Human Amniotic Membrane with Aligned Electrospun Fiber as Scaffold for Aligned Tissue Regeneration

2018 ◽  
Vol 24 (6) ◽  
pp. 368-378 ◽  
Author(s):  
Hanis Hasmad ◽  
Mohd Reusmaazran Yusof ◽  
Zainul Rashid Mohd Razi ◽  
Ruszymah Bt Hj Idrus ◽  
Shiplu Roy Chowdhury
Keyword(s):  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Electrospun Fiber ◽  
Human Amniotic Membrane

scholarly journals Sub-Regional Differences of the Human Amniotic Membrane and Their Potential Impact on Tissue Regeneration Application

2021 ◽  
Vol 8 ◽  
Author(s):  
Adelheid Weidinger ◽  
Laura Poženel ◽  
Susanne Wolbank ◽  
Asmita Banerjee
Keyword(s):  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Anatomical Location ◽  
Differentiation Potential ◽  
Proliferation And Differentiation ◽  
Reprogramming Efficiency ◽  
Potential Impact ◽  
Structural Entity ◽  
Fine Tune

For more than 100 years, the human amniotic membrane (hAM) has been used in multiple tissue regeneration applications. The hAM consists of cells with stem cell characteristics and a rich layer of extracellular matrix. Undoubtedly, the hAM with viable cells has remarkable properties such as the differentiation potential into all three germ layers, immuno-modulatory, and anti-fibrotic properties. At first sight, the hAM seems to be one structural entity. However, by integrating its anatomical location, the hAM can be divided into placental, reflected, and umbilical amniotic membrane. Recent studies show that cells of these amniotic sub-regions differ considerably in their properties such as morphology, structure, and content/release of certain bioactive factors. The aim of this review is to summarize these findings and discuss the relevance of these different properties for tissue regeneration. In summary, reflected amnion seems to be more immuno-modulatory and could have a higher reprogramming efficiency, whereas placental amnion seems to be pro-inflammatory, pro-angiogenic, with higher proliferation and differentiation capacity (e.g., chondrogenic and osteogenic), and could be more suitable for certain graft constructions. Therefore, we suggest that the respective hAM sub-region should be selected in consideration of its desired outcome. This will help to optimize and fine-tune the clinical application of the hAM.

scholarly journals Human amniotic membrane grafts for retinal breaks in diabetic tractional retinal detachment and combined tractional and rhegmatogenous retinal detachment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yen-Chih Chen ◽  
San-Ni Chen
Keyword(s):  
Retinal Detachment ◽  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Rhegmatogenous Retinal Detachment ◽  
Case Series ◽  
Control Group ◽  
Human Amniotic Membrane ◽  
Ellipsoid Zone ◽  
Retinal Breaks ◽  
Additional Surgery

AbstractTo describe the surgical outcomes of using human amniotic membrane (hAM) grafts in the management of retinal breaks in diabetic tractional detachment (TRD) and combined tractional and rhegmatogenous retinal detachment (CTRRD). A retrospective case series of 10 eyes with TRD or CTRRD receiving pars plana vitrectomy with hAM grafts implantation, compared with 13 controls receiving the same surgery without hAM grafts. Best-corrected visual acuity (BCVA) and re-detachment rate were compared between two groups. Postoperatively, all eyes in the hAM group had retina attachment without recurrence, while 9 eyes in the control group had retina re-detachment and required additional surgery (0% vs 69.2%, p = 0.003). The BCVA significantly improved in the hAM group (from 1.96 ± 0.95 to 1.44 ± 0.77 in log MAR, p = 0.03), but not improved in control group (p = 0.20). Postoperative optical coherence tomography of the eyes receiving hAM grafts demonstrated glial tissue regeneration and restoration of ellipsoid zone. In diabetic TRD or CTRRD, hAM grafts could be an effective method, with promising outcome. Compared to standard surgery, it could result in higher retina reattachment rate and significant visual improvement. Moreover, it may offer the adjunctive benefit in tissue regeneration and fasten ellipsoid zone restoration.

