scholarly journals In Vitro Evaluation of Recombinant Bone Morphogenetic Protein-2 Bioactivity for Regenerative Medicine

2019 ◽  
Vol 25 (9) ◽  
pp. 553-559
Author(s):  
Stephanie L. Fung ◽  
Xiaohuan Wu ◽  
Julian P. Maceren ◽  
Yong Mao ◽  
Joachim Kohn
Keyword(s):  
Regenerative Medicine ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
In Vitro Evaluation ◽  
Morphogenetic Protein

NEW RECOMBINANT BONE MORPHOGENETIC PROTEIN-2 (BMP-2): OSTEOGENIC ACTIVITY IN THE COMPOSITION WITH VARIOUS MATERIALS FOR REGENERATIVE MEDICINE

2020 ◽  
pp. 71-72
Author(s):  
A. Gromov ◽  
A. Karyagina
Keyword(s):  
Regenerative Medicine ◽  
High Activity ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Osteogenic Activity ◽  
Synthetic Materials ◽  
Morphogenetic Protein ◽  
New Variant ◽  
Osteogenesis In Vitro

A new variant of recombinant prokaryotic bone morphogenetic protein-2 (rhBMP-2) is characterized by high activity in vitro and induces pronounced osteogenesis in vitro in the composition with various natural and synthetic materials.

The Effect of Bone Morphogenetic Protein-2 on Rat Intervertebral Disc Cells in Vitro

Spine ◽  
2003 ◽  
Vol 28 (16) ◽  
pp. 1773-1780 ◽  
Author(s):  
S. Tim Yoon ◽  
Keun Su Kim ◽  
Jun Li ◽  
Jin Soo Park ◽  
Tomoyuki Akamaru ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Disc Cells

Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression

2021 ◽  
Author(s):  
Meng Wang ◽  
Hong Sung Min ◽  
Haojie Shan ◽  
Yiwei Lin ◽  
Wenyang Xia ◽  
...  
Keyword(s):  
Femoral Head ◽  
Bone Morphogenetic Protein ◽  
Inflammatory Responses ◽  
Gene Knockout ◽  
Osteoclast Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Qrt Pcr ◽  
Morphogenetic Protein ◽  
Local Expression

Increased inflammatory responses is one of the major characteristics of osteonecrosis of the femoral head (ONFH). We aimed to investigate the function of bone morphogenetic protein 2 (BMP-2)/interleukin (IL)-34 axis in the inflammatory responses of ONFH. The systemic and local expression of BMPs in ONFH patients were detected by qRT-PCR and ELISA. In vitro osteoclast differentiation and ONFH mouse models, induced by 20 mg/kg methylprednisolone through intramuscular injection, were established using wild type and BMP-2-/- mice to explore the regulatory role of BMP-2 in pro-inflammatory responses and bone defects of ONFH. IL-34 expression and function were examined in vitro and in vivo through qRT-PCR, TRAP staining, and gene knockout. The systemic and local expression of BMPs were elevated in ONFH patients. BMP-2 reduced the production of pro-inflammatory cytokines and inhibited the differentiation of osteoclasts. Mechanistically, BMP-2 inhibited osteoclasts formation through suppressing IL-34 expression, and then promoted bone repair and alleviated ONFH. In conclusion, our study reveals that BMP-2 inhibits inflammatory responses and osteoclast formation through down-regulating IL-34.

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