scholarly journals Optimization and Characterization of Calcium Phosphate Transfection in Mesenchymal Stem Cells

2019 ◽  
Vol 25 (9) ◽  
pp. 543-552 ◽  
Author(s):  
Chi-Wen Lo ◽  
Tzuhua Lin ◽  
Masaya Ueno ◽  
Monica Romero-Lopez ◽  
Masahiro Maruyama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Calcium Phosphate Transfection

Isolation and characterization of exosomes from adipose tissue‐derived mesenchymal stem cells

2020 ◽  
Author(s):  
Elsa González‐Cubero ◽  
María Luisa González‐Fernández ◽  
Laura Gutiérrez‐Velasco ◽  
Eliezer Navarro‐Ramírez ◽  
Vega Villar‐Suárez
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal stem cells in Xenopus laevis

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Xenopus Laevis ◽  
Isolation And Characterization

Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

2009 ◽  
Vol 132 (5) ◽  
pp. 533-546 ◽  
Author(s):  
Erdal Karaoz ◽  
Ayça Aksoy ◽  
Selda Ayhan ◽  
Ayla Eker Sarıboyacı ◽  
Figen Kaymaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Rat Bone

Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

2009 ◽  
Vol 5 (4) ◽  
pp. 1294-1305 ◽  
Author(s):  
L. Saldaña ◽  
S. Sánchez-Salcedo ◽  
I. Izquierdo-Barba ◽  
F. Bensiamar ◽  
L. Munuera ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Human Mesenchymal Stem Cells

scholarly journals Attachment, Proliferation, and Chondroinduction of Mesenchymal Stem Cells on Porous Chitosan-Calcium Phosphate Scaffolds

2013 ◽  
Vol 7 (1) ◽  
pp. 275-281 ◽  
Author(s):  
Steven Elder ◽  
Anuhya Gottipati ◽  
Hilary Zelenka ◽  
Joel Bumgardner
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Treatment Options ◽  
Preliminary Investigation ◽  
Osteochondral Lesions ◽  
Porous Chitosan ◽  
Tissue Engineering Approach ◽  
Osteochondral Regeneration ◽  
Further Development

Symptomatic osteochondral lesions occur frequently, but relatively few treatment options are currently available. The purpose of this study was to conduct a preliminary investigation into a new tissue engineering approach to osteochondral regeneration. The concept is a biphasic construct consisting of a porous, osteoconductive chitosan-calcium phosphate scaffold supporting a layer of neocartilage formed by marrow-derived mesenchymal stem cells. Two experiments were conducted to assess the feasibility of this approach. The first experiment characterized the attachment efficiency and proliferation of primary human marrow-derived mesenchymal stem cells seeded relatively sparely onto the scaffold’s surface. The second experiment compared two different methods of creating a biphasic construct using a much higher density of primary porcine marrow stromal cells. About 40% of the sparsely seeded human cells attached and proliferated rapidly. Constructs formed by one of the two experimental techniques exhibited a layer of cartilaginous tissue which only partially covered the scaffold’s surface due to inadequate adhesion between the cells and the scaffold. This study demonstrates some potential for the approach to yield an implantable biphasic construct, but further development is required to improve cell-scaffold adhesion.

Development and characterization of Gal KO porcine bone marrow‐derived mesenchymal stem cells

2021 ◽  
Author(s):  
Takeshi Kikuchi ◽  
Masuhiro Nishimura ◽  
Maki Hirata ◽  
Fuminori Tanihara ◽  
Natsuki Komori ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells
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