 (7) ◽  
pp. 1646-1654 ◽  
Author(s):  
Tadashi Sasagawa ◽  
Tatsuya Shimizu ◽  
Sachiko Sekiya ◽  
Yuji Haraguchi ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Cell Sheet ◽  
Three Dimensional ◽  
A Cell ◽  
Dimensional Cell

Dentin-pulp complex tissue regeneration via three-dimensional cell sheet layering

2021 ◽  
Author(s):  
YAN HUIJIAO ◽  
Masamitsu Oshima ◽  
Resmi Raju ◽  
Swarna Raman ◽  
Kazumitsu Sekine ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

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