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis

2015 ◽  
Vol 57 (5) ◽  
pp. 35-41
Author(s):  
K. Sereda ◽  
◽  
G. Drozhzhina ◽  
T. Gaidamaka ◽  
V. Vit ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Bacterial Keratitis

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Life Sciences ◽  
2021 ◽  
pp. 119157
Author(s):  
Ping Chen ◽  
Minjun Lu ◽  
Tao Wang ◽  
Dongchun Dian ◽  
Yong Zhong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Delivery Vehicle

scholarly journals The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

Biomedicines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Taja Železnik Ramuta ◽  
Larisa Tratnjek ◽  
Aleksandar Janev ◽  
Katja Seme ◽  
Marjanca Starčič Erjavec ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Amniotic Membrane ◽  
Multidrug Resistant ◽  
Basic Knowledge ◽  
Resistant Bacteria ◽  
Human Amniotic Membrane ◽  
Multidrug Resistant Bacteria ◽  
Tract Infections

Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria.

scholarly journals A Novel Phenotype of Junctional Epidermolysis Bullosa with Transient Skin Fragility and Predominant Ocular Involvement Responsive to Human Amniotic Membrane Eyedrops

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 716
Author(s):  
Daniele Castiglia ◽  
Paola Fortugno ◽  
Angelo Giuseppe Condorelli ◽  
Sabina Barresi ◽  
Naomi De Luca ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Epidermolysis Bullosa ◽  
Ocular Involvement ◽  
Human Amniotic Membrane ◽  
Vitro Assay ◽  
Junctional Epidermolysis Bullosa ◽  
Mucosal Involvement ◽  
Skin Fragility ◽  
Laminin 332

Junctional epidermolysis bullosa (JEB) is a clinically and genetically heterogeneous skin fragility disorder frequently caused by mutations in genes encoding the epithelial laminin isoform, laminin-332. JEB patients also present mucosal involvement, including painful corneal lesions. Recurrent corneal abrasions may lead to corneal opacities and visual impairment. Current treatments are merely supportive. We report a novel JEB phenotype distinguished by the complete resolution of skin fragility in infancy and persistent ocular involvement with unremitting and painful corneal abrasions. Biallelic LAMB3 mutations c.3052-5C>G and c.3492_3493delCG were identified as the molecular basis for this phenotype, with one mutation being a hypomorphic splice variant that allows residual wild-type laminin-332 production. The reduced laminin-332 level was associated with impaired keratinocyte adhesion. Then, we also investigated the therapeutic power of a human amniotic membrane (AM) eyedrop preparation for corneal lesions. AM were isolated from placenta donors, according to a procedure preserving the AM biological characteristics as a tissue, and confirmed to contain laminin-332. We found that AM eyedrop preparation could restore keratinocyte adhesion in an in vitro assay. Of note, AM eyedrop administration to the patient resulted in long-lasting remission of her ocular manifestations. Our findings suggest that AM eyedrops could represent an effective, non-invasive, simple-to-handle treatment for corneal lesions in patients with JEB and possibly other EB forms.

scholarly journals Case report of non-healing surgical wound treated with dehydrated human amniotic membrane

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Neil H Riordan ◽  
Ben A George ◽  
Troy B Chandler ◽  
Randall W McKenna
Keyword(s):  
Case Report ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Surgical Wound

Treatment ofPseudomonas aeruginosakeratitis with combined corneal cross-linking and human amniotic membrane transplantation

2013 ◽  
Vol 91 (5) ◽  
pp. e410-e411 ◽  
Author(s):  
Jaakko S. Mattila ◽  
Anna Korsbäck ◽  
Kari Krootila ◽  
Juha M. Holopainen
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Cross Linking ◽  
Human Amniotic Membrane
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